Gene/Proteome Database (LMPD)
Proteins
| protein kinase C gamma type isoform 1 | |
|---|---|
| Refseq ID | NP_035232 |
| Protein GI | 6755080 |
| UniProt ID | P63318 |
| mRNA ID | NM_011102 |
| Length | 697 |
| RefSeq Status | VALIDATED |
| MAGLGPGGGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTSDEIHITVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTKTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDSKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGSTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIAPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM | |
| protein kinase C gamma type isoform 2 | |
|---|---|
| Refseq ID | NP_001278363 |
| Protein GI | 612149781 |
| UniProt ID | Q2NKI4 |
| mRNA ID | NM_001291434 |
| Length | 646 |
| RefSeq Status | VALIDATED |
| MAGLGPGGGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTSDEIHITVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTKTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYEVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGSTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIAPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005911 | IDA:MGI | C | cell-cell junction |
| GO:0005737 | IDA:UniProtKB | C | cytoplasm |
| GO:0005829 | ISS:UniProtKB | C | cytosol |
| GO:0030425 | IDA:MGI | C | dendrite |
| GO:0005634 | IDA:MGI | C | nucleus |
| GO:0048471 | ISS:UniProtKB | C | perinuclear region of cytoplasm |
| GO:0005886 | IDA:UniProtKB | C | plasma membrane |
| GO:0097060 | IDA:UniProtKB | C | synaptic membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0004698 | IEA:Ensembl | F | calcium-dependent protein kinase C activity |
| GO:0004712 | ISO:MGI | F | protein serine/threonine/tyrosine kinase activity |
| GO:0008270 | IEA:InterPro | F | zinc ion binding |
| GO:0007635 | IMP:MGI | P | chemosensory behavior |
| GO:0060384 | IMP:UniProtKB | P | innervation |
| GO:0035556 | IEA:InterPro | P | intracellular signal transduction |
| GO:0007611 | IEA:Ensembl | P | learning or memory |
| GO:0043524 | ISS:UniProtKB | P | negative regulation of neuron apoptotic process |
| GO:0042177 | IEA:Ensembl | P | negative regulation of protein catabolic process |
| GO:0031397 | IEA:Ensembl | P | negative regulation of protein ubiquitination |
| GO:0032425 | IEA:Ensembl | P | positive regulation of mismatch repair |
| GO:0046777 | IEA:Ensembl | P | protein autophosphorylation |
| GO:0043278 | IMP:UniProtKB | P | response to morphine |
| GO:0048265 | IMP:UniProtKB | P | response to pain |
| GO:0007268 | IMP:UniProtKB | P | synaptic transmission |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR000961 | AGC-kinase, C-terminal |
| IPR000008 | C2 domain |
| IPR020454 | Diacylglycerol/phorbol-ester binding |
| IPR017441 | Protein kinase, ATP binding site |
| IPR014375 | Protein kinase C, alpha/beta/gamma types |
| IPR002219 | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain |
| IPR017892 | Protein kinase, C-terminal |
| IPR000719 | Protein kinase domain |
| IPR011009 | Protein kinase-like domain |
| IPR002290 | Serine/threonine/dual specificity protein kinase, catalytic domain |
| IPR008271 | Serine/threonine-protein kinase, active site |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250}; Note=Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain. {ECO:0000250}; |
| Disruption Phenotype | Mice exhibit impaired motor coordination due to the inability to eliminate multiple climbing fibers during innervation of Purkinje cells. 40% of Purkinje cells are still innervated by multiple climbing fibers. {ECO:0000269|PubMed:8548809}. |
| Enzyme Regulation | Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-514 (activation loop of the kinase domain), Thr-655 (turn motif) and Thr-674 (hydrophobic region), need to be phosphorylated for its full activation. |
