Gene/Proteome Database (LMPD)

LMPD ID
LMP001353
Gene ID
16330
Species
Mus musculus (Mouse)
Gene Name
inositol polyphosphate-5-phosphatase B
Gene Symbol
Inpp5b
Synonyms
5PTase; AW260155; INPP5P
Alternate Names
type II inositol 1,4,5-trisphosphate 5-phosphatase; phosphoinositide 5-phosphatase; inositol polyphosphate-5-phosphatase, 75 kDa
Chromosome
4
Map Location
4 D2|4 57.89 cM
EC Number
3.1.3.36
Summary
This gene encodes a member of the inositol polyphosphate-5-phosphatase (INPP5) family. This protein hydrolyzes the 5' phosphate from phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol-1,4,5-trisphosphate, which results in changes to multiple signaling pathways. This protein may be involved in protein trafficking and secretion. Homozygous knockout mice exhibit impaired spermatogenesis and male sterility. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Orthologs
View orthologs and multiple alignments for Inpp5b

Proteins

type II inositol 1,4,5-trisphosphate 5-phosphatase precursor
Refseq ID NP_032411
Protein GI 30231213
UniProt ID Q8K337
mRNA ID NM_008385
Length 993
RefSeq Status REVIEWED
MDQSVAIQETLVEGEYCVIAVQGVLCKGDSRQSRLLGLVRYRLENDAQEHALFLYTHRRMAITGDDVSLDQIVPLSKDFMLEEVSPDGELYILGSDVTVQLNTAELKLVFQLPFGSHTRTFLQEVARACPGFDPETRDPEFEWLSRHTCAEPDAESPKPREWNSDPGTRSGFAPIGGSRHQSRNARRGLEDVLPRGPGYILLWGGAAEEPEFLLAEEMHEGGPVRGRRPLAGRRDEALEEADWEMSAGGGSRERDCAGVSNVDSSRPNGRGPDQPSGARCPEKPENSLTRQNKSKSDMSEKVRSATVTVSDKAHILSVQKFGLRDTIVRSHLVQKEENYTYIQNFRFFVGTYNVNGQSPKECLRPWLSHSALAPDVYCVGFQELDLSKEAFFFHDTPKEEEWFKAVSESLHPDAKYAKVKFVRLVGIMLLLYVKQEHAAYISEVEAETVGTGIMGRMGNKGGVAIRFQLHNTSICVVNSHLAAHTEEYERRNQDYRDICSRMQFPQVDPSQPPLTINKHDVILWLGDLNYRIEELDVGKVKKLVEEKAFQTLYAHDQLKIQVAARTIFDGFTEGEITFQPTYKYDTGSDDWDTSEKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHKPVSSVFDIGVRVVNEELYRKTLEEIVRSLDKMENANIPSVTLSKREFCFENVKYMQLQTESFTIHNSQVPCQFEFINKPDEESYCKQWLTARPSKGFLLPDSHVEIELELFVNKSTATKLNSGKDTIEDILVLHLERGKDYFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLKTVSDLTLMSVQTADDRSQLENPMEIPKELWMMVDYLYRNAVQQEDLFQQPGLRPEFDHIRDCLDTGMIDQLCANNHSVAEALLLFLESLPEPVICYSAYHSCLECSGNYAASKQIILTLPSFHKNVFNYLMAFLQELLKNSANNHLDENILASIFGSLLLRNPARHQKLDMAEKKKAQEFIHQFLCGPL
mat_peptide: 1..990 product: Type II inositol 1,4,5-trisphosphate 5-phosphatase experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q8K337.1) calculated_mol_wt: 112495 peptide sequence: MDQSVAIQETLVEGEYCVIAVQGVLCKGDSRQSRLLGLVRYRLENDAQEHALFLYTHRRMAITGDDVSLDQIVPLSKDFMLEEVSPDGELYILGSDVTVQLNTAELKLVFQLPFGSHTRTFLQEVARACPGFDPETRDPEFEWLSRHTCAEPDAESPKPREWNSDPGTRSGFAPIGGSRHQSRNARRGLEDVLPRGPGYILLWGGAAEEPEFLLAEEMHEGGPVRGRRPLAGRRDEALEEADWEMSAGGGSRERDCAGVSNVDSSRPNGRGPDQPSGARCPEKPENSLTRQNKSKSDMSEKVRSATVTVSDKAHILSVQKFGLRDTIVRSHLVQKEENYTYIQNFRFFVGTYNVNGQSPKECLRPWLSHSALAPDVYCVGFQELDLSKEAFFFHDTPKEEEWFKAVSESLHPDAKYAKVKFVRLVGIMLLLYVKQEHAAYISEVEAETVGTGIMGRMGNKGGVAIRFQLHNTSICVVNSHLAAHTEEYERRNQDYRDICSRMQFPQVDPSQPPLTINKHDVILWLGDLNYRIEELDVGKVKKLVEEKAFQTLYAHDQLKIQVAARTIFDGFTEGEITFQPTYKYDTGSDDWDTSEKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHKPVSSVFDIGVRVVNEELYRKTLEEIVRSLDKMENANIPSVTLSKREFCFENVKYMQLQTESFTIHNSQVPCQFEFINKPDEESYCKQWLTARPSKGFLLPDSHVEIELELFVNKSTATKLNSGKDTIEDILVLHLERGKDYFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLKTVSDLTLMSVQTADDRSQLENPMEIPKELWMMVDYLYRNAVQQEDLFQQPGLRPEFDHIRDCLDTGMIDQLCANNHSVAEALLLFLESLPEPVICYSAYHSCLECSGNYAASKQIILTLPSFHKNVFNYLMAFLQELLKNSANNHLDENILASIFGSLLLRNPARHQKLDMAEKKKAQEFIHQFLC

