Gene/Proteome Database (LMPD)

LMPD ID
LMP001475
Gene ID
6721
Species
Homo sapiens (Human)
Gene Name
sterol regulatory element binding transcription factor 2
Gene Symbol
SREBF2
Synonyms
SREBP-2; SREBP2; bHLHd2
Alternate Names
sterol regulatory element-binding protein 2; class D basic helix-loop-helix protein 2; sterol regulatory element-binding transcription factor 2
Chromosome
22
Map Location
22q13
Summary
This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Orthologs
View orthologs and multiple alignments for SREBF2

Proteins

sterol regulatory element-binding protein 2
Refseq ID NP_004590
Protein GI 27477113
UniProt ID Q12772
mRNA ID NM_004599
Length 1141
RefSeq Status REVIEWED
MDDSGELGGLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGSGGSGSSSGSSGSSSSSSNGRGSSSGAVDPSVQRSFTQVTLPSFSPSAASPQAPTLQVKVSPTSVPTTPRATPILQPRPQPQPQPQTQLQQQTVMITPTFSTTPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSSGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLKIEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTSLLQWGGAHDSDQHPHSGSGRSVLSFESGSGGWFDWMMPTLLLWLVNGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAGNLQTCLAVLGRALPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQCRRATPATEAGFEDEAKTSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTRCGGKLGFLASYFLSRAQSLCGPEHSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFTKVERIPKALEVTESPLVKAIFHACRAMHASLPGKADGQQSSFCHCERASGHLWSSLNVSGATSDPALNHVVQLLTCDLLLSLRTALWQKQASASQAVGETYHASGAELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTTQSTKHGEVDAWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCNDCQQMIVKLGGGTAIAAS
mat_peptide: 1..484 product: Processed sterol regulatory element-binding protein 2 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q12772.2) calculated_mol_wt: 51777 peptide sequence: MDDSGELGGLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGSGGSGSSSGSSGSSSSSSNGRGSSSGAVDPSVQRSFTQVTLPSFSPSAASPQAPTLQVKVSPTSVPTTPRATPILQPRPQPQPQPQTQLQQQTVMITPTFSTTPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSSGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLKIEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILL

Gene Information

Entrez Gene ID
6721
Gene Name
sterol regulatory element binding transcription factor 2
Gene Symbol
SREBF2
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000139 TAS:Reactome C Golgi membrane
GO:0032937 IDA:UniProtKB C SREBP-SCAP-Insig complex
GO:0005737 IDA:UniProtKB C cytoplasm
GO:0031410 IEA:UniProtKB-KW C cytoplasmic vesicle
GO:0005829 TAS:Reactome C cytosol
GO:0005783 IDA:UniProtKB C endoplasmic reticulum
GO:0005789 TAS:Reactome C endoplasmic reticulum membrane
GO:0005730 IDA:HPA C nucleolus
GO:0005654 TAS:Reactome C nucleoplasm
GO:0005634 IDA:UniProtKB C nucleus
GO:0070888 IDA:BHF-UCL F E-box binding
GO:0000978 IDA:BHF-UCL F RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0008022 IPI:UniProtKB F protein C-terminus binding
GO:0003700 IEA:Ensembl F sequence-specific DNA binding transcription factor activity
GO:0044255 TAS:Reactome P cellular lipid metabolic process
GO:0071499 NAS:BHF-UCL P cellular response to laminar fluid shear stress
GO:0008203 IEA:UniProtKB-KW P cholesterol metabolic process
GO:0006629 TAS:ProtInc P lipid metabolic process
GO:0090370 IDA:BHF-UCL P negative regulation of cholesterol efflux
GO:0000122 IDA:BHF-UCL P negative regulation of transcription from RNA polymerase II promoter
GO:0010886 IDA:BHF-UCL P positive regulation of cholesterol storage
GO:0045944 IDA:UniProtKB P positive regulation of transcription from RNA polymerase II promoter
GO:2000188 IEA:Ensembl P regulation of cholesterol homeostasis
GO:0072368 IDA:BHF-UCL P regulation of lipid transport by negative regulation of transcription from RNA polymerase II promoter
GO:0055098 IEP:BHF-UCL P response to low-density lipoprotein particle
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_147904 Activation of gene expression by SREBF (SREBP)

Domain Information

InterPro Annotations

Accession Description
IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain

UniProt Annotations

Entry Information

Gene Name
sterol regulatory element binding transcription factor 2
Protein Entry
SRBP2_HUMAN
UniProt ID
Q12772
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q12772-1; Sequence=Displayed; Name=2; IsoId=Q12772-2; Sequence=VSP_054283, VSP_054284, VSP_054285; Note=No experimental confirmation available.;
Function Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
Interaction P08047:SP1; NbExp=3; IntAct=EBI-465059, EBI-298336; P04637:TP53; NbExp=3; IntAct=EBI-465059, EBI-366083;
Ptm At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. {ECO
Ptm Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.
Sequence Caution Sequence=AAH51799.1; Type=Erroneous initiation; Evidence= ;
Similarity Belongs to the SREBP family.
Similarity Contains 1 bHLH (basic helix-loop-helix) domain.
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Subcellular Location Processed sterol regulatory element-binding protein 2: Nucleus.
Subunit Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139.
Tissue Specificity Ubiquitously expressed in adult and fetal tissues.

Identical and Related Proteins

Unique RefSeq proteins for LMP001475 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
27477113 RefSeq NP_004590 1141 sterol regulatory element-binding protein 2

Identical Sequences to LMP001475 proteins

Reference Database Accession Length Protein Name
GI:27477113 DBBJ BAG72804.1 1141 sterol regulatory element binding transcription factor 2, partial [synthetic construct]
GI:27477113 GenBank ACP57513.1 1141 Sequence 1642 from patent US 7482117
GI:27477113 GenBank ACP57514.1 1141 Sequence 1643 from patent US 7482117
GI:27477113 GenBank ADS31110.1 1141 Sequence 1642 from patent US 7781168
GI:27477113 GenBank ADS31111.1 1141 Sequence 1643 from patent US 7781168
GI:27477113 GenBank AHD75330.1 1141 Sequence 16609 from patent US 8586006

Related Sequences to LMP001475 proteins

Reference Database Accession Length Protein Name
GI:27477113 GenBank AAA50746.1 1141 sterol regulatory element binding protein-2 [Homo sapiens]
GI:27477113 GenBank AAB13894.1 1141 Sequence 54 from patent US 5527690
GI:27477113 GenBank AAE23378.1 1141 Sequence 54 from patent US 5891631
GI:27477113 GenBank AAH51799.1 1193 SREBF2 protein, partial [Homo sapiens]
GI:27477113 GenBank AFQ70483.1 1141 Sequence 25 from patent US 8227408
GI:27477113 GenBank JAA27356.1 1141 sterol regulatory element binding transcription factor 2 [Pan troglodytes]