Gene/Proteome Database (LMPD)

LMPD ID
LMP001611
Gene ID
11980
Species
Mus musculus (Mouse)
Gene Name
ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Gene Symbol
Atp8a1
Synonyms
AI481521; AI853962; APLT; AW743152; AW822227; Atp3a2; B230107D19Rik; ClassI
Alternate Names
phospholipid-transporting ATPase IA; ATPase 8A1, p type; chromaffin granule ATPase II; ATPase class I type 8A member 1; probable phospholipid-transporting ATPase IA; P4-ATPase flippase complex alpha subunit ATP8A1; ATPase 8A1, aminophospholipid transporter (APLT), class I
Chromosome
5
Map Location
5 C3.1|5
EC Number
3.6.3.1

Proteins

phospholipid-transporting ATPase IA isoform a
Refseq ID NP_001034088
Protein GI 84781802
UniProt ID P70704
mRNA ID NM_001038999
Length 1164
RefSeq Status VALIDATED
MPTMRRTVSEIRSRAEGYEKTDDVSEKTSLADQEEVRTIFINQPQLTKFCNNHVSTAKYNVITFLPRFLYSQFRRAANSFFLFIALLQQIPDVSPTGRYTTLVPLLFILAVAAIKEIIEDIKRHKADNAVNKKQTQVLRNGAWEIVHWEKVAVGEIVKVTNGEHLPADLLSLSSSEPQAMCYIETSNLDGETNLKIRQGLPATSDIKDIDSLMRISGRIECESPNRHLYDFVGNIRLDGHGTVPLGADQILLRGAQLRNTQWVHGIVVYTGHDTKLMQNSTSPPLKLSNVERITNVQILILFCILIAMSLVCSVGSAIWNRRHSGKDWYLHLHYGGASNFGLNFLTFIILFNNLIPISLLVTLEVVKFTQAYFINWDLDMHYEPTDTAAMARTSNLNEELGQVKYIFSDKTGTLTCNVMQFKKCTIAGVAYGHVPEPEDYGCSPDEWQSSQFGDEKTFNDPSLLDNLQNNHPTAPIICEFLTMMAVCHTAVPEREGDKIIYQAASPDEGALVRAAKQLNFVFTGRTPDSVIIDSLGQEERYELLNVLEFTSARKRMSVVVRTPSGKLRLYCKGADTVIYERLAETSKYKEITLKHLEQFATEGLRTLCFAVAEISESDFEEWRAVYHRASTSVQNRLLKLEESYELIEKNLQLLGATAIEDKLQDQVPETIETLMKADIKIWILTGDKQETAINIGHSCRLLKRNMGMIVINEGSLDGTRETLSRHCTTLGDALRKENDFALIIDGKTLKYALTFGVRQYFLDLALSCKAVICCRVSPLQKSEVVEMVKKQVKVITLAIGDGANDVSMIQTAHVGVGISGNEGLQAANSSDYSIAQFKYLKNLLMVHGAWNYNRVSKCILYCFYKNIVLYIIEIWFAFVNGFSGQILFERWCIGLYNVMFTAMPPLTLGIFERSCRKENMLKYPELYKTSQNALDFNTKVFWVHCLNGLFHSVILFWFPLKALQYGTVFGNGKTSDYLLLGNFVYTFVVITVCLKAGLETSYWTWFSHIAIWGSIALWVVFFGIYSSLWPAVPMAPDMSGEAAMLFSSGVFWVGLLSIPVASLLLDVLYKVIKRTAFKTLVDEVQELEAKSQDPGAVVLGKSLTERAQLLKNVFKKNHVNLYRSESLQQNLLHGYAFSQDENGIVSQSEVIRAYDTTKQRPDEW
phospholipid-transporting ATPase IA isoform b
Refseq ID NP_033857
Protein GI 7106257
UniProt ID P70704
mRNA ID NM_009727
Length 1149
RefSeq Status VALIDATED
