Gene/Proteome Database (LMPD)

LMPD ID
LMP001480
Gene ID
5588
Species
Homo sapiens (Human)
Gene Name
protein kinase C, theta
Gene Symbol
PRKCQ
Synonyms
PRKCT; nPKC-theta
Alternate Names
protein kinase C theta type
Chromosome
10
Map Location
10p15
EC Number
2.7.11.13
Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for PRKCQ

Proteins

protein kinase C theta type isoform 1
Refseq ID NP_006248
Protein GI 5453976
UniProt ID Q04759
mRNA ID NM_006257
Length 706
RefSeq Status VALIDATED
MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDTEQIFREGPVEIGLPCSIKNEARPPCLPTPGKREPQGISWESPLDEVDKMCHLPEPELNKERPSLQIKLKIEDFILHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPFDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMERLIS
protein kinase C theta type isoform 2
Refseq ID NP_001229342
Protein GI 334848126
UniProt ID Q04759
mRNA ID NM_001242413
Length 643
RefSeq Status VALIDATED
MSPFLRIGLSNFDCGSCQSCQGEAVNPYCAVLVKEYVESENGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDTEQIFREGPVEIGLPCSIKNEARPPCLPTPGKREPQGISWESPLDEVDKMCHLPEPELNKERPSLQIKLKIEDFILHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPELFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPFDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMERLIS
protein kinase C theta type isoform 3
Refseq ID NP_001269573
Protein GI 544063437
UniProt ID Q04759
mRNA ID NM_001282644
Length 670
RefSeq Status VALIDATED
MSNQNGQMYIQKKPTMYPPWDSTFDAHINKGRVMQIIVKGKNVDLISETTVELYSLAERCRKNNGKTEIWLELKPQGRMLMNARYFLEMSDTKDMNEFETEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDTEQIFREGPVEIGLPCSIKNEARPPCLPTPGKREPQGISWESPLDEVDKMCHLPEPELNKERPSLQIKLKIEDFILHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPFDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMERLIS
protein kinase C theta type isoform 4
Refseq ID NP_001269574
Protein GI 544063391
UniProt ID Q04759
mRNA ID NM_001282645
Length 581
RefSeq Status VALIDATED
MDTKDMNEFETEGFFALHQRRGAIKQAKVHHVKCHEFTATFFPQPTFCSVCHEFVWGLNKQGYQCRQCNAAIHKKCIDKVIAKCTGSAINSRETMFHKERFKIDMPHRFKVYNYKSPTFCEHCGTLLWGLARQGLKCDACGMNVHHRCQTKVANLCGINQKLMAEALAMIESTQQARCLRDTEQIFREGPVEIGLPCSIKNEARPPCLPTPGKREPQGISWESPLDEVDKMCHLPEPELNKERPSLQIKLKIEDFILHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHPFLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHSVDWWSFGVLLYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPEKRLGVRGDIRQHPLFREINWEELERKEIDPPFRPKVKSPFDCSNFDKEFLNEKPRLSFADRALINSMDQNMFRNFSFMNPGMERLIS