Gene Information

Entrez Gene ID
16330
Gene Name
inositol polyphosphate-5-phosphatase B
Gene Symbol
Inpp5b
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0031410 IEA:UniProtKB-KW C cytoplasmic vesicle
GO:0005829 IDA:MGI C cytosol
GO:0005768 IEA:UniProtKB-KW C endosome
GO:0016021 IDA:MGI C integral component of membrane
GO:0015630 IEA:Ensembl C microtubule cytoskeleton
GO:0052658 IDA:UniProtKB F inositol-1,4,5-trisphosphate 5-phosphatase activity
GO:0004445 ISA:MGI F inositol-polyphosphate 5-phosphatase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0004439 IDA:UniProtKB F phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity
GO:0001701 IGI:MGI P in utero embryonic development
GO:0046856 IDA:UniProtKB P phosphatidylinositol dephosphorylation
GO:0070613 IMP:MGI P regulation of protein processing
GO:0007165 IEA:InterPro P signal transduction
GO:0030317 IMP:MGI P sperm motility
GO:0007283 IMP:MGI P spermatogenesis

KEGG Pathway Links

KEGG Pathway ID Description
mmu00562 Inositol phosphate metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
5893471 Rho GTPase cycle
5894027 Synthesis of IP2, IP, and Ins in the cytosol
5894025 Synthesis of IP3 and IP4 in the cytosol

Domain Information

InterPro Annotations

Accession Description
IPR005135 Endonuclease/exonuclease/phosphatase
IPR000300 Inositol polyphosphate-related phosphatase
IPR008936 Rho GTPase activation protein
IPR000198 Rho GTPase-activating protein domain

