Gene/Proteome Database (LMPD)
LMPD ID
LMP000923
Gene ID
Species
Homo sapiens (Human)
Gene Name
diacylglycerol kinase, theta 110kDa
Gene Symbol
Synonyms
DAGK; DAGK4; DAGK7
Alternate Names
diacylglycerol kinase theta; DGK-theta; DAG kinase theta; diglyceride kinase theta
Chromosome
4
Map Location
4p16.3
EC Number
2.7.1.107
Summary
The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| diacylglycerol kinase theta | |
|---|---|
| Refseq ID | NP_001338 |
| Protein GI | 40806175 |
| UniProt ID | P52824 |
| mRNA ID | NM_001347 |
| Length | 942 |
| RefSeq Status | REVIEWED |
| MAAAAEPGARAWLGGGSPRPGSPACSPVLGSGGRARPGPGPGPGPERAGVRAPGPAAAPGHSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRIPCTSVAPSLVRVPVAHCFGPRGLHKRKFCAVCRKVLEAPALHCEVCELHLHPDCVPFACSDCRQCHQDGHQDHDTHHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSLCSAALAPECGFGRLRSLVLPPACVRLLPGGFSKTQSFRIVEAAEPGEGGDGADGSAAVGPGRETQATPESGKQTLKIFDGDDAVRRSQFRLVTVSRLAGAEEVLEAALRAHHIPEDPGHLELCRLPPSSQACDAWAGGKAGSAVISEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVRVTPKSTARSVVLEVLPLLGRQAESPESFQLVEVAMGCRHVQRTMLMDEQPLLDRLQDIRQMSVRQVSQTRFYVAESRDVAPHVSLFVGGLPPGLSPEEYSSLLHEAGATKATVVSVSHIYSSQGAVVLDVACFAEAERLYMLLKDMAVRGRLLTALVLPDLLHAKLPPDSCPLLVFVNPKSGGLKGRDLLCSFRKLLNPHQVFDLTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIPSWGSGADLWGSDSDTRFEKPRMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKAKQKPRRAGTTRDARADAAPAPESDPR | |
Gene Information
Entrez Gene ID
Gene Name
diacylglycerol kinase, theta 110kDa
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:UniProtKB | C | cytoplasm |
| GO:0005856 | IDA:UniProtKB | C | cytoskeleton |
| GO:0005829 | IDA:UniProtKB | C | cytosol |
| GO:0016607 | IDA:UniProtKB | C | nuclear speck |
| GO:0005634 | IDA:UniProtKB | C | nucleus |
| GO:0005886 | IDA:UniProtKB | C | plasma membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0003951 | IEA:InterPro | F | NAD+ kinase activity |
| GO:0033613 | IPI:UniProtKB | F | activating transcription factor binding |
| GO:0004143 | IDA:UniProtKB | F | diacylglycerol kinase activity |
| GO:0019900 | IPI:UniProtKB | F | kinase binding |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0043274 | IPI:UniProtKB | F | phospholipase binding |
| GO:0007186 | IMP:UniProtKB | P | G-protein coupled receptor signaling pathway |
| GO:0007596 | TAS:Reactome | P | blood coagulation |
| GO:0019933 | IDA:UniProtKB | P | cAMP-mediated signaling |
| GO:0030168 | TAS:Reactome | P | platelet activation |
| GO:0070528 | IDA:UniProtKB | P | protein kinase C signaling |
| GO:0007205 | IEA:InterPro | P | protein kinase C-activating G-protein coupled receptor signaling pathway |
| GO:0006357 | IDA:UniProtKB | P | regulation of transcription from RNA polymerase II promoter |
| GO:0033198 | IDA:UniProtKB | P | response to ATP |
| GO:0070493 | IDA:UniProtKB | P | thrombin receptor signaling pathway |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa00561 | Glycerolipid metabolism |
| hsa00564 | Glycerophospholipid metabolism |
| hsa01100 | Metabolic pathways |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_19184 | GPCR downstream signaling |
Domain Information
InterPro Annotations
UniProt Annotations
Entry Information
Gene Name
diacylglycerol kinase, theta 110kDa
Protein Entry
DGKQ_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2- diacyl-sn-glycerol 3-phosphate. |
| Domain | The L-X-X-L-L repeats are implicated in binding to the nuclear receptor NR5A1. |
| Enzyme Regulation | Inactivated by binding to RHOA. Not inhibited by phosphatidylserine. |
| Function | Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. |
| Ptm | Phosphorylated by PRKCE and PRKCH in vitro. |
| Similarity | Belongs to the eukaryotic diacylglycerol kinase family. |
| Similarity | Contains 1 DAGKc domain. {ECO |
| Similarity | Contains 1 Ras-associating domain. |
| Similarity | Contains 3 phorbol-ester/DAG-type zinc fingers. |
| Subcellular Location | Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton. Nucleus. Nucleus speckle. Note=Translocates to the nucleus in response to thrombin stimulation (By similarity). Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH. |
| Subunit | Interacts with RHOA (constitutively activated, GTP- bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1; the interaction requires the LXXLL motif 1 and LXXLL motif 2 in DGKQ. {ECO |
Identical and Related Proteins
Unique RefSeq proteins for LMP000923 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 40806175 | RefSeq | NP_001338 | 942 | diacylglycerol kinase theta |
Identical Sequences to LMP000923 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:40806175 | GenBank | ADS18174.1 | 942 | Sequence 31 from patent US 7763418 |
| GI:40806175 | GenBank | AHD72628.1 | 942 | Sequence 9696 from patent US 8586006 |
| GI:40806175 | SwissProt | P52824.2 | 942 | RecName: Full=Diacylglycerol kinase theta; Short=DAG kinase theta; AltName: Full=Diglyceride kinase theta; Short=DGK-theta [Homo sapiens] |
Related Sequences to LMP000923 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:40806175 | DBBJ | BAG10554.1 | 942 | diacylglycerol kinase theta, partial [synthetic construct] |
| GI:40806175 | GenBank | AAA98749.1 | 942 | diacylglycerol kinase [Homo sapiens] |
| GI:40806175 | GenBank | AAH63801.1 | 942 | Diacylglycerol kinase, theta 110kDa [Homo sapiens] |
| GI:40806175 | GenBank | EAW82632.1 | 941 | diacylglycerol kinase, theta 110kDa, isoform CRA_c [Homo sapiens] |
| GI:40806175 | GenBank | ADU87648.1 | 942 | epididymis tissue sperm binding protein Li 19mP [Homo sapiens] |
| GI:40806175 | PRF | - | 942 | diacylglycerol kinase 4 [Homo sapiens] |