| Function | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contribute to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53/TP53 and promotes p53/TP53- dependent apoptosis in response to DNA damage (By similarity). {ECO:0000250}. |
| Ptm | Autophosphorylation on Thr-674 appears to regulate motor functions of junctophilins, JPH3 and JPH4. {ECO:0000269|PubMed:17904530}. |
| Ptm | Ubiquitinated. {ECO:0000250}. |
| Similarity | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. {ECO:0000305}. |
| Similarity | Contains 1 AGC-kinase C-terminal domain. {ECO:0000305}. |
| Similarity | Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}. |
| Similarity | Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}. |
| Similarity | Contains 2 phorbol-ester/DAG-type zinc fingers. {ECO:0000255|PROSITE-ProRule:PRU00226}. |
| Subcellular Location | Cytoplasm. Cytoplasm, perinuclear region {ECO:0000250}. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, dendrite {ECO:0000250}. Note=Translocates to synaptic membranes on stimulation. |
| Subunit | Interacts with CDCP1 and GRIA4. Interacts with TP53INP1 and p53/TP53. {ECO:0000250}. |
| Tissue Specificity | Expressed in brain cerebellum and cortex. Highly expressed in Purkinje cells. {ECO:0000269|PubMed:17904530}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP000809 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 6755080 | RefSeq | NP_035232 | 697 | protein kinase C gamma type isoform 1 |
| 612149781 | RefSeq | NP_001278363 | 646 | protein kinase C gamma type isoform 2 |
Identical Sequences to LMP000809 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6755080 | DBBJ | BAE25881.1 | 697 | unnamed protein product [Mus musculus] |
| GI:6755080 | GenBank | AAH89226.1 | 697 | Protein kinase C, gamma [Rattus norvegicus] |
| GI:612149781 | GenBank | AAI11808.1 | 646 | Prkcc protein [Mus musculus] |
| GI:6755080 | GenBank | ABK42349.1 | 697 | protein kinase C gamma1 [synthetic construct] |
| GI:6755080 | GenBank | EDL31175.1 | 697 | protein kinase C, gamma [Mus musculus] |
| GI:6755080 | GenBank | EDL84948.1 | 697 | protein kinase C, gamma [Rattus norvegicus] |
| GI:612149781 | RefSeq | XP_006228076.1 | 646 | PREDICTED: protein kinase C gamma type isoform X1 [Rattus norvegicus] |
| GI:6755080 | SwissProt | P63318.1 | 697 | RecName: Full=Protein kinase C gamma type; Short=PKC-gamma [Mus musculus] |
Related Sequences to LMP000809 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:612149781 | DBBJ | BAE25881.1 | 697 | unnamed protein product [Mus musculus] |
| GI:6755080 | GenBank | AAA31449.1 | 697 | protein kinase delta [Oryctolagus cuniculus] |
| GI:6755080 | pat | WO | 697 | Sequence 3 from Patent WO 8801303 |
| GI:612149781 | RefSeq | NP_036760.1 | 697 | protein kinase C gamma type [Rattus norvegicus] |
| GI:612149781 | RefSeq | XP_004671631.1 | 646 | PREDICTED: protein kinase C gamma type isoform X2 [Jaculus jaculus] |
| GI:6755080 | RefSeq | XP_004648415.1 | 697 | PREDICTED: protein kinase C gamma type isoform X1 [Octodon degus] |
| GI:612149781 | RefSeq | XP_004648416.1 | 646 | PREDICTED: protein kinase C gamma type isoform X2 [Octodon degus] |
| GI:6755080 | RefSeq | XP_005371458.1 | 697 | PREDICTED: protein kinase C gamma type isoform X1 [Microtus ochrogaster] |
| GI:6755080 | RefSeq | XP_008825950.1 | 697 | PREDICTED: protein kinase C gamma type [Nannospalax galili] |
| GI:6755080 | SwissProt | P10829.1 | 697 | RecName: Full=Protein kinase C gamma type; Short=PKC-gamma; AltName: Full=PKC-delta [Oryctolagus cuniculus] |
| GI:612149781 | SwissProt | P63319.1 | 697 | RecName: Full=Protein kinase C gamma type; Short=PKC-gamma [Rattus norvegicus] |
| GI:612149781 | SwissProt | P63318.1 | 697 | RecName: Full=Protein kinase C gamma type; Short=PKC-gamma [Mus musculus] |