Gene/Proteome Database (LMPD)

LMPD ID
LMP000962
Gene ID
240
Species
Homo sapiens (Human)
Gene Name
arachidonate 5-lipoxygenase
Gene Symbol
ALOX5
Synonyms
5-LO; 5-LOX; 5LPG; LOG5
Alternate Names
arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform
Chromosome
10
Map Location
10q11.2
EC Number
1.13.11.34
Summary
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Orthologs
View orthologs and multiple alignments for ALOX5

Proteins

arachidonate 5-lipoxygenase isoform 1
Refseq ID NP_000689
Protein GI 4502057
UniProt ID P09917
mRNA ID NM_000698
Length 674
RefSeq Status REVIEWED
MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI
arachidonate 5-lipoxygenase isoform 2
Refseq ID NP_001243082
Protein GI 371877525
UniProt ID P09917
mRNA ID NM_001256153
Length 642
RefSeq Status REVIEWED
MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI
arachidonate 5-lipoxygenase isoform 3
Refseq ID NP_001243083
Protein GI 371877598
UniProt ID P09917
mRNA ID NM_001256154
Length 617
RefSeq Status REVIEWED
MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQLFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI

Gene Information

Entrez Gene ID
240
Gene Name
arachidonate 5-lipoxygenase
Gene Symbol
ALOX5
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:UniProtKB C cytosol
GO:0005615 IDA:UniProt C extracellular space
GO:0005635 IDA:UniProtKB C nuclear envelope
GO:0005641 IDA:UniProtKB C nuclear envelope lumen
GO:0016363 IDA:UniProtKB C nuclear matrix
GO:0031965 IDA:UniProtKB C nuclear membrane
GO:0004051 IDA:UniProtKB F arachidonate 5-lipoxygenase activity
GO:0005506 IDA:UniProtKB F iron ion binding
GO:0019369 TAS:Reactome P arachidonic acid metabolic process
GO:0019370 IDA:UniProtKB P leukotriene biosynthetic process
GO:0006691 TAS:Reactome P leukotriene metabolic process
GO:0002540 IEA:Ensembl P leukotriene production involved in inflammatory response
GO:2001300 TAS:Reactome P lipoxin metabolic process
GO:0019372 TAS:Reactome P lipoxygenase pathway
GO:0044281 TAS:Reactome P small molecule metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
hsa04913 Ovarian steroidogenesis
hsa04726 Serotonergic synapse
hsa05145 Toxoplasmosis

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 5-lipoxygenase
Protein Entry
LOX5_HUMAN
UniProt ID
P09917
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=5; Comment=Additional isoforms seem to exist.; Name=1; IsoId=P09917-1; Sequence=Displayed; Name=2; Synonyms=Delta-13; IsoId=P09917-2; Sequence=VSP_046998; Name=3; Synonyms=delta-p10; IsoId=P09917-3; Sequence=VSP_053534; Name=4; Synonyms=delta-10-13; IsoId=P09917-4; Sequence=VSP_053535, VSP_053537; Name=5; Synonyms=alpha-10; IsoId=P09917-5; Sequence=VSP_053536;
Catalytic Activity Arachidonate + O(2) = leukotriene A(4) + H(2)O.
Cofactor Name=Ca(2+); Xref=ChEBI
Cofactor Name=Fe cation; Xref=ChEBI
Function Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Interaction Q14019:COTL1; NbExp=4; IntAct=EBI-79934, EBI-79926; P09769:FGR; NbExp=2; IntAct=EBI-79934, EBI-1383732; P08631:HCK; NbExp=2; IntAct=EBI-79934, EBI-346340; P17612:PRKACA; NbExp=2; IntAct=EBI-79934, EBI-476586; P07947:YES1; NbExp=2; IntAct=EBI-79934, EBI-515331;
Pathway Lipid metabolism; leukotriene A4 biosynthesis.
Ptm Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. {ECO
Similarity Belongs to the lipoxygenase family.
Similarity Contains 1 PLAT domain. {ECO
Similarity Contains 1 lipoxygenase domain. {ECO
Subcellular Location Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Note=Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Subunit Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity. {ECO
Web Resource Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/ALOX5ID42985ch10q11.html";

