Gene/Proteome Database (LMPD)
LMPD ID
LMP001233
Gene ID
Species
Homo sapiens (Human)
Gene Name
arachidonate 15-lipoxygenase, type B
Gene Symbol
Synonyms
15-LOX-2
Alternate Names
arachidonate 15-lipoxygenase B; 15-LOX-B; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; linoleate 13-lipoxygenase 15-LOb; arachidonate omega(6) lipoxygenase; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type
Chromosome
17
Map Location
17p13.1
EC Number
1.13.11.33
Summary
This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| arachidonate 15-lipoxygenase B isoform a | |
|---|---|
| Refseq ID | NP_001034219 |
| Protein GI | 85067499 |
| UniProt ID | O15296 |
| mRNA ID | NM_001039130 |
| Length | 647 |
| RefSeq Status | REVIEWED |
| MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQAELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGAVERFVSEIIGIYYPSDESVQDDRELQAWVREIFSKGFLNQESSGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQERNQGLVLPYTYLDPPLIENSVSI | |
| arachidonate 15-lipoxygenase B isoform b | |
|---|---|
| Refseq ID | NP_001034220 |
| Protein GI | 85067503 |
| UniProt ID | O15296 |
| mRNA ID | NM_001039131 |
| Length | 602 |
| RefSeq Status | REVIEWED |
| MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQAELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQERNQGLVLPYTYLDPPLIENSVSI | |
| arachidonate 15-lipoxygenase B isoform d | |
|---|---|
| Refseq ID | NP_001132 |
| Protein GI | 85067501 |
| UniProt ID | O15296 |
| mRNA ID | NM_001141 |
| Length | 676 |
| RefSeq Status | REVIEWED |
| MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQAELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKLLIPHTRYTLHINTLARELLIVPGQVVDRSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGAVERFVSEIIGIYYPSDESVQDDRELQAWVREIFSKGFLNQESSGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQERNQGLVLPYTYLDPPLIENSVSI | |
Gene Information
Entrez Gene ID
Gene Name
arachidonate 15-lipoxygenase, type B
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:HPA | C | cytoplasm |
| GO:0005856 | IDA:UniProtKB | C | cytoskeleton |
| GO:0005829 | IDA:UniProtKB | C | cytosol |
| GO:0070062 | IDA:UniProtKB | C | extracellular vesicular exosome |
| GO:0019898 | IDA:UniProtKB | C | extrinsic component of membrane |
| GO:0005622 | IDA:UniProtKB | C | intracellular |
| GO:0016020 | IDA:UniProtKB | C | membrane |
| GO:0005634 | IDA:UniProtKB | C | nucleus |
| GO:0005886 | IDA:UniProtKB | C | plasma membrane |
| GO:0050473 | IDA:UniProtKB | F | arachidonate 15-lipoxygenase activity |
| GO:0036403 | IEA:Ensembl | F | arachidonate 8(S)-lipoxygenase activity |
| GO:0005509 | IDA:UniProtKB | F | calcium ion binding |
| GO:0005506 | IDA:UniProtKB | F | iron ion binding |
| GO:0016165 | IDA:UniProtKB | F | linoleate 13S-lipoxygenase activity |
| GO:0008289 | IEA:UniProtKB-KW | F | lipid binding |
| GO:0006915 | TAS:UniProtKB | P | apoptotic process |
| GO:0019369 | IDA:UniProtKB | P | arachidonic acid metabolic process |
| GO:0051122 | ISS:UniProtKB | P | hepoxilin biosynthetic process |
| GO:0043651 | IEA:Ensembl | P | linoleic acid metabolic process |
| GO:0006629 | TAS:UniProtKB | P | lipid metabolic process |
| GO:0019372 | TAS:Reactome | P | lipoxygenase pathway |
| GO:0045786 | IDA:UniProtKB | P | negative regulation of cell cycle |
| GO:0030336 | TAS:UniProtKB | P | negative regulation of cell migration |
| GO:0008285 | IDA:UniProtKB | P | negative regulation of cell proliferation |
| GO:0045926 | IDA:UniProtKB | P | negative regulation of growth |
| GO:0090197 | IMP:UniProtKB | P | positive regulation of chemokine secretion |
| GO:0045618 | IEA:Ensembl | P | positive regulation of keratinocyte differentiation |
| GO:0010744 | IMP:UniProtKB | P | positive regulation of macrophage derived foam cell differentiation |
| GO:0035360 | IEA:Ensembl | P | positive regulation of peroxisome proliferator activated receptor signaling pathway |
| GO:0030850 | NAS:UniProtKB | P | prostate gland development |
| GO:0030856 | NAS:UniProtKB | P | regulation of epithelial cell differentiation |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa04726 | Serotonergic synapse |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_150422 | Synthesis of 15-eicosatetraenoic acid derivatives |
Domain Information
UniProt Annotations
Entry Information
Gene Name
arachidonate 15-lipoxygenase, type B
Protein Entry
LX15B_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=4; Name=A; Synonyms=15-LOb1; IsoId=O15296-1; Sequence=Displayed; Name=B; Synonyms=15-LOX2sv-b; IsoId=O15296-2; Sequence=VSP_003142, VSP_003143; Name=C; Synonyms=15-LOX2sv-c; IsoId=O15296-3; Sequence=VSP_003144, VSP_003145; Name=D; Synonyms=15-LOX2sv-a, 15-LOb2; IsoId=O15296-4; Sequence=VSP_003142; |
