Gene/Proteome Database (LMPD)
LMPD ID
LMP001706
Gene ID
Species
Homo sapiens (Human)
Gene Name
choline kinase beta
Gene Symbol
Synonyms
CHETK; CHKL; CK; CKB; CKEKB; EK; EKB; MDCMC
Alternate Names
choline/ethanolamine kinase; ethanolamine kinase beta; choline kinase-like protein
Chromosome
22
Map Location
22q13.33
EC Number
2.7.1.32
Summary
Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]
Orthologs
Proteins
| choline/ethanolamine kinase | |
|---|---|
| Refseq ID | NP_005189 |
| Protein GI | 6978649 |
| UniProt ID | Q9Y259 |
| mRNA ID | NM_005198 |
| Length | 395 |
| RefSeq Status | REVIEWED |
| MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSLKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVHSSS | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | TAS:Reactome | C | cytosol |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0004103 | IDA:UniProtKB | F | choline kinase activity |
| GO:0004305 | IDA:UniProtKB | F | ethanolamine kinase activity |
| GO:0006657 | IDA:GOC | P | CDP-choline pathway |
| GO:0046474 | TAS:Reactome | P | glycerophospholipid biosynthetic process |
| GO:0007517 | IEA:Ensembl | P | muscle organ development |
| GO:0006656 | TAS:Reactome | P | phosphatidylcholine biosynthetic process |
| GO:0006646 | IDA:UniProtKB | P | phosphatidylethanolamine biosynthetic process |
| GO:0006644 | TAS:Reactome | P | phospholipid metabolic process |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_121238 | Synthesis of PC |
| REACT_120919 | Synthesis of PE |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR011009 | Protein kinase-like domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9Y259-1; Sequence=Displayed; Name=2; IsoId=Q9Y259-2; Sequence=VSP_034248, VSP_034249; |
| Biophysicochemical Properties | Kinetic parameters: KM=0.57 mM for choline ; KM=2.9 mM for ethanolamine ; |
| Catalytic Activity | ATP + choline = ADP + phosphocholine. |
| Catalytic Activity | ATP + ethanolamine = ADP + O- phosphoethanolamine. |
| Function | Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis. |
| Miscellaneous | This protein is produced by a bicistronic gene which also produces the CPT1B protein from a non-overlapping reading frame. |
| Pathway | Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3. |
| Sequence Caution | Sequence=AAB03342.2; Type=Erroneous gene model prediction; Evidence= ; |
| Similarity | Belongs to the choline/ethanolamine kinase family. |
| Subunit | Homodimer, and heterodimer with CHKA. |
Identical and Related Proteins
Unique RefSeq proteins for LMP001706 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 6978649 | RefSeq | NP_005189 | 395 | choline/ethanolamine kinase |
Identical Sequences to LMP001706 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6978649 | DBBJ | BAG36972.1 | 395 | unnamed protein product [Homo sapiens] |
| GI:6978649 | DBBJ | BAI46268.1 | 395 | choline kinase beta, partial [synthetic construct] |
| GI:6978649 | GenBank | AAI01489.1 | 395 | Choline kinase beta [Homo sapiens] |
| GI:6978649 | GenBank | ACP56755.1 | 395 | Sequence 884 from patent US 7482117 |
| GI:6978649 | GenBank | ADR82980.1 | 395 | choline kinase beta, partial [synthetic construct] |
| GI:6978649 | GenBank | ADS30352.1 | 395 | Sequence 884 from patent US 7781168 |
Related Sequences to LMP001706 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6978649 | GenBank | AAQ02522.1 | 396 | choline kinase-like, partial [synthetic construct] |
| GI:6978649 | GenBank | JAA00506.1 | 395 | choline kinase beta [Pan troglodytes] |
| GI:6978649 | GenBank | JAA11355.1 | 395 | choline kinase beta [Pan troglodytes] |
| GI:6978649 | GenBank | JAA27973.1 | 395 | choline kinase beta [Pan troglodytes] |
| GI:6978649 | RefSeq | XP_001144556.1 | 395 | PREDICTED: choline/ethanolamine kinase isoform X1 [Pan troglodytes] |
| GI:6978649 | RefSeq | XP_003804638.1 | 395 | PREDICTED: choline/ethanolamine kinase isoform X1 [Pan paniscus] |