Gene/Proteome Database (LMPD)

LMPD ID
LMP001311
Gene ID
74769
Species
Mus musculus (Mouse)
Gene Name
phosphatidylinositol 3-kinase, catalytic, beta polypeptide
Gene Symbol
Pik3cb
Synonyms
1110001J02Rik; AI447572; p110beta
Alternate Names
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3Kbeta; PI3K-beta; PI3-kinase subunit beta; ptdIns-3-kinase subunit beta; ptdIns-3-kinase subunit p110-beta; phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Chromosome
9
Map Location
9|9 E4
EC Number
2.7.1.153

Proteins

phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Refseq ID NP_083370
Protein GI 269914109
UniProt ID Q8BTI9
mRNA ID NM_029094
Length 1064
RefSeq Status VALIDATED
MPPAMADNLDIWAVDSQIASDGAISVDFLLPTGIYIQLEVPREATISYIKQMLWKQVHNYPMFNLLMDIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPAEKLDSKIGVLIGKGLHEFDALKDPEVNEFRRKMRKFSEAKIQSLVGLSWIDWLKHTYPPEHEPSVLENLEDKLYGGKLVVAVHFENSQDVFSFQVSPNLNPIKINELAIQKRLTIRGKEDEASPCDYVLQVSGRVEYVFGDHPLIQFQYIRNCVMNRTLPHFILVECCKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTRVISHIWDNNNPFQITLVKGNKLNTEETVKVHVRAGLFHGTELLCKTVVSSEISGKNDHIWNEQLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTINPSKYQTIRKAGKVHYPVAWVNTMVFDFKGQLRSGDVILHSWSSFPDELEEMLNPMGTVQTNPYAENATALHITFPENKKQPCYYPPFDKIIEKAAELASGDSANVSSRGGKKFLAVLKEILDRDPLSQLCENEMDLIWTLRQDCRENFPQSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLRQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALDNRRIGQFLFWHLRSEVHTPAVSVQFGVILEAYCRGSVGHMKVLSKQVEALNKLKTLNSLIKLNAVKLSRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYSSRAFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAATAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKSEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS

Gene Information

Entrez Gene ID
74769
Gene Name
phosphatidylinositol 3-kinase, catalytic, beta polypeptide
Gene Symbol
Pik3cb
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0005942 IBA:RefGenome C phosphatidylinositol 3-kinase complex
GO:0005886 IBA:RefGenome C plasma membrane
GO:0016303 IMP:MGI F 1-phosphatidylinositol-3-kinase activity
GO:0035005 IBA:RefGenome F 1-phosphatidylinositol-4-phosphate 3-kinase activity
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0043560 IPI:MGI F insulin receptor substrate binding
GO:0046934 IBA:RefGenome F phosphatidylinositol-4,5-bisphosphate 3-kinase activity
GO:0006914 IEA:UniProtKB-KW P autophagy
GO:0006874 IMP:MGI P cellular calcium ion homeostasis
GO:0040016 IMP:MGI P embryonic cleavage
GO:0006897 IEA:UniProtKB-KW P endocytosis
GO:0007156 IMP:MGI P homophilic cell adhesion via plasma membrane adhesion molecules
GO:0036092 IMP:GOC P phosphatidylinositol-3-phosphate biosynthetic process
GO:0048015 IEA:InterPro P phosphatidylinositol-mediated signaling
GO:0030168 IMP:MGI P platelet activation
GO:0001952 IMP:MGI P regulation of cell-matrix adhesion

KEGG Pathway Links

KEGG Pathway ID Description
mmu04152 AMPK signaling pathway
mmu04024 cAMP signaling pathway
mmu04022 cGMP-PKG signaling pathway
mmu04550 Signaling pathways regulating pluripotency of stem cells

Domain Information

InterPro Annotations

Accession Description
IPR016024 Armadillo-type fold
IPR000008 C2 domain
IPR000403 Phosphatidylinositol 3-/4-kinase, catalytic domain
IPR018936 Phosphatidylinositol 3/4-kinase, conserved site
IPR003113 Phosphatidylinositol 3-kinase adaptor-binding (PI3K ABD) domain
IPR002420 Phosphatidylinositol 3-kinase, C2 domain
IPR000341 Phosphatidylinositol 3-kinase Ras-binding (PI3K RBD) domain
IPR015433 Phosphatidylinositol Kinase
IPR001263 Phosphoinositide 3-kinase, accessory (PIK) domain
IPR011009 Protein kinase-like domain
IPR029071 Ubiquitin-related domain

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol 3-kinase, catalytic, beta polypeptide
Protein Entry
PK3CB_MOUSE
UniProt ID
Q8BTI9
Species
Mouse

