Gene/Proteome Database (LMPD)

LMPD ID
LMP001597
Gene ID
11686
Species
Mus musculus (Mouse)
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
Alox12b
Synonyms
Aloxe2; e-LOX2
Alternate Names
arachidonate 12-lipoxygenase, 12R-type; 12R-LOX; e-LOX 2; 12R-lipoxygenase; epidermis-type lipoxygenase 2; epidermis-type lipoxygenase 12
Chromosome
11
Map Location
11 B3|11 42.38 cM
EC Number
1.13.11.-

Proteins

arachidonate 12-lipoxygenase, 12R-type
Refseq ID NP_033789
Protein GI 6753040
UniProt ID O70582
mRNA ID NM_009659
Length 701
RefSeq Status PROVISIONAL
MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLHKEPHSFLAKDPWYCNYVQICAPDCRVYHFPAYQWMDGYETLALREATGKITADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWDGYIPGFPILINIKATRFLNSNLRFSFVKTASFFYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKSVVSEYVAEHWTEDSFFGYQYLNGINPGLIRRCTQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIPTVELNGQQQHHCAPMCLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLGRESSGFPTCLRTIPELIEYVTMVMYTCSARHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMDTLPDVKTTCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIRQRNKCLTLPYYYLDPVLIENSISI

Gene Information

Entrez Gene ID
11686
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
Alox12b
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0004052 IDA:UniProtKB F arachidonate 12-lipoxygenase activity
GO:0003824 IDA:MGI F catalytic activity
GO:0005506 IEA:InterPro F iron ion binding
GO:1990136 IDA:UniProtKB F linoleate 9S-lipoxygenase activity
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0046513 IMP:UniProtKB P ceramide biosynthetic process
GO:0061436 IMP:UniProtKB P establishment of skin barrier
GO:0051122 ISS:UniProtKB P hepoxilin biosynthetic process
GO:0043651 IDA:UniProtKB P linoleic acid metabolic process
GO:0019372 ISS:UniProtKB P lipoxygenase pathway
GO:0055114 ISS:UniProtKB P oxidation-reduction process
GO:0043410 ISS:UniProtKB P positive regulation of MAPK cascade
GO:0010628 ISS:UniProtKB P positive regulation of gene expression
GO:0070257 ISS:UniProtKB P positive regulation of mucus secretion
GO:0006497 IMP:UniProtKB P protein lipidation
GO:0006665 ISS:UniProtKB P sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
mmu00590 Arachidonic acid metabolism
mmu01100 Metabolic pathways
mmu04726 Serotonergic synapse

REACTOME Pathway Links

REACTOME Pathway ID Description
5894068 Synthesis of 12-eicosatetraenoic acid derivatives

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 12-lipoxygenase, 12R type
Protein Entry
LX12B_MOUSE
UniProt ID
O70582
Species
Mouse

Comments

Comment Type Description
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12R)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate. {ECO:0000269|PubMed:16129665}.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|PROSITE-ProRule:PRU00726}; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726};
Developmental Stage In the embryo, expression begins at day 15.5. {ECO:0000269|PubMed:9837935}.
Disruption Phenotype Mice die within 3 to 5 hours after birth due to defective skin barrier function loosing around 30% of body weight within 3 hours. Dehydration through the skin is increased 8 folds. The outside-in barrier acquisition is also affected, the skin remaining permeable at E18.5 while it is impermeable in wild- type mice. The stratum corneum is more tightly packed while other layers are unaffected. Processing of filaggrin/FG is aberrant and the skin displays structural abnormalities. The cornified envelope is more fragile and the ceramide composition of the epidermis is altered. {ECO:0000269|PubMed:17403930, ECO:0000269|PubMed:21558561}.
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins. {ECO:0000269|PubMed:16129665, ECO:0000269|PubMed:17403930, ECO:0000269|PubMed:17429434, ECO:0000269|PubMed:21558561}.
Miscellaneous Mummy, a recessive ethylnitrosurea-induced mutant has a nonsense mutation in the catalytic domain of Lox12b, resulting in truncation of the protein by 68 amino acids. The affected mice are born with red, shiny skin that dessicates and appears scaly. They probably die of dehydration like mice with targeted disruption of the gene (PubMed:17429434). {ECO:0000305|PubMed:17429434}.
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Pathway Lipid metabolism; sphingolipid metabolism.
Similarity Belongs to the lipoxygenase family. {ECO:0000305}.
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Subcellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00726}.
Tissue Specificity Expressed in skin epidermis and other stratified epithelia including tongue and forestomach. Low levels of expression are found in trachea, brain and lung. Not expressed in intestine, liver, kidney, adipose tissue, muscle or hematopoietic cells. {ECO:0000269|PubMed:9518531}.

Identical and Related Proteins

Unique RefSeq proteins for LMP001597 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6753040 RefSeq NP_033789 701 arachidonate 12-lipoxygenase, 12R-type

Identical Sequences to LMP001597 proteins

Reference Database Accession Length Protein Name
GI:6753040 EMBL CAA74714.1 701 arachidonate 12-lipoxygenase [Mus musculus]
GI:6753040 GenBank AAI13150.1 701 Arachidonate 12-lipoxygenase, 12R type [Mus musculus]
GI:6753040 SwissProt O70582.1 701 RecName: Full=Arachidonate 12-lipoxygenase, 12R-type; Short=12R-LOX; Short=12R-lipoxygenase; AltName: Full=Epidermis-type lipoxygenase 12; AltName: Full=Epidermis-type lipoxygenase 2; Short=e-LOX 2 [Mus musculus]

Related Sequences to LMP001597 proteins

Reference Database Accession Length Protein Name
GI:6753040 GenBank AAC79681.1 701 lipoxygenase [Mus musculus]
GI:6753040 GenBank AAX85361.1 701 arachidonate 12-lipoxygenase 12R type [Rattus norvegicus]
GI:6753040 GenBank AAX85362.1 701 arachidonate 12-lipoxygenase 12R type [Rattus norvegicus]
GI:6753040 GenBank AAX85363.1 701 arachidonate 12-lipoxygenase 12R type [Rattus norvegicus]
GI:6753040 RefSeq NP_001034466.1 701 arachidonate 12-lipoxygenase, 12R-type [Rattus norvegicus]
GI:6753040 SwissProt Q2KMM4.1 701 RecName: Full=Arachidonate 12-lipoxygenase, 12R-type; Short=12R-LOX; Short=12R-lipoxygenase; AltName: Full=Epidermis-type lipoxygenase 12 [Rattus norvegicus]