Gene/Proteome Database (LMPD)

LMPD ID
LMP000895
Gene ID
11688
Species
Mus musculus (Mouse)
Gene Name
arachidonate 8-lipoxygenase
Gene Symbol
Alox8
Synonyms
15-LOX-2; 15-LOX-B; 8-LOX; 8S-LOX; Alox15b
Alternate Names
arachidonate 8S-lipoxygenase; 8S-lipoxygenase; 15-lipoxygenase 2; arachidonate 15-lipoxygenase B; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type
Chromosome
11
Map Location
11|11 B4
EC Number
1.13.11.-
Summary
This gene belongs to the lipoxygenase (LOX) gene family whose members encode enzymes that catalyze the addition of molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield fatty acid hydroperoxides. The encoded enzyme preferentially metabolizes arachidonic acid to yield 8-hydroxyeicosatetraenoic acid (8-HETE), while metabolizing linoleic acid less efficiently. The gene may also function as a tumor suppressor. This gene is located in a cluster of related genes that spans approximately 75 kilobases on chromosome 11. [provided by RefSeq, Jan 2013]
Orthologs
View orthologs and multiple alignments for Alox8

Proteins

arachidonate 8S-lipoxygenase
Refseq ID NP_033791
Protein GI 40363263
UniProt ID O35936
mRNA ID NM_009661
Length 677
RefSeq Status REVIEWED
MAKCRVRVSTGEACGAGTWDKVSVSIVGTHGESPLVPLDHLGKEFSAGAEEDFEVTLPQDVGTVLMLRVHKAPPEVSLPLMSFRSDAWFCRWFELEWLPGAALHFPCYQWLEGAGELVLREGAAKVSWQDHHPTLQDQRQKELESRQKMYSWKTYIEGWPRCLDHETVKDLDLNIKYSAMKNAKLFFKAHSAYTELKVKGLLDRTGLWRSLREMRRLFNFRKTPAAEYVFAHWQEDAFFASQFLNGINPVLIRRCHSLPNNFPVTDEMVAPVLGPGTSLQAELEKGSLFLVDHGILSGVHTNILNGKPQFSAAPMTLLHQSSGSGPLLPIAIQLKQTPGPDNPIFLPSDDTWDWLLAKTWVRNSEFYIHEAVTHLLHAHLIPEVFALATLRQLPRCHPLFKLLIPHIRYTLHINTLARELLVAPGKLIDKSTGLGTGGFSDLIKRNMEQLNYSVLCLPEDIRARGVEDIPGYYYRDDGMQIWGAIKSFVSEIVSIYYPSDTSVQDDQELQAWVREIFSEGFLGRESSGMPSLLDTREALVQYITMVIFTCSAKHAAVSSGQFDSCVWMPNLPPTMQLPPPTSKGQARPESFIATLPAVNSSSYHIIALWLLSAEPGDQRPLGHYPDEHFTEDAPRRSVAAFQRKLIQISKGIRERNRGLALPYTYLDPPLIENSVSI

Gene Information

Entrez Gene ID
11688
Gene Name
arachidonate 8-lipoxygenase
Gene Symbol
Alox8
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005856 IEA:Ensembl C cytoskeleton
GO:0005829 ISS:UniProtKB C cytosol
GO:0019898 IEA:Ensembl C extrinsic component of membrane
GO:0005622 ISS:UniProtKB C intracellular
GO:0005886 IEA:Ensembl C plasma membrane
GO:0050473 IEA:Ensembl F arachidonate 15-lipoxygenase activity
GO:0036403 IDA:UniProtKB F arachidonate 8(S)-lipoxygenase activity
GO:0005509 IEA:Ensembl F calcium ion binding
GO:0005506 IEA:Ensembl F iron ion binding
GO:0016165 IEA:Ensembl F linoleate 13S-lipoxygenase activity
GO:0008289 IEA:UniProtKB-KW F lipid binding
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0043651 IDA:UniProtKB P linoleic acid metabolic process
GO:0019372 IDA:UniProtKB P lipoxygenase pathway
GO:0045786 IEA:Ensembl P negative regulation of cell cycle
GO:0008285 IEA:Ensembl P negative regulation of cell proliferation
GO:0045926 IEA:Ensembl P negative regulation of growth
GO:0090197 IEA:Ensembl P positive regulation of chemokine secretion
GO:0045618 IMP:UniProtKB P positive regulation of keratinocyte differentiation
GO:0010744 IEA:Ensembl P positive regulation of macrophage derived foam cell differentiation
GO:0035360 IMP:UniProtKB P positive regulation of peroxisome proliferator activated receptor signaling pathway

KEGG Pathway Links

KEGG Pathway ID Description
mmu00590 Arachidonic acid metabolism
mmu04726 Serotonergic synapse

REACTOME Pathway Links

REACTOME Pathway ID Description
5894064 Synthesis of 15-eicosatetraenoic acid derivatives

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 8-lipoxygenase
Protein Entry
ALOX8_MOUSE
UniProt ID
O35936
Species
Mouse