| Biophysicochemical Properties | Kinetic parameters: KM=1100 uM for arachidonate (isoform D at pH 7.4 and 20 degrees Celsius) ; KM=10 uM for linoleate (isoform A at pH 7.4 and 20 degrees Celsius) ; KM=25 uM for arachidonate (isoform A at pH 7.4 and 20 degrees Celsius) ; Vmax=4 umol/min/mg enzyme with arachidonate as substrate (isoform A at pH 7.4 and 20 degrees Celsius) ; Vmax=2 umol/min/mg enzyme with arachidonate as substrate (isoform D at pH 7.4 and 20 degrees Celsius) ; Vmax=4 umol/min/mg enzyme with linoleate as substrate (isoform A at pH 7.4 and 20 degrees Celsius) ; |
| Catalytic Activity | Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)- 15-hydroperoxyicosa-5,8,11,13-tetraenoate. |
| Catalytic Activity | Linoleate + O(2) = (9Z,11E)-13- hydroxyoctadeca-9,11-dienoate. |
| Caution | Despite its homology with mouse Alox8 (AC O35936), it seems not to have any 8S-lipoxygenase activity on arachidonate. |
| Cofactor | Name=Fe cation; Xref=ChEBI |
| Domain | The PLAT domain can bind calcium ions; this promotes association with membranes. |
| Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)- HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells. {ECO |
| Induction | Up-regulated by UV-irradiation. |
| Pathway | Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis. |
| Sequence Caution | Sequence=AAD37786.1; Type=Erroneous gene model prediction; Evidence= ; |
| Similarity | Belongs to the lipoxygenase family. |
| Similarity | Contains 1 lipoxygenase domain. {ECO |
| Similarity | Contains 1 PLAT domain. {ECO |
| Subcellular Location | Cytoplasm, cytosol. Cell membrane. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding. |
| Subcellular Location | Isoform A |
| Tissue Specificity | Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea. {ECO |
Identical and Related Proteins
Unique RefSeq proteins for LMP001233 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 85067499 | RefSeq | NP_001034219 | 647 | arachidonate 15-lipoxygenase B isoform a |
| 85067503 | RefSeq | NP_001034220 | 602 | arachidonate 15-lipoxygenase B isoform b |
| 85067501 | RefSeq | NP_001132 | 676 | arachidonate 15-lipoxygenase B isoform d |
Identical Sequences to LMP001233 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:85067501 | SwissProt | O15296.3 | 676 | RecName: Full=Arachidonate 15-lipoxygenase B; Short=15-LOX-B; AltName: Full=15-lipoxygenase 2; Short=15-LOX-2; AltName: Full=Arachidonate 15-lipoxygenase type II; AltName: Full=Linoleate 13-lipoxygenase 15-LOb [Homo sapiens] |
Related Sequences to LMP001233 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:85067499 | GenBank | AAL76275.1 | 647 | 15-lipoxygenase 2 splice variant a [Homo sapiens] |
| GI:85067503 | GenBank | AAL76275.1 | 647 | 15-lipoxygenase 2 splice variant a [Homo sapiens] |
| GI:85067503 | GenBank | AAL76276.1 | 602 | 15-lipoxygenase 2 splice variant b [Homo sapiens] |
| GI:85067501 | GenBank | AAH35217.1 | 676 | Arachidonate 15-lipoxygenase, type B [Homo sapiens] |
| GI:85067501 | GenBank | EAW90098.1 | 676 | arachidonate 15-lipoxygenase, type B, isoform CRA_b [Homo sapiens] |
| GI:85067501 | GenBank | EAW90100.1 | 676 | arachidonate 15-lipoxygenase, type B, isoform CRA_b [Homo sapiens] |
| GI:85067503 | GenBank | EAW90101.1 | 602 | arachidonate 15-lipoxygenase, type B, isoform CRA_d [Homo sapiens] |
| GI:85067499 | GenBank | EAW90102.1 | 647 | arachidonate 15-lipoxygenase, type B, isoform CRA_e [Homo sapiens] |
| GI:85067503 | GenBank | EAW90102.1 | 647 | arachidonate 15-lipoxygenase, type B, isoform CRA_e [Homo sapiens] |
| GI:85067501 | GenBank | ABW03380.1 | 676 | arachidonate 15-lipoxygenase, type B, partial [synthetic construct] |
| GI:85067501 | GenBank | AIC53989.1 | 676 | ALOX15B, partial [synthetic construct] |
| GI:85067499 | PDB | 4NRE | 696 | Chain A, The Structure Of Human 15-lipoxygenase-2 With A Substrate Mimic |
| GI:85067501 | PDB | 4NRE | 696 | Chain A, The Structure Of Human 15-lipoxygenase-2 With A Substrate Mimic |
| GI:85067503 | RefSeq | NP_001034219.1 | 647 | arachidonate 15-lipoxygenase B isoform a [Homo sapiens] |
| GI:85067499 | RefSeq | NP_001132.2 | 676 | arachidonate 15-lipoxygenase B isoform d [Homo sapiens] |
| GI:85067499 | RefSeq | XP_003818096.1 | 647 | PREDICTED: arachidonate 15-lipoxygenase B isoform X3 [Pan paniscus] |
| GI:85067503 | RefSeq | XP_004058582.1 | 602 | PREDICTED: arachidonate 15-lipoxygenase B isoform 3 [Gorilla gorilla gorilla] |
| GI:85067499 | SwissProt | O15296.3 | 676 | RecName: Full=Arachidonate 15-lipoxygenase B; Short=15-LOX-B; AltName: Full=15-lipoxygenase 2; Short=15-LOX-2; AltName: Full=Arachidonate 15-lipoxygenase type II; AltName: Full=Linoleate 13-lipoxygenase 15-LOb [Homo sapiens] |