Identical and Related Proteins

Unique RefSeq proteins for LMP000962 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
4502057 RefSeq NP_000689 674 arachidonate 5-lipoxygenase isoform 1
371877525 RefSeq NP_001243082 642 arachidonate 5-lipoxygenase isoform 2
371877598 RefSeq NP_001243083 617 arachidonate 5-lipoxygenase isoform 3

Identical Sequences to LMP000962 proteins

Reference Database Accession Length Protein Name
GI:371877598 DBBJ BAJ20691.1 617 arachidonate 5-lipoxygenase, partial [synthetic construct]
GI:371877598 GenBank AAI43986.1 617 ALOX5 protein [Homo sapiens]
GI:4502057 GenBank ACM80482.1 674 Sequence 5980 from patent US 6812339
GI:371877598 GenBank ADR30799.1 617 arachidonic 5-lipoxygenase delta-13 isoform [Homo sapiens]
GI:371877525 GenBank ADR30801.1 642 arachidonic 5-lipoxygenase delta-p10 isoform [Homo sapiens]
GI:4502057 GenBank ADR83089.1 674 arachidonate 5-lipoxygenase, partial [synthetic construct]
GI:4502057 GenBank ADS96629.1 674 Sequence 2 from patent US 7829535
GI:4502057 GenBank AFD45206.1 674 Sequence 199 from patent US 8129114
GI:4502057 GenBank AHD69733.1 674 Sequence 1322 from patent US 8586006
GI:371877598 GenBank AIB02645.1 617 Sequence 2 from patent US 8669289
GI:4502057 GenBank AIC48253.1 674 ALOX5, partial [synthetic construct]

Related Sequences to LMP000962 proteins

Reference Database Accession Length Protein Name
GI:371877525 EMBL CAD12173.1 674 unnamed protein product [Homo sapiens]
GI:371877525 GenBank AAA36183.1 674 lipoxygenase [Homo sapiens]
GI:371877525 GenBank AAA65450.1 674 5-lipoxygenase [Homo sapiens]
GI:371877525 GenBank AAI30333.1 674 Arachidonate 5-lipoxygenase [Homo sapiens]
GI:371877525 GenBank ADR83089.1 674 arachidonate 5-lipoxygenase, partial [synthetic construct]
GI:4502057 RefSeq XP_001102354.1 674 PREDICTED: arachidonate 5-lipoxygenase isoform 1 [Macaca mulatta]
GI:371877598 RefSeq XP_002805673.1 617 PREDICTED: arachidonate 5-lipoxygenase isoform 2 [Macaca mulatta]
GI:4502057 RefSeq XP_003816801.1 674 PREDICTED: arachidonate 5-lipoxygenase [Pan paniscus]
GI:4502057 RefSeq XP_003903645.1 674 PREDICTED: arachidonate 5-lipoxygenase [Papio anubis]
GI:4502057 RefSeq XP_004049388.1 760 PREDICTED: arachidonate 5-lipoxygenase [Gorilla gorilla gorilla]
GI:371877598 RefSeq XP_004432487.1 617 PREDICTED: arachidonate 5-lipoxygenase isoform 3 [Ceratotherium simum simum]
GI:371877598 RefSeq XP_004765938.1 617 PREDICTED: arachidonate 5-lipoxygenase isoform X3 [Mustela putorius furo]
GI:371877598 RefSeq XP_004815316.1 617 PREDICTED: arachidonate 5-lipoxygenase isoform X3 [Mustela putorius furo]
GI:4502057 RefSeq XP_005565166.1 674 PREDICTED: arachidonate 5-lipoxygenase isoform X1 [Macaca fascicularis]
GI:371877598 RefSeq XP_005565168.1 617 PREDICTED: arachidonate 5-lipoxygenase isoform X3 [Macaca fascicularis]
GI:4502057 RefSeq XP_007960729.1 674 PREDICTED: arachidonate 5-lipoxygenase [Chlorocebus sabaeus]
GI:371877598 RefSeq XP_008835957.1 617 PREDICTED: arachidonate 5-lipoxygenase isoform X3 [Nannospalax galili]
GI:371877525 SwissProt P09917.2 674 RecName: Full=Arachidonate 5-lipoxygenase; Short=5-LO; Short=5-lipoxygenase [Homo sapiens]