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=1.2 uM for arachidonate (at pH 7.4 and 25 degrees Celsius) {ECO:0000269|PubMed:16112079}; KM=2.1 uM for (8S)-HPETE (at pH 7.4 and 25 degrees Celsius) {ECO:0000269|PubMed:16112079}; KM=5.7 uM for (8S)-HETE (at pH 7.4 and 25 degrees Celsius) {ECO:0000269|PubMed:16112079}; KM=39 uM for (15S)-HPETE (at pH 7.4 and 25 degrees Celsius) {ECO:0000269|PubMed:16112079}; KM=15 uM for (15S)-HETE (at pH 7.4 and 25 degrees Celsius) {ECO:0000269|PubMed:16112079}; Note=The highest catalytic efficiency is observed with arachidonate followed by (8S)-HPETE and(15S)-HPETE with similar efficiencies.;
Catalytic Activity Arachidonate + O(2) = (5Z,9E,11Z,14Z)-8- hydroperoxyicosa-5,9,11,14-tetraenoate. {ECO:0000269|PubMed:10625675, ECO:0000269|PubMed:16112079, ECO:0000269|PubMed:16143298}.
Caution Despite its homology with human ALOX15B (AC O15296), it seems not to have any 15S-lipoxygenase activity on arachidonate. Based on its catalytic activity it is referred to as the mouse arachidonate 8S-lipoxygenase. {ECO:0000305}.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|PROSITE-ProRule:PRU00726}; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726};
Domain The PLAT domain can bind calcium ions; this promotes association with membranes. {ECO:0000250}.
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Catalyzes the peroxidation of arachidonate and linoleate into (8S)-HPETE and (9S)-HPODE respectively. From arachidonate mainly produces (8S)-HPETE and in addition, minor products derived from (8S)-HPETE itself that may include leukotriene A4 and 8,15-diHPETE. With arachidonate as substrate, has no detectable 15S-lipoxygenase activity and only displays a 8S-lipoxygenase activity. However, it may have a 15S-lipoxygenase activity with (8S)-HPETE to produce (8S,15S)-diHPETE. May also catalyze (15S)-HPETE peroxidation to produce 8,15-diHPETE. May play a role in keratinocyte differentiation through activation of the peroxisome proliferator activated receptor signaling pathway. {ECO:0000269|PubMed:10625675, ECO:0000269|PubMed:10965849, ECO:0000269|PubMed:16143298, ECO:0000269|PubMed:9305900}.
Induction By phorbol ester. {ECO:0000269|PubMed:9305900}.
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Similarity Belongs to the lipoxygenase family. {ECO:0000305}.
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Subcellular Location Cytoplasm, cytosol {ECO:0000250}. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding. {ECO:0000250}.
Tissue Specificity Expressed in epidermis and brain. No expression found in heart, spleen, liver, skeletal muscle, kidney or testis. {ECO:0000269|PubMed:9305900, ECO:0000269|PubMed:9518531}.

Identical and Related Proteins

Unique RefSeq proteins for LMP000895 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
40363263 RefSeq NP_033791 677 arachidonate 8S-lipoxygenase

Identical Sequences to LMP000895 proteins

Reference Database Accession Length Protein Name
GI:40363263 DBBJ BAC26085.1 677 unnamed protein product [Mus musculus]
GI:40363263 EMBL CAA75003.1 677 arachidonate 8(S)-lipoxygenase [Mus musculus]
GI:40363263 GenBank AAE66005.1 677 Sequence 4 from patent US 6204037
GI:40363263 GenBank AAH15253.1 677 Arachidonate 8-lipoxygenase [Mus musculus]
GI:40363263 GenBank AAR77020.1 677 Sequence 4 from patent US 6649355
GI:40363263 SwissProt O35936.1 677 RecName: Full=Arachidonate 8S-lipoxygenase; Short=8-LOX; Short=8S-LOX; AltName: Full=15-lipoxygenase 2; Short=15-LOX-2; AltName: Full=Arachidonate 15-lipoxygenase B; Short=15-LOX-B [Mus musculus]

Related Sequences to LMP000895 proteins

Reference Database Accession Length Protein Name
GI:40363263 GenBank AAN03708.1 677 15-lipoxygenase-2 [Rattus norvegicus]
GI:40363263 GenBank EDL10483.1 684 arachidonate 8-lipoxygenase, partial [Mus musculus]
GI:40363263 GenBank EDM04847.1 677 arachidonate 15-lipoxygenase, second type [Rattus norvegicus]
GI:40363263 RefSeq NP_695213.1 677 arachidonate 15-lipoxygenase B [Rattus norvegicus]
GI:40363263 RefSeq XP_005349852.1 677 PREDICTED: arachidonate 15-lipoxygenase B isoform X3 [Microtus ochrogaster]
GI:40363263 SwissProt Q8K4F2.1 677 RecName: Full=Arachidonate 15-lipoxygenase B; Short=15-LOX-B; AltName: Full=15-lipoxygenase 2; Short=15-LOX-2; AltName: Full=Arachidonate 15-lipoxygenase type II; AltName: Full=Linoleate 13-lipoxygenase 15-LOb [Rattus norvegicus]