Gene Information

Entrez Gene ID
5588
Gene Name
protein kinase C, theta
Gene Symbol
PRKCQ
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 TAS:Reactome C cytosol
GO:0001772 IEA:Ensembl C immunological synapse
GO:0005886 TAS:Reactome C plasma membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0004697 IEA:UniProtKB-EC F protein kinase C activity
GO:0004674 NAS:UniProtKB F protein serine/threonine kinase activity
GO:0004842 TAS:Reactome F ubiquitin-protein transferase activity
GO:0038095 TAS:Reactome P Fc-epsilon receptor signaling pathway
GO:0050852 TAS:Reactome P T cell receptor signaling pathway
GO:0006915 TAS:Reactome P apoptotic process
GO:0007411 TAS:Reactome P axon guidance
GO:0007596 TAS:Reactome P blood coagulation
GO:0006921 TAS:Reactome P cellular component disassembly involved in execution phase of apoptosis
GO:0006954 IEA:UniProtKB-KW P inflammatory response
GO:0045087 TAS:Reactome P innate immune response
GO:0035556 NAS:UniProtKB P intracellular signal transduction
GO:0006509 ISS:BHF-UCL P membrane protein ectodomain proteolysis
GO:0070233 IMP:UniProtKB P negative regulation of T cell apoptotic process
GO:0046627 IMP:UniProtKB P negative regulation of insulin receptor signaling pathway
GO:0007603 TAS:Reactome P phototransduction, visible light
GO:0030168 TAS:Reactome P platelet activation
GO:0051092 IDA:UniProtKB P positive regulation of NF-kappaB transcription factor activity
GO:0050870 ISS:UniProtKB P positive regulation of T cell activation
GO:0042102 IEA:Ensembl P positive regulation of T cell proliferation
GO:2000318 ISS:UniProtKB P positive regulation of T-helper 17 type immune response
GO:2000570 ISS:UniProtKB P positive regulation of T-helper 2 cell activation
GO:0032740 ISS:UniProtKB P positive regulation of interleukin-17 production
GO:0045086 IEA:Ensembl P positive regulation of interleukin-2 biosynthetic process
GO:0032753 ISS:UniProtKB P positive regulation of interleukin-4 production
GO:0016567 TAS:GOC P protein ubiquitination
GO:0001558 NAS:UniProtKB P regulation of cell growth
GO:0090330 ISS:UniProtKB P regulation of platelet aggregation
GO:0022400 TAS:Reactome P regulation of rhodopsin mediated signaling pathway
GO:0006355 ISS:UniProtKB P regulation of transcription, DNA-templated
GO:0016056 TAS:Reactome P rhodopsin mediated signaling pathway

KEGG Pathway Links

KEGG Pathway ID Description
hsa04750 Inflammatory mediator regulation of TRP channels
hsa05162 Measles
hsa04064 NF-kappa B signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR000961 AGC-kinase, C-terminal
IPR000008 C2 domain
IPR020454 Diacylglycerol/phorbol-ester binding
IPR014376 Protein kinase C, delta/epsilon/eta/theta types
IPR027264 Protein kinase C, theta
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR000719 Protein kinase domain
IPR017441 Protein kinase, ATP binding site
IPR017892 Protein kinase, C-terminal
IPR011009 Protein kinase-like domain
IPR008271 Serine/threonine-protein kinase, active site
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
protein kinase C, theta
Protein Entry
KPCT_HUMAN
UniProt ID
Q04759
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q04759-1; Sequence=Displayed; Name=2; IsoId=Q04759-2; Sequence=VSP_017294; Name=3; IsoId=Q04759-3; Sequence=VSP_054550; Note=No experimental confirmation available.;
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Cofactor Name=Mg(2+); Xref=ChEBI
Domain The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
Enzyme Regulation Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.
Function Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non- redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non- canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1. {ECO
Interaction Q06187:BTK; NbExp=2; IntAct=EBI-374762, EBI-624835; P06241:FYN; NbExp=7; IntAct=EBI-374762, EBI-515315; O76003:GLRX3; NbExp=5; IntAct=EBI-374762, EBI-374781; Q00613:HSF1; NbExp=2; IntAct=EBI-374762, EBI-719620; P35570:Irs1 (xeno); NbExp=2; IntAct=EBI-374762, EBI-520230; P06239:LCK; NbExp=2; IntAct=EBI-374762, EBI-1348; Q8IVH8:MAP4K3; NbExp=4; IntAct=EBI-374762, EBI-1758170; Q05513:PRKCZ; NbExp=3; IntAct=EBI-374762, EBI-295351;
Ptm Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PRKCQ upon antigen receptor ligation. Following TCR stimulation, phosphorylated at Tyr-90 and Ser-685. {ECO
Similarity Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Similarity Contains 1 AGC-kinase C-terminal domain.
Similarity Contains 1 C2 domain.
Similarity Contains 1 protein kinase domain.
Similarity Contains 2 phorbol-ester/DAG-type zinc fingers.
Subcellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.
Subunit Interacts with GLRX3 (via N-terminus). Interacts with ECT2. Part of a lipid raft complex composed at least of BCL10, CARD11, MALT1 and IKBKB. {ECO
Tissue Specificity Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.