UniProt Annotations

Entry Information

Gene Name
inositol polyphosphate-5-phosphatase B
Protein Entry
I5P2_MOUSE
UniProt ID
Q8K337
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q8K337-1; Sequence=Displayed; Name=2; IsoId=Q8K337-2; Sequence=VSP_042226, VSP_042227; Name=3; IsoId=Q8K337-3; Sequence=VSP_042224, VSP_042225;
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate. {ECO:0000269|PubMed:9525932}.
Domain The ASH (ASPM-SPD2-Hydin) and RhoGAP (Rho GTPase activating) domains form a single folding module. The ASH domain has an immunoglobulin-like fold, the Rho-GAP domain lacks the catalytic arginine and is catalytically inactive. The ASH-RhoGAP module regulates the majority of the protein-protein interactions currently described. The ASH domain mediates association with membrane-targeting Rab GTPases. The Rho-GAP domain interacts with the endocytic adapter APPL1, which is then displaced by FAM109A and FAM109B as endosomes mature, all three interactions relie on F&H motifs, an approximately 12-13 amino-acid sequence centered around Phe and His residues essential for binding (By similarity). {ECO:0000250}.
Function Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events. {ECO:0000269|PubMed:11311145, ECO:0000269|PubMed:9525932}.
Ptm Isoprenylation at Cys-990 may be required for localization at the membrane. {ECO:0000250}.
Ptm May be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form. {ECO:0000250}.
Sequence Caution Sequence=AAB95412.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Similarity Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family. {ECO:0000305}.
Similarity Contains 1 PH domain. {ECO:0000305}.
Similarity Contains 1 Rho-GAP domain. {ECO:0000255|PROSITE- ProRule:PRU00172}.
Subcellular Location Cytoplasm, cytosol. Endoplasmic reticulum- Golgi intermediate compartment {ECO:0000250}. Early endosome membrane. Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, phagosome membrane. Golgi apparatus {ECO:0000250}.
Subunit Interacts with APPL1, FAM109A and FAM109B. Interacts with several Rab GTPases, at least RAB1A, RAB2A, RAB5A, RAB6A, RAB8A, RAB9A and RAB33B; these interactions may play a dual role in targeting INPP5B to the specific membranes and stimulating the phosphatase activity (By similarity). {ECO:0000250}.
Tissue Specificity Detected in kidney, liver, brain, lung and testis (at protein level). Detected in kidney and liver, and at lower levels in brain, lung and testis. {ECO:0000269|PubMed:9525932}.

Identical and Related Proteins

Unique RefSeq proteins for LMP001353 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30231213 RefSeq NP_032411 993 type II inositol 1,4,5-trisphosphate 5-phosphatase precursor

Identical Sequences to LMP001353 proteins

Reference Database Accession Length Protein Name
GI:30231213 GenBank AAH28864.1 993 Inositol polyphosphate-5-phosphatase B [Mus musculus]
GI:30231213 SwissProt Q8K337.1 993 RecName: Full=Type II inositol 1,4,5-trisphosphate 5-phosphatase; AltName: Full=Inositol polyphosphate-5-phosphatase B; AltName: Full=Phosphoinositide 5-phosphatase; Short=5PTase; Flags: Precursor [Mus musculus]

Related Sequences to LMP001353 proteins

Reference Database Accession Length Protein Name
GI:30231213 GenBank EDL30353.1 993 inositol polyphosphate-5-phosphatase B, isoform CRA_c [Mus musculus]
GI:30231213 RefSeq XP_006502867.1 994 PREDICTED: type II inositol 1,4,5-trisphosphate 5-phosphatase isoform X1 [Mus musculus]
GI:30231213 RefSeq XP_006502868.1 994 PREDICTED: type II inositol 1,4,5-trisphosphate 5-phosphatase isoform X2 [Mus musculus]
GI:30231213 RefSeq XP_006502869.1 916 PREDICTED: type II inositol 1,4,5-trisphosphate 5-phosphatase isoform X3 [Mus musculus]
GI:30231213 RefSeq XP_006980141.1 962 PREDICTED: LOW QUALITY PROTEIN: type II inositol 1,4,5-trisphosphate 5-phosphatase [Peromyscus maniculatus bairdii]
GI:30231213 RefSeq XP_008774314.1 997 PREDICTED: LOW QUALITY PROTEIN: type II inositol 1,4,5-trisphosphate 5-phosphatase-like isoform X1 [Rattus norvegicus]