Gene/Proteome Database (LMPD)

LMPD ID
LMP001233
Gene ID
247
Species
Homo sapiens (Human)
Gene Name
arachidonate 15-lipoxygenase, type B
Gene Symbol
Synonyms
15-LOX-2
Alternate Names
arachidonate 15-lipoxygenase B; 15-LOX-B; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; linoleate 13-lipoxygenase 15-LOb; arachidonate omega(6) lipoxygenase; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type
Chromosome
17
Map Location
17p13.1
EC Number
1.13.11.33
Summary
This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Orthologs

Proteins

arachidonate 15-lipoxygenase B isoform a
Refseq ID NP_001034219
Protein GI 85067499
UniProt ID O15296
mRNA ID NM_001039130
Length 647
RefSeq Status REVIEWED
MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQAELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGAVERFVSEIIGIYYPSDESVQDDRELQAWVREIFSKGFLNQESSGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQERNQGLVLPYTYLDPPLIENSVSI
arachidonate 15-lipoxygenase B isoform b
Refseq ID NP_001034220
Protein GI 85067503
UniProt ID O15296
mRNA ID NM_001039131
Length 602
RefSeq Status REVIEWED
MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQAELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQERNQGLVLPYTYLDPPLIENSVSI
arachidonate 15-lipoxygenase B isoform d
Refseq ID NP_001132
Protein GI 85067501
UniProt ID O15296
mRNA ID NM_001141
Length 676
RefSeq Status REVIEWED
MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQAELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKLLIPHTRYTLHINTLARELLIVPGQVVDRSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGAVERFVSEIIGIYYPSDESVQDDRELQAWVREIFSKGFLNQESSGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQERNQGLVLPYTYLDPPLIENSVSI

Gene Information

Entrez Gene ID
247
Gene Name
arachidonate 15-lipoxygenase, type B
Gene Symbol
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:HPA C cytoplasm
GO:0005856 IDA:UniProtKB C cytoskeleton
GO:0005829 IDA:UniProtKB C cytosol
GO:0070062 IDA:UniProtKB C extracellular vesicular exosome
GO:0019898 IDA:UniProtKB C extrinsic component of membrane
GO:0005622 IDA:UniProtKB C intracellular
GO:0016020 IDA:UniProtKB C membrane
GO:0005634 IDA:UniProtKB C nucleus
GO:0005886 IDA:UniProtKB C plasma membrane
GO:0050473 IDA:UniProtKB F arachidonate 15-lipoxygenase activity
GO:0036403 IEA:Ensembl F arachidonate 8(S)-lipoxygenase activity
GO:0005509 IDA:UniProtKB F calcium ion binding
GO:0005506 IDA:UniProtKB F iron ion binding
GO:0016165 IDA:UniProtKB F linoleate 13S-lipoxygenase activity
GO:0008289 IEA:UniProtKB-KW F lipid binding
GO:0006915 TAS:UniProtKB P apoptotic process
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0051122 ISS:UniProtKB P hepoxilin biosynthetic process
GO:0043651 IEA:Ensembl P linoleic acid metabolic process
GO:0006629 TAS:UniProtKB P lipid metabolic process
GO:0019372 TAS:Reactome P lipoxygenase pathway
GO:0045786 IDA:UniProtKB P negative regulation of cell cycle
GO:0030336 TAS:UniProtKB P negative regulation of cell migration
GO:0008285 IDA:UniProtKB P negative regulation of cell proliferation
GO:0045926 IDA:UniProtKB P negative regulation of growth
GO:0090197 IMP:UniProtKB P positive regulation of chemokine secretion
GO:0045618 IEA:Ensembl P positive regulation of keratinocyte differentiation
GO:0010744 IMP:UniProtKB P positive regulation of macrophage derived foam cell differentiation
GO:0035360 IEA:Ensembl P positive regulation of peroxisome proliferator activated receptor signaling pathway
GO:0030850 NAS:UniProtKB P prostate gland development
GO:0030856 NAS:UniProtKB P regulation of epithelial cell differentiation
GO:0044281 TAS:Reactome P small molecule metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
hsa04726 Serotonergic synapse

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_150422 Synthesis of 15-eicosatetraenoic acid derivatives