Identical and Related Proteins

Unique RefSeq proteins for LMP001480 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
5453976 RefSeq NP_006248 706 protein kinase C theta type isoform 1
334848126 RefSeq NP_001229342 643 protein kinase C theta type isoform 2
544063437 RefSeq NP_001269573 670 protein kinase C theta type isoform 3
544063391 RefSeq NP_001269574 581 protein kinase C theta type isoform 4

Identical Sequences to LMP001480 proteins

Reference Database Accession Length Protein Name
GI:544063391 DBBJ BAG57313.1 581 unnamed protein product [Homo sapiens]
GI:5453976 GenBank ADS73646.1 706 Sequence 115 from patent US 7811794
GI:5453976 GenBank AED17332.1 706 Sequence 2450 from patent US 7879556
GI:5453976 GenBank AED47659.1 706 Sequence 2450 from patent US 7892745
GI:5453976 GenBank AFO05898.1 706 Sequence 536 from patent US 8216786
GI:5453976 GenBank AHD70224.1 706 Sequence 2313 from patent US 8586006
GI:5453976 RefSeq XP_006717528.1 706 PREDICTED: protein kinase C theta type isoform X3 [Homo sapiens]

Related Sequences to LMP001480 proteins

Reference Database Accession Length Protein Name
GI:544063437 GenBank AAA60101.1 706 protein kinase C-theta [Homo sapiens]
GI:544063391 GenBank AAA60101.1 706 protein kinase C-theta [Homo sapiens]
GI:334848126 GenBank AAU29340.1 643 protein kinase C theta [Homo sapiens]
GI:544063437 GenBank AAI13360.1 706 Protein kinase C, theta [Homo sapiens]
GI:544063391 GenBank AAI13360.1 706 Protein kinase C, theta [Homo sapiens]
GI:5453976 GenBank ABW49137.1 706 Sequence 171 from patent US 7265092
GI:544063391 GenBank ABY15080.1 706 Sequence 455 from patent US 7306913
GI:544063437 GenBank ABY15080.1 706 Sequence 455 from patent US 7306913
GI:544063437 GenBank ABY15082.1 706 Sequence 457 from patent US 7306913
GI:544063391 GenBank ABY15082.1 706 Sequence 457 from patent US 7306913
GI:5453976 GenBank ADM03576.1 706 Sequence 171 from patent US 7727958
GI:544063437 GenBank AED17332.1 706 Sequence 2450 from patent US 7879556
GI:544063391 GenBank AED17332.1 706 Sequence 2450 from patent US 7879556
GI:544063391 GenBank AED47659.1 706 Sequence 2450 from patent US 7892745
GI:544063437 GenBank AED47659.1 706 Sequence 2450 from patent US 7892745
GI:5453976 GenBank AGV86234.1 706 Sequence 171 from patent US 8524673
GI:5453976 GenBank AIC54949.1 706 PRKCQ, partial [synthetic construct]
GI:334848126 RefSeq XP_002820551.1 643 PREDICTED: protein kinase C theta type isoform X2 [Pongo abelii]
GI:334848126 RefSeq XP_004758003.1 643 PREDICTED: protein kinase C theta type isoform X4 [Mustela putorius furo]
GI:334848126 RefSeq XP_004806657.1 643 PREDICTED: protein kinase C theta type isoform X4 [Mustela putorius furo]
GI:5453976 RefSeq XP_005252553.1 740 PREDICTED: protein kinase C theta type isoform X1 [Homo sapiens]
GI:334848126 RefSeq XP_005320355.1 643 PREDICTED: protein kinase C theta type isoform X2 [Ictidomys tridecemlineatus]
GI:334848126 RefSeq XP_005564638.1 643 PREDICTED: protein kinase C theta type isoform X3 [Macaca fascicularis]
GI:5453976 RefSeq XP_008951372.1 706 PREDICTED: protein kinase C theta type isoform X2 [Pan paniscus]