MPTMRRTVSEIRSRAEGYEKTDDVSEKTSLADQEEVRTIFINQPQLTKFCNNHVSTAKYNVITFLPRFLYSQFRRAANSFFLFIALLQQIPDVSPTGRYTTLVPLLFILAVAAIKEIIEDIKRHKADNAVNKKQTQVLRNGAWEIVHWEKVNVGDIVIIKGKEYIPADTVLLSSSEPQAMCYIETSNLDGETNLKIRQGLPATSDIKDIDSLMRISGRIECESPNRHLYDFVGNIRLDGHGTVPLGADQILLRGAQLRNTQWVHGIVVYTGHDTKLMQNSTSPPLKLSNVERITNVQILILFCILIAMSLVCSVGSAIWNRRHSGKDWYLHLHYGGASNFGLNFLTFIILFNNLIPISLLVTLEVVKFTQAYFINWDLDMHYEPTDTAAMARTSNLNEELGQVKYIFSDKTGTLTCNVMQFKKCTIAGVAYGQSSQFGDEKTFNDPSLLDNLQNNHPTAPIICEFLTMMAVCHTAVPEREGDKIIYQAASPDEGALVRAAKQLNFVFTGRTPDSVIIDSLGQEERYELLNVLEFTSARKRMSVVVRTPSGKLRLYCKGADTVIYERLAETSKYKEITLKHLEQFATEGLRTLCFAVAEISESDFEEWRAVYHRASTSVQNRLLKLEESYELIEKNLQLLGATAIEDKLQDQVPETIETLMKADIKIWILTGDKQETAINIGHSCRLLKRNMGMIVINEGSLDGTRETLSRHCTTLGDALRKENDFALIIDGKTLKYALTFGVRQYFLDLALSCKAVICCRVSPLQKSEVVEMVKKQVKVITLAIGDGANDVSMIQTAHVGVGISGNEGLQAANSSDYSIAQFKYLKNLLMVHGAWNYNRVSKCILYCFYKNIVLYIIEIWFAFVNGFSGQILFERWCIGLYNVMFTAMPPLTLGIFERSCRKENMLKYPELYKTSQNALDFNTKVFWVHCLNGLFHSVILFWFPLKALQYGTVFGNGKTSDYLLLGNFVYTFVVITVCLKAGLETSYWTWFSHIAIWGSIALWVVFFGIYSSLWPAVPMAPDMSGEAAMLFSSGVFWVGLLSIPVASLLLDVLYKVIKRTAFKTLVDEVQELEAKSQDPGAVVLGKSLTERAQLLKNVFKKNHVNLYRSESLQQNLLHGYAFSQDENGIVSQSEVIRAYDTTKQRPDEW
phospholipid-transporting ATPase IA isoform c
Refseq ID NP_001271274
Protein GI 547234934
UniProt ID P70704
mRNA ID NM_001284345
Length 1164
RefSeq Status VALIDATED
MPTMRRTVSEIRSRAEGYEKTDDVSEKTSLADQEEVRTIFINQPQLTKFCNNHVSTAKYNVITFLPRFLYSQFRRAANSFFLFIALLQQIPDVSPTGRYTTLVPLLFILAVAAIKEIIEDIKRHKADNAVNKKQTQVLRNGAWEIVHWEKVNVGDIVIIKGKEYIPADTVLLSSSEPQAMCYIETSNLDGETNLKIRQGLPATSDIKDIDSLMRISGRIECESPNRHLYDFVGNIRLDGHGTVPLGADQILLRGAQLRNTQWVHGIVVYTGHDTKLMQNSTSPPLKLSNVERITNVQILILFCILIAMSLVCSVGSAIWNRRHSGKDWYLHLHYGGASNFGLNFLTFIILFNNLIPISLLVTLEVVKFTQAYFINWDLDMHYEPTDTAAMARTSNLNEELGQVKYIFSDKTGTLTCNVMQFKKCTIAGVAYGHVPEPEDYGCSPDEWQSSQFGDEKTFNDPSLLDNLQNNHPTAPIICEFLTMMAVCHTAVPEREGDKIIYQAASPDEGALVRAAKQLNFVFTGRTPDSVIIDSLGQEERYELLNVLEFTSARKRMSVVVRTPSGKLRLYCKGADTVIYERLAETSKYKEITLKHLEQFATEGLRTLCFAVAEISESDFEEWRAVYHRASTSVQNRLLKLEESYELIEKNLQLLGATAIEDKLQDQVPETIETLMKADIKIWILTGDKQETAINIGHSCRLLKRNMGMIVINEGSLDGTRETLSRHCTTLGDALRKENDFALIIDGKTLKYALTFGVRQYFLDLALSCKAVICCRVSPLQKSEVVEMVKKQVKVITLAIGDGANDVSMIQTAHVGVGISGNEGLQAANSSDYSIAQFKYLKNLLMVHGAWNYNRVSKCILYCFYKNIVLYIIEIWFAFVNGFSGQILFERWCIGLYNVMFTAMPPLTLGIFERSCRKENMLKYPELYKTSQNALDFNTKVFWVHCLNGLFHSVILFWFPLKALQYGTVFGNGKTSDYLLLGNFVYTFVVITVCLKAGLETSYWTWFSHIAIWGSIALWVVFFGIYSSLWPAVPMAPDMSGEAAMLFSSGVFWVGLLSIPVASLLLDVLYKVIKRTAFKTLVDEVQELEAKSQDPGAVVLGKSLTERAQLLKNVFKKNHVNLYRSESLQQNLLHGYAFSQDENGIVSQSEVIRAYDTTKQRPDEW