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 15-lipoxygenase, type B
Protein Entry
LX15B_HUMAN
UniProt ID
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=4; Name=A; Synonyms=15-LOb1; IsoId=O15296-1; Sequence=Displayed; Name=B; Synonyms=15-LOX2sv-b; IsoId=O15296-2; Sequence=VSP_003142, VSP_003143; Name=C; Synonyms=15-LOX2sv-c; IsoId=O15296-3; Sequence=VSP_003144, VSP_003145; Name=D; Synonyms=15-LOX2sv-a, 15-LOb2; IsoId=O15296-4; Sequence=VSP_003142;
Biophysicochemical Properties Kinetic parameters: KM=1100 uM for arachidonate (isoform D at pH 7.4 and 20 degrees Celsius) ; KM=10 uM for linoleate (isoform A at pH 7.4 and 20 degrees Celsius) ; KM=25 uM for arachidonate (isoform A at pH 7.4 and 20 degrees Celsius) ; Vmax=4 umol/min/mg enzyme with arachidonate as substrate (isoform A at pH 7.4 and 20 degrees Celsius) ; Vmax=2 umol/min/mg enzyme with arachidonate as substrate (isoform D at pH 7.4 and 20 degrees Celsius) ; Vmax=4 umol/min/mg enzyme with linoleate as substrate (isoform A at pH 7.4 and 20 degrees Celsius) ;
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)- 15-hydroperoxyicosa-5,8,11,13-tetraenoate.
Catalytic Activity Linoleate + O(2) = (9Z,11E)-13- hydroxyoctadeca-9,11-dienoate.
Caution Despite its homology with mouse Alox8 (AC O35936), it seems not to have any 8S-lipoxygenase activity on arachidonate.
Cofactor Name=Fe cation; Xref=ChEBI
Domain The PLAT domain can bind calcium ions; this promotes association with membranes.
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)- HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells. {ECO
Induction Up-regulated by UV-irradiation.
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Sequence Caution Sequence=AAD37786.1; Type=Erroneous gene model prediction; Evidence= ;
Similarity Belongs to the lipoxygenase family.
Similarity Contains 1 PLAT domain. {ECO
Similarity Contains 1 lipoxygenase domain. {ECO
Subcellular Location Cytoplasm, cytosol. Cell membrane. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding.
Subcellular Location Isoform A
Tissue Specificity Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea. {ECO

Identical and Related Proteins

Unique RefSeq proteins for LMP001233 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
85067499 RefSeq NP_001034219 647 arachidonate 15-lipoxygenase B isoform a
85067503 RefSeq NP_001034220 602 arachidonate 15-lipoxygenase B isoform b
85067501 RefSeq NP_001132 676 arachidonate 15-lipoxygenase B isoform d

Identical Sequences to LMP001233 proteins

Reference Database Accession Length Protein Name
GI:85067501 SwissProt O15296.3 676 RecName: Full=Arachidonate 15-lipoxygenase B; Short=15-LOX-B; AltName: Full=15-lipoxygenase 2; Short=15-LOX-2; AltName: Full=Arachidonate 15-lipoxygenase type II; AltName: Full=Linoleate 13-lipoxygenase 15-LOb [Homo sapiens]

Related Sequences to LMP001233 proteins

Reference Database Accession Length Protein Name
GI:85067503 GenBank AAL76275.1 647 15-lipoxygenase 2 splice variant a [Homo sapiens]
GI:85067499 GenBank AAL76275.1 647 15-lipoxygenase 2 splice variant a [Homo sapiens]
GI:85067503 GenBank AAL76276.1 602 15-lipoxygenase 2 splice variant b [Homo sapiens]
GI:85067501 GenBank AAH35217.1 676 Arachidonate 15-lipoxygenase, type B [Homo sapiens]
GI:85067501 GenBank EAW90098.1 676 arachidonate 15-lipoxygenase, type B, isoform CRA_b [Homo sapiens]
GI:85067501 GenBank EAW90100.1 676 arachidonate 15-lipoxygenase, type B, isoform CRA_b [Homo sapiens]
GI:85067503 GenBank EAW90101.1 602 arachidonate 15-lipoxygenase, type B, isoform CRA_d [Homo sapiens]
GI:85067503 GenBank EAW90102.1 647 arachidonate 15-lipoxygenase, type B, isoform CRA_e [Homo sapiens]
GI:85067499 GenBank EAW90102.1 647 arachidonate 15-lipoxygenase, type B, isoform CRA_e [Homo sapiens]
GI:85067501 GenBank ABW03380.1 676 arachidonate 15-lipoxygenase, type B, partial [synthetic construct]
GI:85067501 GenBank AIC53989.1 676 ALOX15B, partial [synthetic construct]
GI:85067501 PDB 4NRE 696 Chain A, The Structure Of Human 15-lipoxygenase-2 With A Substrate Mimic
GI:85067499 PDB 4NRE 696 Chain A, The Structure Of Human 15-lipoxygenase-2 With A Substrate Mimic
GI:85067503 RefSeq NP_001034219.1 647 arachidonate 15-lipoxygenase B isoform a [Homo sapiens]
GI:85067499 RefSeq NP_001132.2 676 arachidonate 15-lipoxygenase B isoform d [Homo sapiens]
GI:85067499 RefSeq XP_003818096.1 647 PREDICTED: arachidonate 15-lipoxygenase B isoform X3 [Pan paniscus]
GI:85067503 RefSeq XP_004058582.1 602 PREDICTED: arachidonate 15-lipoxygenase B isoform 3 [Gorilla gorilla gorilla]
GI:85067499 SwissProt O15296.3 676 RecName: Full=Arachidonate 15-lipoxygenase B; Short=15-LOX-B; AltName: Full=15-lipoxygenase 2; Short=15-LOX-2; AltName: Full=Arachidonate 15-lipoxygenase type II; AltName: Full=Linoleate 13-lipoxygenase 15-LOb [Homo sapiens]