Gene/Proteome Database (LMPD)

LMPD ID
LMP000894
Gene ID
5740
Species
Homo sapiens (Human)
Gene Name
prostaglandin I2 (prostacyclin) synthase
Gene Symbol
PTGIS
Synonyms
CYP8; CYP8A1; PGIS; PTGI
Alternate Names
prostacyclin synthase; prostaglandin I2 synthase; cytochrome P450, family 8, subfamily A, polypeptide 1
Chromosome
20
Map Location
20q13.13
EC Number
5.3.99.4
Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for PTGIS

Proteins

prostacyclin synthase precursor
Refseq ID NP_000952
Protein GI 13699859
UniProt ID Q16647
mRNA ID NM_000961
Length 500
RefSeq Status REVIEWED
MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP
sig_peptide: 1..24 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2649 peptide sequence: MAWAALLGLLAALLLLLLLSRRRT

Gene Information

Entrez Gene ID
5740
Gene Name
prostaglandin I2 (prostacyclin) synthase
Gene Symbol
PTGIS
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005901 IDA:UniProtKB C caveola
GO:0005783 IDA:UniProtKB C endoplasmic reticulum
GO:0005789 TAS:Reactome C endoplasmic reticulum membrane
GO:0005615 ISS:UniProtKB C extracellular space
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005634 IDA:UniProtKB C nucleus
GO:0020037 IDA:UniProtKB F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0004497 IEA:InterPro F monooxygenase activity
GO:0016705 IEA:InterPro F oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0008116 IDA:UniProtKB F prostaglandin-I synthase activity
GO:0097190 IDA:UniProtKB P apoptotic signaling pathway
GO:0019369 TAS:Reactome P arachidonic acid metabolic process
GO:0071456 IDA:UniProtKB P cellular response to hypoxia
GO:0071347 IEP:UniProtKB P cellular response to interleukin-1
GO:0071354 IEP:UniProtKB P cellular response to interleukin-6
GO:0019371 TAS:Reactome P cyclooxygenase pathway
GO:0006690 TAS:Reactome P icosanoid metabolic process
GO:0032088 IDA:UniProtKB P negative regulation of NF-kappaB transcription factor activity
GO:0050728 IDA:UniProtKB P negative regulation of inflammatory response
GO:0045019 IDA:UniProtKB P negative regulation of nitric oxide biosynthetic process
GO:0045766 IMP:UniProtKB P positive regulation of angiogenesis
GO:1900119 IDA:UniProtKB P positive regulation of execution phase of apoptosis
GO:0035360 IDA:UniProtKB P positive regulation of peroxisome proliferator activated receptor signaling pathway
GO:0001516 IDA:UniProtKB P prostaglandin biosynthetic process
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0006805 TAS:Reactome P xenobiotic metabolic process

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_150149 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002403 Cytochrome P450, E-class, group IV
IPR024204 Cytochrome P450, cholesterol 7-alpha-monooxygenase-type
IPR027286 Prostacyclin synthase

UniProt Annotations

Entry Information

Gene Name
prostaglandin I2 (prostacyclin) synthase
Protein Entry
PTGIS_HUMAN
UniProt ID
Q16647
Species
Human

Comments

Comment Type Description
Catalytic Activity (5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15- hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11- alpha,15-dihydroxyprosta-5,13-dienoate.
Cofactor Name=heme; Xref=ChEBI
Function Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).
Similarity Belongs to the cytochrome P450 family.
Subcellular Location Endoplasmic reticulum membrane; Single-pass membrane protein.
Tissue Specificity Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.
Web Resource Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/PTGISID44219ch20q13.html";
Web Resource Name=Cytochrome P450 Allele Nomenclature Committee; Note=CYP8A1 alleles; URL="http://www.cypalleles.ki.se/cyp8a1.htm";

Identical and Related Proteins

Unique RefSeq proteins for LMP000894 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
13699859 RefSeq NP_000952 500 prostacyclin synthase precursor

Identical Sequences to LMP000894 proteins

Reference Database Accession Length Protein Name
GI:13699859 GenBank AAI01812.1 500 Prostaglandin I2 (prostacyclin) synthase [Homo sapiens]
GI:13699859 GenBank ABL13476.1 500 Sequence 15 from patent US 7119176
GI:13699859 GenBank EAW75655.1 500 prostaglandin I2 (prostacyclin) synthase [Homo sapiens]
GI:13699859 GenBank AEN30188.1 500 Sequence 4 from patent US 7994151
GI:13699859 GenBank AFX21883.1 500 Sequence 11 from patent US 8299043
GI:13699859 GenBank AIC49527.1 500 PTGIS, partial [synthetic construct]

Related Sequences to LMP000894 proteins

Reference Database Accession Length Protein Name
GI:13699859 DBBJ BAA28219.1 509 prostacyclin synthase, partial [Homo sapiens]
GI:13699859 DBBJ BAA11910.1 500 prostacyclin synthase [Homo sapiens]
GI:13699859 GenBank AAG31784.1 500 prostacyclin synthase [Homo sapiens]
GI:13699859 GenBank AAG31785.1 500 prostacyclin synthase [Homo sapiens]
GI:13699859 GenBank ADA41312.1 500 Sequence 34 from patent US 7622260
GI:13699859 GenBank AGY20576.1 500 Sequence 34 from patent US 8551700