Gene Information

Entrez Gene ID
11980
Gene Name
ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Gene Symbol
Atp8a1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031410 IEA:UniProtKB-KW C cytoplasmic vesicle
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0019829 IEA:InterPro F cation-transporting ATPase activity
GO:0000287 IEA:InterPro F magnesium ion binding
GO:0004012 IEA:UniProtKB-EC F phospholipid-translocating ATPase activity
GO:0007612 IMP:UniProtKB P learning
GO:0030335 IMP:UniProtKB P positive regulation of cell migration
GO:0061092 IDA:UniProtKB P positive regulation of phospholipid translocation

REACTOME Pathway Links

REACTOME Pathway ID Description
5893745 Ion channel transport
5893744 Ion transport by P-type ATPases
5892775 Transmembrane transport of small molecules

Domain Information

InterPro Annotations

Accession Description
IPR008250 ATPase_P-typ_transduc_dom_A
IPR001757 Cation_transp_P_typ_ATPase
IPR023214 HAD-like domain
IPR023299 P-type ATPase, cytoplasmic domain N
IPR018303 P-type ATPase, phosphorylation site
IPR006539 P-type ATPase, subfamily IV

UniProt Annotations

Entry Information

Gene Name
ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Protein Entry
P70704_MOUSE
UniProt ID
P70704
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P70704-1; Sequence=Displayed; Name=2; IsoId=P70704-2; Sequence=VSP_055306, VSP_055307; Name=3; IsoId=P70704-3; Sequence=VSP_055307;
Catalytic Activity ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).
Caution Initial characterization studies with purified Atp8a1 enzyme demonstrated similar but distinct properties compared to the plasma membrane aminophospholipid flippase; however, the flippase complex accessory beta subunit was not included in the assays. {ECO:0000305|PubMed:16618126}.
Enzyme Regulation ATPase activity is stimulated by phosphatidylserine (PS) and minimally by phosphatidylethanolamine (PE). {ECO:0000269|PubMed:16618126}.
Function Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Acts as aminophospholipid translocase at the plasma membrane in neuronal cells; the activity is associated with hippocampus-dependent learning. {ECO:0000269|PubMed:16618126, ECO:0000269|PubMed:20224745, ECO:0000269|PubMed:22007859, ECO:0000269|PubMed:23269685}.
Similarity Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. {ECO:0000305}.
Subcellular Location Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Cytoplasmic granule {ECO:0000250}. Cell membrane {ECO:0000250}. Endoplasmic reticulum {ECO:0000250}. Golgi apparatus {ECO:0000250}. Note=Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B. In the presence of TMEM30A, predominantly located in cytoplasmic punctate structures (By similarity). Localizes to plasma membranes of red blood cells. {ECO:0000250, ECO:0000269|PubMed:16643453}.
Subunit Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit. Interacts with TMEM30A to form a flippase complex. {ECO:0000269|PubMed:23269685}.
Tissue Specificity Found in most tissues except liver and testis. Most abundant in brain and lung. Also detected in fetal tissues. Isoform 1 is expressed in brain. Isoform 2 and isoform 3 are expressed in reticulocytes. {ECO:0000269|PubMed:16643453}.

