Gene/Proteome Database (LMPD)
LMPD ID
LMP001239
Gene ID
Species
Homo sapiens (Human)
Gene Name
protein arginine methyltransferase 1
Gene Symbol
Synonyms
ANM1; HCP1; HRMT1L2; IR1B4
Chromosome
19
Map Location
19q13.3
EC Number
2.1.1.-
Summary
This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
Orthologs
Proteins
| protein arginine N-methyltransferase 1 isoform 1 | |
|---|---|
| Refseq ID | NP_001527 |
| Protein GI | 154759421 |
| UniProt ID | Q99873 |
| mRNA ID | NM_001536 |
| Length | 371 |
| RefSeq Status | REVIEWED |
| MAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR | |
| protein arginine N-methyltransferase 1 isoform 3 | |
|---|---|
| Refseq ID | NP_938074 |
| Protein GI | 151301219 |
| UniProt ID | Q99873 |
| mRNA ID | NM_198318 |
| Length | 353 |
| RefSeq Status | REVIEWED |
| MAAAEAANCIMEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR | |
Gene Information
Entrez Gene ID
Gene Name
protein arginine methyltransferase 1
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | TAS:ProtInc | C | cytoplasm |
| GO:0005829 | IBA:RefGenome | C | cytosol |
| GO:0005634 | IDA:MGI | C | nucleus |
| GO:0008170 | IDA:UniProtKB | F | N-methyltransferase activity |
| GO:0042054 | IDA:UniProtKB | F | histone methyltransferase activity |
| GO:0044020 | IDA:UniProtKB | F | histone methyltransferase activity (H4-R3 specific) |
| GO:0042802 | IPI:IntAct | F | identical protein binding |
| GO:0008168 | TAS:ProtInc | F | methyltransferase activity |
| GO:0044822 | IDA:UniProtKB | F | poly(A) RNA binding |
| GO:0035242 | IBA:RefGenome | F | protein-arginine omega-N asymmetric methyltransferase activity |
| GO:0007166 | TAS:ProtInc | P | cell surface receptor signaling pathway |
| GO:0043985 | IDA:UniProtKB | P | histone H4-R3 methylation |
| GO:0016571 | IDA:UniProtKB | P | histone methylation |
| GO:0045653 | IDA:UniProtKB | P | negative regulation of megakaryocyte differentiation |
| GO:0031175 | IMP:UniProtKB | P | neuron projection development |
| GO:0018216 | IDA:UniProtKB | P | peptidyl-arginine methylation |
| GO:0019919 | IBA:RefGenome | P | peptidyl-arginine methylation, to asymmetrical-dimethyl arginine |
| GO:0006479 | TAS:ProtInc | P | protein methylation |
| GO:0006355 | IBA:RefGenome | P | regulation of transcription, DNA-templated |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa04068 | FoxO signaling pathway |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_228108 | RMTs methylate histone arginines |
Domain Information
UniProt Annotations
Entry Information
Gene Name
protein arginine methyltransferase 1
Protein Entry
ANM1_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=4; Name=1; Synonyms=V2; IsoId=Q99873-1; Sequence=Displayed; Name=2; Synonyms=V3; IsoId=Q99873-2; Sequence=VSP_005208; Name=3; Synonyms=V1; IsoId=Q99873-3; Sequence=VSP_005209; Name=4; IsoId=Q99873-4; Sequence=VSP_046334; |
| Biophysicochemical Properties | Kinetic parameters: KM=1 uM for AdoMet ; KM=4.2 uM for H4 ; Vmax=1.2 nmol/min/mg enzyme toward AdoMet ; Vmax=1.24 nmol/min/mg enzyme toward H4 ; |
| Catalytic Activity | S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone]. {ECO |
| Enzyme Regulation | By BTG1, BTG2 and ILF3. |
| Function | Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. {ECO |
| Interaction | Self; NbExp=3; IntAct=EBI-78738, EBI-78738; Q9Y3Y2:CHTOP; NbExp=2; IntAct=EBI-78738, EBI-347794; Q5TAQ9:DCAF8; NbExp=2; IntAct=EBI-78738, EBI-740686; Q08211:DHX9; NbExp=2; IntAct=EBI-78738, EBI-352022; Q01844:EWSR1; NbExp=2; IntAct=EBI-78738, EBI-739737; Q8IZU1:FAM9A; NbExp=2; IntAct=EBI-78738, EBI-8468186; P04591:gag (xeno); NbExp=2; IntAct=EBI-78738, EBI-6179727; Q5JVS0:HABP4; NbExp=2; IntAct=EBI-78738, EBI-523625; P61978:HNRNPK; NbExp=3; IntAct=EBI-78738, EBI-304185; Q12906:ILF3; NbExp=2; IntAct=EBI-78738, EBI-78756; P48552:NRIP1; NbExp=4; IntAct=EBI-78738, EBI-746484; Q8IZS5:OFCC1; NbExp=2; IntAct=EBI-78738, EBI-8477661; Q9NR22:PRMT8; NbExp=3; IntAct=EBI-78738, EBI-745545; Q14524:SCN5A; NbExp=2; IntAct=EBI-78738, EBI-726858; Q96BD6:SPSB1; NbExp=2; IntAct=EBI-78738, EBI-2659201; Q99619:SPSB2; NbExp=2; IntAct=EBI-78738, EBI-2323209; O60506:SYNCRIP; NbExp=2; IntAct=EBI-78738, EBI-1024357; P40337:VHL; NbExp=2; IntAct=EBI-78738, EBI-301246; |
