Gene/Proteome Database (LMPD)

LMPD ID
LMP001229
Gene ID
8473
Species
Homo sapiens (Human)
Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase
Gene Symbol
OGT
Synonyms
HRNT1; O-GLCNAC
Alternate Names
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Chromosome
X
Map Location
Xq13
EC Number
2.4.1.255
Summary
This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Orthologs
View orthologs and multiple alignments for OGT

Proteins

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 1
Refseq ID NP_858058
Protein GI 32307148
UniProt ID O15294
mRNA ID NM_181672
Length 1046
RefSeq Status REVIEWED
MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2
Refseq ID NP_858059
Protein GI 32307150
UniProt ID O15294
mRNA ID NM_181673
Length 1036
RefSeq Status REVIEWED
MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA

Gene Information

Entrez Gene ID
8473
Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase
Gene Symbol
OGT
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:HPA C cytoplasm
GO:0005829 IDA:UniProtKB C cytosol
GO:0000123 IDA:UniProtKB C histone acetyltransferase complex
GO:0005815 IDA:HPA C microtubule organizing center
GO:0005739 IEA:UniProtKB-KW C mitochondrion
GO:0070688 IDA:UniProtKB C MLL5-L complex
GO:0005654 TAS:Reactome C nucleoplasm
GO:0005634 IDA:UniProtKB C nucleus
GO:0005886 IDA:UniProtKB C plasma membrane
GO:0008375 TAS:ProtInc F acetylglucosaminyltransferase activity
GO:0008047 IDA:BHF-UCL F enzyme activator activity
GO:0005547 IDA:UniProtKB F phosphatidylinositol-3,4,5-trisphosphate binding
GO:0016262 IDA:UniProtKB F protein N-acetylglucosaminyltransferase activity
GO:0097363 ISS:UniProtKB F protein O-GlcNAc transferase activity
GO:0006915 IDA:UniProtKB P apoptotic process
GO:0071300 IMP:BHF-UCL P cellular response to retinoic acid
GO:0006325 TAS:Reactome P chromatin organization
GO:0032922 ISS:UniProtKB P circadian regulation of gene expression
GO:0080182 IMP:UniProtKB P histone H3-K4 trimethylation
GO:0043984 IDA:UniProtKB P histone H4-K16 acetylation
GO:0043981 IDA:UniProtKB P histone H4-K5 acetylation
GO:0043982 IDA:UniProtKB P histone H4-K8 acetylation
GO:0031397 ISS:UniProtKB P negative regulation of protein ubiquitination
GO:0048015 IDA:UniProtKB P phosphatidylinositol-mediated signaling
GO:0043085 IDA:GOC P positive regulation of catalytic activity
GO:0030854 IMP:BHF-UCL P positive regulation of granulocyte differentiation
GO:0061087 IMP:UniProtKB P positive regulation of histone H3-K27 methylation
GO:0051571 IDA:BHF-UCL P positive regulation of histone H3-K4 methylation
GO:0045862 IDA:UniProtKB P positive regulation of proteolysis
GO:0045944 IDA:UniProtKB P positive regulation of transcription from RNA polymerase II promoter
GO:0006493 IDA:UniProtKB P protein O-linked glycosylation
GO:0090526 ISS:UniProtKB P regulation of gluconeogenesis involved in cellular glucose homeostasis
GO:0006110 IDA:UniProtKB P regulation of glycolytic process
GO:0046626 IDA:UniProtKB P regulation of insulin receptor signaling pathway
GO:0035020 IDA:UniProtKB P regulation of Rac protein signal transduction
GO:0032868 IDA:UniProtKB P response to insulin
GO:0007584 TAS:ProtInc P response to nutrient
GO:0007165 TAS:ProtInc P signal transduction

KEGG Pathway Links

KEGG Pathway ID Description
hsa00514 Other types of O-glycan biosynthesis
ko00514 Other types of O-glycan biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_172633 Chromatin modifying enzymes
REACT_172610 HATs acetylate histones

Domain Information

InterPro Annotations

Accession Description
IPR029489 O-GlcNAc transferase, C-terminal
IPR011990 Tetratricopeptide-like helical domain
IPR019734 Tetratricopeptide repeat
IPR013026 Tetratricopeptide repeat-containing domain
IPR001440 Tetratricopeptide TPR1