Identical and Related Proteins

Unique RefSeq proteins for LMP001611 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
84781802 RefSeq NP_001034088 1164 phospholipid-transporting ATPase IA isoform a
7106257 RefSeq NP_033857 1149 phospholipid-transporting ATPase IA isoform b
547234934 RefSeq NP_001271274 1164 phospholipid-transporting ATPase IA isoform c

Identical Sequences to LMP001611 proteins

Reference Database Accession Length Protein Name
GI:84781802 DBBJ BAC32330.1 1164 unnamed protein product [Mus musculus]
GI:84781802 DBBJ BAE24734.1 1164 unnamed protein product [Mus musculus]
GI:7106257 GenBank AAB18627.1 1149 chromaffin granule ATPase II homolog [Mus musculus]
GI:7106257 GenBank EDL37788.1 1149 ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1, isoform CRA_a [Mus musculus]
GI:84781802 SwissProt P70704.2 1164 RecName: Full=Phospholipid-transporting ATPase IA; AltName: Full=ATPase class I type 8A member 1; AltName: Full=Chromaffin granule ATPase II; AltName: Full=P4-ATPase flippase complex alpha subunit ATP8A1 [Mus musculus]

Related Sequences to LMP001611 proteins

Reference Database Accession Length Protein Name
GI:84781802 DBBJ BAD90541.1 1195 mKIAA4233 protein, partial [Mus musculus]
GI:547234934 DBBJ BAE24734.1 1164 unnamed protein product [Mus musculus]
GI:7106257 GenBank AAI29873.1 1161 Atp8a1 protein [Mus musculus]
GI:547234934 GenBank AAI29873.1 1161 Atp8a1 protein [Mus musculus]
GI:84781802 GenBank EDL37789.1 1195 ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1, isoform CRA_b, partial [Mus musculus]
GI:547234934 RefSeq NP_001034088.1 1164 phospholipid-transporting ATPase IA isoform a [Mus musculus]
GI:84781802 RefSeq XP_005074411.1 1164 PREDICTED: probable phospholipid-transporting ATPase IA isoform X1 [Mesocricetus auratus]
GI:7106257 RefSeq XP_005074412.1 1149 PREDICTED: probable phospholipid-transporting ATPase IA isoform X2 [Mesocricetus auratus]
GI:84781802 RefSeq XP_005359354.1 1164 PREDICTED: probable phospholipid-transporting ATPase IA isoform X1 [Microtus ochrogaster]
GI:547234934 RefSeq XP_005359355.1 1164 PREDICTED: probable phospholipid-transporting ATPase IA isoform X2 [Microtus ochrogaster]
GI:7106257 RefSeq XP_005359356.1 1149 PREDICTED: probable phospholipid-transporting ATPase IA isoform X3 [Microtus ochrogaster]
GI:7106257 RefSeq NP_001271274.1 1164 phospholipid-transporting ATPase IA isoform c [Mus musculus]
GI:84781802 RefSeq NP_001271274.1 1164 phospholipid-transporting ATPase IA isoform c [Mus musculus]
GI:84781802 RefSeq XP_006974696.1 1164 PREDICTED: probable phospholipid-transporting ATPase IA isoform X1 [Peromyscus maniculatus bairdii]
GI:547234934 RefSeq XP_006974697.1 1164 PREDICTED: probable phospholipid-transporting ATPase IA isoform X2 [Peromyscus maniculatus bairdii]
GI:7106257 RefSeq XP_006974697.1 1164 PREDICTED: probable phospholipid-transporting ATPase IA isoform X2 [Peromyscus maniculatus bairdii]
GI:7106257 RefSeq XP_006974698.1 1149 PREDICTED: probable phospholipid-transporting ATPase IA isoform X3 [Peromyscus maniculatus bairdii]
GI:547234934 SwissProt P70704.2 1164 RecName: Full=Phospholipid-transporting ATPase IA; AltName: Full=ATPase class I type 8A member 1; AltName: Full=Chromaffin granule ATPase II; AltName: Full=P4-ATPase flippase complex alpha subunit ATP8A1 [Mus musculus]