Gene/Proteome Database (LMPD)

LMPD ID
LMP001328
Gene ID
18706
Species
Mus musculus (Mouse)
Gene Name
phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
Gene Symbol
Pik3ca
Synonyms
6330412C24Rik; caPI3K; p110; p110alpha
Alternate Names
phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3Kalpha; PI3K-alpha; PI3-kinase subunit alpha; ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA; phosphoinositide-3-kinase catalytic alpha polypeptide; phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Chromosome
3
Map Location
3 B|3 15.7 cM
EC Number
2.7.1.153

Proteins

phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Refseq ID NP_032865
Protein GI 254540080
UniProt ID P42337
mRNA ID NM_008839
Length 1068
RefSeq Status VALIDATED
MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLVTIKHELFREARKYPLHQLLQDETSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFVIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPIDSFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHTGLSNRLARDNELRENDKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRQPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQHALN

Gene Information

Entrez Gene ID
18706
Gene Name
phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
Gene Symbol
Pik3ca
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0030027 IDA:MGI C lamellipodium
GO:0005942 IPI:MGI C phosphatidylinositol 3-kinase complex
GO:0005943 IEA:Ensembl C phosphatidylinositol 3-kinase complex, class IA
GO:0005886 IBA:RefGenome C plasma membrane
GO:0016303 IDA:MGI F 1-phosphatidylinositol-3-kinase activity
GO:0035005 IBA:RefGenome F 1-phosphatidylinositol-4-phosphate 3-kinase activity
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0043560 IPI:MGI F insulin receptor substrate binding
GO:0046934 IBA:RefGenome F phosphatidylinositol-4,5-bisphosphate 3-kinase activity
GO:0030295 IDA:BHF-UCL F protein kinase activator activity
GO:0004674 IEA:UniProtKB-KW F protein serine/threonine kinase activity
GO:0001525 IEA:UniProtKB-KW P angiogenesis
GO:0006006 IMP:MGI P glucose metabolic process
GO:0044029 IDA:BHF-UCL P hypomethylation of CpG island
GO:2000811 IEA:Ensembl P negative regulation of anoikis
GO:2000270 IDA:MGI P negative regulation of fibroblast apoptotic process
GO:0043524 IMP:MGI P negative regulation of neuron apoptotic process
GO:0036092 IDA:GOC P phosphatidylinositol-3-phosphate biosynthetic process
GO:0048015 IEA:InterPro P phosphatidylinositol-mediated signaling
GO:0033138 IDA:BHF-UCL P positive regulation of peptidyl-serine phosphorylation
GO:0045860 IDA:GOC P positive regulation of protein kinase activity
GO:0043491 IMP:MGI P protein kinase B signaling
GO:0006468 IDA:MGI P protein phosphorylation
GO:2000653 IDA:BHF-UCL P regulation of genetic imprinting
GO:0040014 IMP:MGI P regulation of multicellular organism growth

KEGG Pathway Links

KEGG Pathway ID Description
mmu04152 AMPK signaling pathway
mmu04024 cAMP signaling pathway
mmu04022 cGMP-PKG signaling pathway
mmu04550 Signaling pathways regulating pluripotency of stem cells

Domain Information

InterPro Annotations

Accession Description
IPR016024 Armadillo-type fold
IPR000008 C2 domain
IPR000403 Phosphatidylinositol 3-/4-kinase, catalytic domain
IPR018936 Phosphatidylinositol 3/4-kinase, conserved site
IPR003113 Phosphatidylinositol 3-kinase adaptor-binding (PI3K ABD) domain
IPR002420 Phosphatidylinositol 3-kinase, C2 domain
IPR000341 Phosphatidylinositol 3-kinase Ras-binding (PI3K RBD) domain
IPR015433 Phosphatidylinositol Kinase
IPR001263 Phosphoinositide 3-kinase, accessory (PIK) domain
IPR011009 Protein kinase-like domain
IPR029071 Ubiquitin-related domain

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
Protein Entry
PK3CA_MOUSE
UniProt ID
P42337
Species
Mouse

