Gene/Proteome Database (LMPD)
LMPD ID
LMP001043
Gene ID
Species
Homo sapiens (Human)
Gene Name
choline kinase alpha
Gene Symbol
Synonyms
CHK; CK; CKI; EK
Alternate Names
choline kinase alpha; CHETK-alpha; ethanolamine kinase
Chromosome
11
Map Location
11q13.2
EC Number
2.7.1.32
Summary
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| choline kinase alpha isoform a | |
|---|---|
| Refseq ID | NP_001268 |
| Protein GI | 47078276 |
| UniProt ID | P35790 |
| mRNA ID | NM_001277 |
| Length | 457 |
| RefSeq Status | REVIEWED |
| MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPPPPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKEGSEQAQKENEFQGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV | |
| choline kinase alpha isoform b | |
|---|---|
| Refseq ID | NP_997634 |
| Protein GI | 47078278 |
| UniProt ID | P35790 |
| mRNA ID | NM_212469 |
| Length | 439 |
| RefSeq Status | REVIEWED |
| MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPPPPLPLPLPLPQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | TAS:Reactome | C | cytosol |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0033265 | IEA:Ensembl | F | choline binding |
| GO:0004103 | IDA:UniProtKB | F | choline kinase activity |
| GO:0004104 | IEA:Ensembl | F | cholinesterase activity |
| GO:0008144 | IDA:UniProtKB | F | drug binding |
| GO:0004305 | IDA:UniProtKB | F | ethanolamine kinase activity |
| GO:0004871 | TAS:ProtInc | F | signal transducer activity |
| GO:0006657 | IDA:GOC | P | CDP-choline pathway |
| GO:0019695 | IEA:Ensembl | P | choline metabolic process |
| GO:0046474 | TAS:Reactome | P | glycerophospholipid biosynthetic process |
| GO:0006629 | TAS:ProtInc | P | lipid metabolic process |
| GO:0006869 | TAS:ProtInc | P | lipid transport |
| GO:0006656 | IDA:UniProtKB | P | phosphatidylcholine biosynthetic process |
| GO:0006646 | IDA:UniProtKB | P | phosphatidylethanolamine biosynthetic process |
| GO:0006644 | TAS:Reactome | P | phospholipid metabolic process |
| GO:0007165 | TAS:GOC | P | signal transduction |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_121238 | Synthesis of PC |
| REACT_120919 | Synthesis of PE |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR011009 | Protein kinase-like domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P35790-1; Sequence=Displayed; Name=2; IsoId=P35790-2; Sequence=VSP_009683; |
| Biophysicochemical Properties | Kinetic parameters: KM=0.2 mM for choline {ECO |
| Catalytic Activity | ATP + choline = ADP + phosphocholine. |
| Catalytic Activity | ATP + ethanolamine = ADP + O- phosphoethanolamine. |
| Function | Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. |
| Pathway | Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from choline: step 1/1. |
| Pathway | Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3. |
| Similarity | Belongs to the choline/ethanolamine kinase family. |
| Subcellular Location | Cytoplasm. |
| Subunit | Heterodimer with CHKB (By similarity). Homodimer. {ECO |
| Web Resource | Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/CHKAID44009ch11q13.html"; |
Identical and Related Proteins
Unique RefSeq proteins for LMP001043 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 47078276 | RefSeq | NP_001268 | 457 | choline kinase alpha isoform a |
| 47078278 | RefSeq | NP_997634 | 439 | choline kinase alpha isoform b |
Identical Sequences to LMP001043 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:47078276 | GenBank | AHD76977.1 | 457 | Sequence 22110 from patent US 8586006 |
| GI:47078278 | GenBank | AHD76978.1 | 439 | Sequence 22111 from patent US 8586006 |
| GI:47078276 | PDB | 4DA5 | 457 | Chain A, Choline Kinase Alpha Acts Through A Double-displacement Kinetic Mechanism Involving Enzyme Isomerisation, As Determined Through Enzyme And Inhibitor Kinetics And Structural Biology |
| GI:47078276 | PDB | 4DA5 | 457 | Chain B, Choline Kinase Alpha Acts Through A Double-displacement Kinetic Mechanism Involving Enzyme Isomerisation, As Determined Through Enzyme And Inhibitor Kinetics And Structural Biology |
| GI:47078276 | SwissProt | P35790.3 | 457 | RecName: Full=Choline kinase alpha; Short=CK; AltName: Full=CHETK-alpha; AltName: Full=Ethanolamine kinase; Short=EK [Homo sapiens] |
Related Sequences to LMP001043 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:47078276 | DBBJ | BAA01547.1 | 456 | choline kinase [Homo sapiens] |
| GI:47078276 | DBBJ | BAK62755.1 | 455 | choline kinase alpha [Pan troglodytes] |
| GI:47078278 | GenBank | AAH36471.1 | 439 | Choline kinase alpha [Homo sapiens] |
| GI:47078276 | GenBank | JAA22515.1 | 455 | choline kinase alpha [Pan troglodytes] |
| GI:47078278 | GenBank | AHD76977.1 | 457 | Sequence 22110 from patent US 8586006 |
| GI:47078278 | PDB | 4DA5 | 457 | Chain A, Choline Kinase Alpha Acts Through A Double-displacement Kinetic Mechanism Involving Enzyme Isomerisation, As Determined Through Enzyme And Inhibitor Kinetics And Structural Biology |
| GI:47078278 | PDB | 4DA5 | 457 | Chain B, Choline Kinase Alpha Acts Through A Double-displacement Kinetic Mechanism Involving Enzyme Isomerisation, As Determined Through Enzyme And Inhibitor Kinetics And Structural Biology |
| GI:47078276 | PRF | - | 456 | choline kinase [Homo sapiens] |
| GI:47078278 | RefSeq | NP_001268.2 | 457 | choline kinase alpha isoform a [Homo sapiens] |
| GI:47078276 | RefSeq | NP_001267005.1 | 455 | choline kinase alpha [Pan troglodytes] |
| GI:47078276 | RefSeq | XP_007960466.1 | 584 | PREDICTED: choline kinase alpha isoform X1 [Chlorocebus sabaeus] |
| GI:47078278 | SwissProt | P35790.3 | 457 | RecName: Full=Choline kinase alpha; Short=CK; AltName: Full=CHETK-alpha; AltName: Full=Ethanolamine kinase; Short=EK [Homo sapiens] |