Comments

Comment Type Description
Catalytic Activity ATP + 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5- trisphosphate.
Disruption Phenotype Mice have defects in autophagosome formation. Have normal bleeding time but are resistant to thrombosis after arterial injury. Mice fail to induce tumors in a model of prostate tumor formation induced by Pten loss. {ECO:0000269|PubMed:18594509, ECO:0000269|PubMed:19515725, ECO:0000269|PubMed:20065293, ECO:0000269|PubMed:21059846}.
Domain The inhibitory interactions with PIK3R1 are mediated by the PI3K-ABD domain and the C2 PI3K-type domain with the iSH2 (inter-SH2) region of PIK3R1; the C2 PI3K-type domain, the PI3K helical domain, and the PI3K/PI4K kinase domain with the nSH2 (N- terminal SH2) region of PIK3R1; and the PI3K/PI4K kinase domain with the cSH2 (C-terminal SH2) region of PIK3R1. The inhibitory interaction between the PI3K-ABD domain and the C2 PI3K-type domain with the iSH2 (inter-SH2) region of PIK3R1 is weak. The nuclear localization signal (NLS) is required for its function in cell survival (By similarity). {ECO:0000250}.
Function Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors. {ECO:0000269|PubMed:18544649, ECO:0000269|PubMed:18594509, ECO:0000269|PubMed:19515725, ECO:0000269|PubMed:19940148, ECO:0000269|PubMed:20065293, ECO:0000269|PubMed:21059846}.
Interaction P26450:Pik3r1; NbExp=4; IntAct=EBI-644672, EBI-641764;
Pathway Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Ptm Phosphorylation at Ser-1064 down-regulates lipid kinase activity. {ECO:0000250}.
Similarity Belongs to the PI3/PI4-kinase family. {ECO:0000255|PROSITE-ProRule:PRU00877, ECO:0000255|PROSITE- ProRule:PRU00879, ECO:0000255|PROSITE-ProRule:PRU00880}.
Similarity Contains 1 C2 PI3K-type domain. {ECO:0000255|PROSITE- ProRule:PRU00880}.
Similarity Contains 1 PI3K-ABD domain. {ECO:0000255|PROSITE- ProRule:PRU00877}.
Similarity Contains 1 PI3K/PI4K domain. {ECO:0000255|PROSITE- ProRule:PRU00269}.
Similarity Contains 1 PI3K-RBD domain. {ECO:0000255|PROSITE- ProRule:PRU00879}.
Similarity Contains 1 PIK helical domain. {ECO:0000255|PROSITE- ProRule:PRU00878}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Note=Interaction with PIK3R2 is required for nuclear localization and export.
Subunit Heterodimer of a catalytic subunit PIK3CB and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interaction with PIK3R2 is required for nuclear localization and nuclear export (By similarity). Part of a complex with PIK3R1 and PTEN (By similarity). Binding to PTEN may antagonize the lipid kinase activity under normal growth conditions (By similarity). Part of a complex involved in autophagosome formation composed of PIK3C3 and PIK3R4. Interacts with BECN1, ATG14 and RAB5A. {ECO:0000250, ECO:0000269|PubMed:21059846}.

Identical and Related Proteins

Unique RefSeq proteins for LMP001311 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
269914109 RefSeq NP_083370 1064 phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform

Identical Sequences to LMP001311 proteins

Reference Database Accession Length Protein Name
GI:269914109 DBBJ BAE32380.1 1064 unnamed protein product [Mus musculus]
GI:269914109 GenBank ABK42310.1 1064 phosphoinositide 3-kinase p110 beta, class Ia catalytic subunit [synthetic construct]
GI:269914109 GenBank EDL20998.1 1064 phosphatidylinositol 3-kinase, catalytic, beta polypeptide [Mus musculus]
GI:269914109 SwissProt Q8BTI9.2 1064 RecName: Full=Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; Short=PI3-kinase subunit beta; Short=PI3K-beta; Short=PI3Kbeta; Short=PtdIns-3-kinase subunit beta; AltName: Full=Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; Short=PtdIns-3-kinase subunit p110-beta; Short=p110beta [Mus musculus]

Related Sequences to LMP001311 proteins

Reference Database Accession Length Protein Name
GI:269914109 DBBJ BAC41102.1 1064 unnamed protein product [Mus musculus]
GI:269914109 EMBL CAA10046.1 1070 phosphatidylinositol 3-kinase, catalytic subunit, beta isoform [Rattus norvegicus]
GI:269914109 GenBank EDL77452.1 1070 phosphatidylinositol 3-kinase, catalytic, beta polypeptide [Rattus norvegicus]
GI:269914109 RefSeq NP_445933.1 1070 phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform [Rattus norvegicus]
GI:269914109 RefSeq XP_006243704.1 1070 PREDICTED: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform X1 [Rattus norvegicus]
GI:269914109 SwissProt Q9Z1L0.1 1070 RecName: Full=Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; Short=PI3-kinase subunit beta; Short=PI3K-beta; Short=PI3Kbeta; Short=PtdIns-3-kinase subunit beta; AltName: Full=Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; Short=PtdIns-3-kinase subunit p110-beta; Short=p110beta [Rattus norvegicus]