Comments

Comment Type Description
Catalytic Activity ATP + 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5- trisphosphate.
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Disruption Phenotype Lethal. Embryonic fibroblasts cells are resistant to oncogenic transformation induced by oncogenic receptor tyrosine kinases (RTKs), are unable to differentiate into adipocytes and deficient in cellular signaling in response to various growth factors. Defective responsiveness to insulin led to reduced somatic growth, hyperinsulinemia, glucose intolerance, hyperphagia and increased adiposity. {ECO:0000269|PubMed:16625210, ECO:0000269|PubMed:17060635, ECO:0000269|PubMed:17540175, ECO:0000269|PubMed:18449193}.
Domain The PI3K-ABD domain and the PI3K-RBD domain interact with the PI3K/PI4K kinase domain. The C2 PI3K-type domain may facilitate the recruitment to the plasma membrane. The inhibitory interactions with PIK3R1 are mediated by the PI3K-ABD domain and the C2 PI3K-type domain with the iSH2 (inter-SH2) region of PIK3R1, and the C2 PI3K-type domain, the PI3K helical domain, and the PI3K/PI4K kinase domain with the nSH2 (N-terminal SH2) region of PIK3R1. {ECO:0000250}.
Function Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. {ECO:0000269|PubMed:16625210, ECO:0000269|PubMed:16647110, ECO:0000269|PubMed:17060635, ECO:0000269|PubMed:17540175, ECO:0000269|PubMed:18449193, ECO:0000269|PubMed:21540297}.
Interaction P01112:HRAS (xeno); NbExp=2; IntAct=EBI-641748, EBI-350145; P26450:Pik3r1; NbExp=6; IntAct=EBI-641748, EBI-641764;
Similarity Belongs to the PI3/PI4-kinase family. {ECO:0000255|PROSITE-ProRule:PRU00877, ECO:0000255|PROSITE- ProRule:PRU00879, ECO:0000255|PROSITE-ProRule:PRU00880}.
Similarity Contains 1 C2 PI3K-type domain. {ECO:0000255|PROSITE- ProRule:PRU00880}.
Similarity Contains 1 PI3K-ABD domain. {ECO:0000255|PROSITE- ProRule:PRU00877}.
Similarity Contains 1 PI3K/PI4K domain. {ECO:0000255|PROSITE- ProRule:PRU00269}.
Similarity Contains 1 PI3K-RBD domain. {ECO:0000255|PROSITE- ProRule:PRU00879}.
Similarity Contains 1 PIK helical domain. {ECO:0000255|PROSITE- ProRule:PRU00878}.
Subunit Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS and KRAS. Interaction with HRAS/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP001328 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
254540080 RefSeq NP_032865 1068 phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Identical Sequences to LMP001328 proteins

Reference Database Accession Length Protein Name
GI:254540080 EMBL CDL93484.1 1068 unnamed protein product [Mus musculus]
GI:254540080 GenBank AAI30229.1 1068 Phosphatidylinositol 3-kinase, catalytic, alpha polypeptide [Mus musculus]
GI:254540080 GenBank EDL34990.1 1068 phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, isoform CRA_b [Mus musculus]
GI:254540080 RefSeq XP_006535472.1 1068 PREDICTED: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform isoform X1 [Mus musculus]
GI:254540080 RefSeq XP_006535473.1 1068 PREDICTED: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform isoform X2 [Mus musculus]
GI:254540080 SwissProt P42337.2 1068 RecName: Full=Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Short=PI3-kinase subunit alpha; Short=PI3K-alpha; Short=PI3Kalpha; Short=PtdIns-3-kinase subunit alpha; AltName: Full=Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Short=PtdIns-3-kinase subunit p110-alpha; Short=p110alpha; AltName: Full=Phosphoinositide-3-kinase catalytic alpha polypeptide; AltName: Full=Serine/threonine protein kinase PIK3CA [Mus musculus]

Related Sequences to LMP001328 proteins

Reference Database Accession Length Protein Name
GI:254540080 GenBank AAA18334.1 1068 phosphatidylinositol 3-kinase 110 kDa subunit [Mus musculus]
GI:254540080 GenBank AAQ80265.1 1068 Sequence 11 from patent US 6300111
GI:254540080 GenBank ABJ30013.1 1068 Sequence 10 from patent US 7094889
GI:254540080 GenBank AEC44978.1 1068 Sequence 107 from patent US 7863437
GI:254540080 GenBank AGN43281.1 1068 Sequence 107 from patent US 8450291
GI:254540080 RefSeq NP_596890.2 1068 phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform [Rattus norvegicus]