Gene/Proteome Database (LMPD)
LMPD ID
LMP001597
Gene ID
Species
Mus musculus (Mouse)
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
Synonyms
Aloxe2; e-LOX2
Alternate Names
arachidonate 12-lipoxygenase, 12R-type; 12R-LOX; e-LOX 2; 12R-lipoxygenase; epidermis-type lipoxygenase 2; epidermis-type lipoxygenase 12
Chromosome
11
Map Location
11 B3|11 42.38 cM
EC Number
1.13.11.-
Proteins
| arachidonate 12-lipoxygenase, 12R-type | |
|---|---|
| Refseq ID | NP_033789 |
| Protein GI | 6753040 |
| UniProt ID | O70582 |
| mRNA ID | NM_009659 |
| Length | 701 |
| RefSeq Status | PROVISIONAL |
| MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLHKEPHSFLAKDPWYCNYVQICAPDCRVYHFPAYQWMDGYETLALREATGKITADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWDGYIPGFPILINIKATRFLNSNLRFSFVKTASFFYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKSVVSEYVAEHWTEDSFFGYQYLNGINPGLIRRCTQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIPTVELNGQQQHHCAPMCLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLGRESSGFPTCLRTIPELIEYVTMVMYTCSARHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMDTLPDVKTTCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIRQRNKCLTLPYYYLDPVLIENSISI | |
Gene Information
Entrez Gene ID
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
Species
Mus musculus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IEA:UniProtKB-KW | C | cytoplasm |
| GO:0004052 | IDA:UniProtKB | F | arachidonate 12-lipoxygenase activity |
| GO:0003824 | IDA:MGI | F | catalytic activity |
| GO:0005506 | IEA:InterPro | F | iron ion binding |
| GO:1990136 | IDA:UniProtKB | F | linoleate 9S-lipoxygenase activity |
| GO:0019369 | IDA:UniProtKB | P | arachidonic acid metabolic process |
| GO:0046513 | IMP:UniProtKB | P | ceramide biosynthetic process |
| GO:0061436 | IMP:UniProtKB | P | establishment of skin barrier |
| GO:0051122 | ISS:UniProtKB | P | hepoxilin biosynthetic process |
| GO:0043651 | IDA:UniProtKB | P | linoleic acid metabolic process |
| GO:0019372 | ISS:UniProtKB | P | lipoxygenase pathway |
| GO:0055114 | ISS:UniProtKB | P | oxidation-reduction process |
| GO:0010628 | ISS:UniProtKB | P | positive regulation of gene expression |
| GO:0043410 | ISS:UniProtKB | P | positive regulation of MAPK cascade |
| GO:0070257 | ISS:UniProtKB | P | positive regulation of mucus secretion |
| GO:0006497 | IMP:UniProtKB | P | protein lipidation |
| GO:0006665 | ISS:UniProtKB | P | sphingolipid metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| mmu00590 | Arachidonic acid metabolism |
| mmu01100 | Metabolic pathways |
| mmu04726 | Serotonergic synapse |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 5894068 | Synthesis of 12-eicosatetraenoic acid derivatives |
Domain Information
UniProt Annotations
Entry Information
Gene Name
arachidonate 12-lipoxygenase, 12R type
Protein Entry
LX12B_MOUSE
UniProt ID
Species
Mouse
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12R)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate. {ECO:0000269|PubMed:16129665}. |
| Cofactor | Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|PROSITE-ProRule:PRU00726}; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726}; |
| Developmental Stage | In the embryo, expression begins at day 15.5. {ECO:0000269|PubMed:9837935}. |
| Disruption Phenotype | Mice die within 3 to 5 hours after birth due to defective skin barrier function loosing around 30% of body weight within 3 hours. Dehydration through the skin is increased 8 folds. The outside-in barrier acquisition is also affected, the skin remaining permeable at E18.5 while it is impermeable in wild- type mice. The stratum corneum is more tightly packed while other layers are unaffected. Processing of filaggrin/FG is aberrant and the skin displays structural abnormalities. The cornified envelope is more fragile and the ceramide composition of the epidermis is altered. {ECO:0000269|PubMed:17403930, ECO:0000269|PubMed:21558561}. |
| Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins. {ECO:0000269|PubMed:16129665, ECO:0000269|PubMed:17403930, ECO:0000269|PubMed:17429434, ECO:0000269|PubMed:21558561}. |
| Miscellaneous | Mummy, a recessive ethylnitrosurea-induced mutant has a nonsense mutation in the catalytic domain of Lox12b, resulting in truncation of the protein by 68 amino acids. The affected mice are born with red, shiny skin that dessicates and appears scaly. They probably die of dehydration like mice with targeted disruption of the gene (PubMed:17429434). {ECO:0000305|PubMed:17429434}. |
| Pathway | Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis. |
| Pathway | Lipid metabolism; sphingolipid metabolism. |
| Similarity | Belongs to the lipoxygenase family. {ECO:0000305}. |
| Similarity | Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}. |
| Similarity | Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}. |
| Subcellular Location | Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00726}. |
| Tissue Specificity | Expressed in skin epidermis and other stratified epithelia including tongue and forestomach. Low levels of expression are found in trachea, brain and lung. Not expressed in intestine, liver, kidney, adipose tissue, muscle or hematopoietic cells. {ECO:0000269|PubMed:9518531}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP001597 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 6753040 | RefSeq | NP_033789 | 701 | arachidonate 12-lipoxygenase, 12R-type |
Identical Sequences to LMP001597 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6753040 | EMBL | CAA74714.1 | 701 | arachidonate 12-lipoxygenase [Mus musculus] |
| GI:6753040 | GenBank | AAI13150.1 | 701 | Arachidonate 12-lipoxygenase, 12R type [Mus musculus] |
| GI:6753040 | SwissProt | O70582.1 | 701 | RecName: Full=Arachidonate 12-lipoxygenase, 12R-type; Short=12R-LOX; Short=12R-lipoxygenase; AltName: Full=Epidermis-type lipoxygenase 12; AltName: Full=Epidermis-type lipoxygenase 2; Short=e-LOX 2 [Mus musculus] |
Related Sequences to LMP001597 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6753040 | GenBank | AAC79681.1 | 701 | lipoxygenase [Mus musculus] |
| GI:6753040 | GenBank | AAX85361.1 | 701 | arachidonate 12-lipoxygenase 12R type [Rattus norvegicus] |
| GI:6753040 | GenBank | AAX85362.1 | 701 | arachidonate 12-lipoxygenase 12R type [Rattus norvegicus] |
| GI:6753040 | GenBank | AAX85363.1 | 701 | arachidonate 12-lipoxygenase 12R type [Rattus norvegicus] |
| GI:6753040 | RefSeq | NP_001034466.1 | 701 | arachidonate 12-lipoxygenase, 12R-type [Rattus norvegicus] |
| GI:6753040 | SwissProt | Q2KMM4.1 | 701 | RecName: Full=Arachidonate 12-lipoxygenase, 12R-type; Short=12R-LOX; Short=12R-lipoxygenase; AltName: Full=Epidermis-type lipoxygenase 12 [Rattus norvegicus] |