Gene/Proteome Database (LMPD)

LMPD ID
LMP001340
Gene ID
10026
Species
Homo sapiens (Human)
Gene Name
phosphatidylinositol glycan anchor biosynthesis, class K
Gene Symbol
PIGK
Synonyms
GPI8
Alternate Names
GPI-anchor transamidase; PIG-K; GPI8 homolog; GPI transamidase subunit; phosphatidylinositol glycan, class K; phosphatidylinositol-glycan biosynthesis class K protein
Chromosome
1
Map Location
1p31.1
EC Number
3.-.-.-
Summary
This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for PIGK

Proteins

GPI-anchor transamidase precursor
Refseq ID NP_005473
Protein GI 23199983
UniProt ID Q92643
mRNA ID NM_005482
Length 395
RefSeq Status REVIEWED
MAVTDSLSRAATVLATVLLLSFGSVAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIMESSYKEDQMDEKLMEPLKYAEQLPVAQIIHQKPKLKDWHPPGGFILGLWALIIMVFFKTYGIKHMKFIF
sig_peptide: 1..27 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2665 peptide sequence: MAVTDSLSRAATVLATVLLLSFGSVAA mat_peptide: 28..395 product: GPI-anchor transamidase calculated_mol_wt: 42605 peptide sequence: SHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIMESSYKEDQMDEKLMEPLKYAEQLPVAQIIHQKPKLKDWHPPGGFILGLWALIIMVFFKTYGIKHMKFIF

Gene Information

Entrez Gene ID
10026
Gene Name
phosphatidylinositol glycan anchor biosynthesis, class K
Gene Symbol
PIGK
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0042765 IMP:UniProtKB C GPI-anchor transamidase complex
GO:0005789 TAS:Reactome C endoplasmic reticulum membrane
GO:0030176 NAS:UniProtKB C integral component of endoplasmic reticulum membrane
GO:0016020 IDA:UniProtKB C membrane
GO:0003923 IMP:UniProtKB F GPI-anchor transamidase activity
GO:0004197 IEA:InterPro F cysteine-type endopeptidase activity
GO:0003756 TAS:UniProtKB F protein disulfide isomerase activity
GO:0006501 TAS:Reactome P C-terminal protein lipidation
GO:0016255 TAS:UniProtKB P attachment of GPI anchor to protein
GO:0044267 TAS:Reactome P cellular protein metabolic process
GO:0043687 TAS:Reactome P post-translational protein modification
GO:0006457 TAS:GOC P protein folding

KEGG Pathway Links

KEGG Pathway ID Description
hsa00563 Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
ko00563 Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
hsa01100 Metabolic pathways

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_1830 Attachment of GPI anchor to uPAR

Domain Information

InterPro Annotations

Accession Description
IPR028361 GPI-anchor transamidase
IPR001096 Peptidase C13, legumain

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol glycan anchor biosynthesis, class K
Protein Entry
GPI8_HUMAN
UniProt ID
Q92643
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q92643-1; Sequence=Displayed; Name=2; IsoId=Q92643-2; Sequence=VSP_056457; Note=No experimental confirmation available.;
Function Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.
Pathway Glycolipid biosynthesis; glycosylphosphatidylinositol- anchor biosynthesis.
Ptm The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.
Similarity Belongs to the peptidase C13 family.
Subcellular Location Endoplasmic reticulum membrane ; Single-pass type I membrane protein .
Subunit Forms a complex with PIGT, PIGS, PIGU and GAA1.

Identical and Related Proteins

Unique RefSeq proteins for LMP001340 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
23199983 RefSeq NP_005473 395 GPI-anchor transamidase precursor

Identical Sequences to LMP001340 proteins

Reference Database Accession Length Protein Name
GI:23199983 DBBJ BAG35850.1 395 unnamed protein product [Homo sapiens]
GI:23199983 GenBank EAX06380.1 395 phosphatidylinositol glycan, class K, isoform CRA_a [Homo sapiens]
GI:23199983 GenBank ADQ32189.1 395 phosphatidylinositol glycan anchor biosynthesis, class K, partial [synthetic construct]
GI:23199983 GenBank AHD71038.1 395 Sequence 4506 from patent US 8586006
GI:23199983 GenBank AIC50486.1 395 PIGK, partial [synthetic construct]
GI:23199983 SwissProt Q92643.2 395 RecName: Full=GPI-anchor transamidase; Short=GPI transamidase; AltName: Full=GPI8 homolog; Short=hGPI8; AltName: Full=Phosphatidylinositol-glycan biosynthesis class K protein; Short=PIG-K; Flags: Precursor [Homo sapiens]

Related Sequences to LMP001340 proteins

Reference Database Accession Length Protein Name
GI:23199983 GenBank JAA21868.1 395 phosphatidylinositol glycan anchor biosynthesis, class K [Pan troglodytes]
GI:23199983 GenBank JAA40418.1 395 phosphatidylinositol glycan anchor biosynthesis, class K [Pan troglodytes]
GI:23199983 RefSeq XP_001168438.1 395 PREDICTED: GPI-anchor transamidase [Pan troglodytes]
GI:23199983 RefSeq XP_003260272.1 395 PREDICTED: GPI-anchor transamidase [Nomascus leucogenys]
GI:23199983 RefSeq XP_003807837.1 395 PREDICTED: GPI-anchor transamidase [Pan paniscus]
GI:23199983 RefSeq XP_004026054.1 395 PREDICTED: GPI-anchor transamidase isoform 1 [Gorilla gorilla gorilla]