| Similarity | Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family. |
| Similarity | Contains 1 SAM-dependent MTase PRMT-type domain. |
| Subcellular Location | Nucleus . Nucleus, nucleoplasm . Cytoplasm, cytosol . Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity). |
| Subunit | Homodimer and heterodimer with PRMT8. Individual homodimers can associate to form a homohexamer. Interacts with BTG1, BTG2, NFATC2IP and IFNAR1 (By similarity). Interacts with and methylates CHTOP, thereby enabling the interaction of CHTOP with the 5FMC complex (By similarity). Interacts with ILF3 and SUPT5H. Interacts with and methylates FOXO1, leading to the nuclear retention of FOXO1 and the stimulation of FOXO1 transcriptional activity. Methylation of FOXO1 is increased upon oxidative stress. {ECO |
| Tissue Specificity | Widely expressed. |
Identical and Related Proteins
Unique RefSeq proteins for LMP001239 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 154759421 | RefSeq | NP_001527 | 371 | protein arginine N-methyltransferase 1 isoform 1 |
| 151301219 | RefSeq | NP_938074 | 353 | protein arginine N-methyltransferase 1 isoform 3 |
Identical Sequences to LMP001239 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:151301219 | RefSeq | XP_006744380.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Leptonychotes weddellii] |
| GI:154759421 | RefSeq | XP_006868268.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Chrysochloris asiatica] |
| GI:151301219 | RefSeq | XP_006868269.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Chrysochloris asiatica] |
| GI:154759421 | RefSeq | XP_006905082.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Pteropus alecto] |
| GI:151301219 | RefSeq | XP_006905083.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Pteropus alecto] |
| GI:154759421 | RefSeq | XP_006080146.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Bubalus bubalis] |
| GI:151301219 | RefSeq | XP_006080147.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Bubalus bubalis] |
| GI:154759421 | RefSeq | XP_008046591.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Tarsius syrichta] |
| GI:151301219 | RefSeq | XP_008046592.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Tarsius syrichta] |
| GI:154759421 | RefSeq | XP_009434335.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 [Pan troglodytes] |
| GI:154759421 | RefSeq | XP_010367802.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Rhinopithecus roxellana] |
| GI:151301219 | RefSeq | XP_010367803.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Rhinopithecus roxellana] |
Related Sequences to LMP001239 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:151301219 | RefSeq | XP_003406850.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1-like isoform 1 [Loxodonta africana] |
| GI:154759421 | RefSeq | XP_003406851.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1-like isoform 2 [Loxodonta africana] |
| GI:154759421 | RefSeq | XP_004381695.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform 1 [Trichechus manatus latirostris] |
| GI:151301219 | RefSeq | XP_004381696.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform 2 [Trichechus manatus latirostris] |
| GI:154759421 | RefSeq | XP_004619900.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Sorex araneus] |
| GI:151301219 | RefSeq | XP_004619901.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Sorex araneus] |
| GI:154759421 | RefSeq | XP_004710499.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Echinops telfairi] |
| GI:151301219 | RefSeq | XP_004710500.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Echinops telfairi] |
| GI:154759421 | RefSeq | XP_004694345.1 | 371 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Condylura cristata] |
| GI:151301219 | RefSeq | XP_007941042.1 | 353 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Orycteropus afer afer] |
| GI:154759421 | RefSeq | XP_008573608.1 | 373 | PREDICTED: protein arginine N-methyltransferase 1 isoform X1 [Galeopterus variegatus] |
| GI:151301219 | RefSeq | XP_008573609.1 | 355 | PREDICTED: protein arginine N-methyltransferase 1 isoform X2 [Galeopterus variegatus] |