UniProt Annotations

Entry Information

Gene Name
O-linked N-acetylglucosamine (GlcNAc) transferase
Protein Entry
OGT1_HUMAN
UniProt ID
O15294
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=4; Name=3; Synonyms=Nucleocytoplasmic isoform, ncOGT; IsoId=O15294-1; Sequence=Displayed; Name=2; Synonyms=Mitochondrial isoform, mOGT; IsoId=O15294-2; Sequence=VSP_006553; Name=1; IsoId=O15294-3; Sequence=VSP_014164; Name=4; Synonyms=Short isoform, sOGT; IsoId=O15294-4; Sequence=VSP_040764; Note=No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=1.8 uM for UDP-N-acetyl-D-glucosamine ;
Catalytic Activity UDP-N-acetyl-D-glucosamine + [protein]-L- serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine.
Catalytic Activity UDP-N-acetyl-D-glucosamine + [protein]-L- threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L- threonine.
Disease Note=Regulation of OGT activity and altered O- GlcNAcylations are implicated in diabetes and Alzheimer disease. O-GlcNAcylation of AKT1 affects insulin signaling and, possibly diabetes. Reduced O-GlcNAcylations and resulting increased phosphorylations of MAPT/TAU are observed in Alzheimer disease (AD) brain cerebrum.
Domain The TPR repeat domain is required for substrate binding and oligomerization.
Enzyme Regulation Subject to product inhibition by UDP.
Function Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity (PubMed:22923583). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B
Function Isoform 2: the mitochondrial isoform (mOGT) is cytotoxic and triggers apoptosis in several cell types including INS1, an insulinoma cell line.
Induction Induction of the nucleocytoplasmic OGT (ncOGT) isoform in the liver on glucose deprivation is mediated by the decreased hexosamine biosynthesis pathway (HBP) flux.
Interaction P51610:HCFC1; NbExp=9; IntAct=EBI-539828, EBI-396176; P09022:Hoxa1 (xeno); NbExp=3; IntAct=EBI-539828, EBI-3957603; O95644:NFATC1; NbExp=2; IntAct=EBI-539828, EBI-6907210; Q04206:RELA; NbExp=2; IntAct=EBI-539828, EBI-73886; Q6N021:TET2; NbExp=7; IntAct=EBI-539828, EBI-310727; O43151:TET3; NbExp=4; IntAct=EBI-539828, EBI-2831148; Q8BG87:Tet3 (xeno); NbExp=2; IntAct=EBI-539828, EBI-9031997;
Pathway Protein modification; protein glycosylation.
Ptm Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.
Ptm Ubiquitinated, leading to its proteasomal degradation.
Similarity Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.
Similarity Contains 13 TPR repeats. {ECO
Subcellular Location Isoform 2: Mitochondrion. Membrane. Note=Associates with the mitochondrial inner membrane.
Subcellular Location Isoform 3: Cytoplasm. Nucleus. Cell membrane. Note=Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1. After insulin induction, translocated from the nucleus to the cell membrane via phophatidylinisotide binding. Colocalizes with AKT1 at the plasma membrane.
Subcellular Location Isoform 4: Cytoplasm. Nucleus.
Subunit Heterotrimer; consists of one 78 kDa subunit and two 110 kDa subunits dimerized via TPR repeats 6 and 7. Interacts (via TPR repeats 6 and 7) with ATXN10 (By similarity). Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, HCFC1, PPP1CC and ACTB. Component of a THAP1/THAP3-HCFC1- OGT complex. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts directly with HCFC1; the interaction O- glycosylates HCFC1, regulates its proteolytic processing and transcriptional activity and, in turn, stabilizes OGT in the nucleus. Interacts (via TPRs 1-6) with SIN3A; the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts (via TPR 5-6) with TET1, TET2 and TET3. Interacts with ARNTL/BMAL1 (By similarity).
Tissue Specificity Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
Web Resource Name=Functional Glycomics Gateway - GTase; Note=UDP- N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110kDa subunit; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_554";

Identical and Related Proteins

Unique RefSeq proteins for LMP001229 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
32307148 RefSeq NP_858058 1046 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 1
32307150 RefSeq NP_858059 1036 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2

Identical Sequences to LMP001229 proteins

Reference Database Accession Length Protein Name
GI:32307148 GenBank JAA11099.1 1046 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) [Pan troglodytes]
GI:32307150 GenBank JAA11100.1 1036 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) [Pan troglodytes]
GI:32307148 GenBank JAA11101.1 1046 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) [Pan troglodytes]
GI:32307150 GenBank JAA16524.1 1036 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) [Pan troglodytes]
GI:32307148 GenBank JAA16525.1 1046 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) [Pan troglodytes]
GI:32307148 GenBank JAA40056.1 1046 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) [Pan troglodytes]
GI:32307150 GenBank AGM65000.1 1036 Sequence 49 from patent US 8383425
GI:32307150 RefSeq XP_003820180.1 1036 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Pan paniscus]
GI:32307148 RefSeq XP_004064413.1 1046 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 1 [Gorilla gorilla gorilla]
GI:32307150 RefSeq XP_004064414.1 1036 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2 [Gorilla gorilla gorilla]
GI:32307148 RefSeq XP_008069102.1 1046 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Tarsius syrichta]
GI:32307150 RefSeq XP_008069103.1 1036 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Tarsius syrichta]

Related Sequences to LMP001229 proteins

Reference Database Accession Length Protein Name
GI:32307150 GenBank JAB20770.1 1036 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2 [Callithrix jacchus]
GI:32307150 GenBank JAB33157.1 1036 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2 [Callithrix jacchus]
GI:32307148 RefSeq XP_002720149.1 1046 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Oryctolagus cuniculus]
GI:32307148 RefSeq XP_002763023.1 1046 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Callithrix jacchus]
GI:32307148 RefSeq XP_003471044.1 1046 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2 [Cavia porcellus]
GI:32307148 RefSeq XP_004022227.1 1046 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2 [Ovis aries]
GI:32307148 RefSeq XP_004646842.1 1046 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Octodon degus]
GI:32307150 RefSeq XP_005339999.1 1036 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Ictidomys tridecemlineatus]
GI:32307150 RefSeq XP_005401730.1 1036 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Chinchilla lanigera]
GI:32307150 RefSeq XP_005593977.1 1036 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Macaca fascicularis]
GI:32307150 RefSeq XP_005673770.1 1036 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X2 [Sus scrofa]
GI:32307148 RefSeq XP_008155829.1 1046 PREDICTED: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform X1 [Eptesicus fuscus]