Gene/Proteome Database (LMPD)
LMPD ID
LMP001764
Gene ID
Species
Homo sapiens (Human)
Gene Name
1-acylglycerol-3-phosphate O-acyltransferase 5
Gene Symbol
Synonyms
1AGPAT5; LPAATE
Alternate Names
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; 1-AGPAT 5; 1-AGP acyltransferase 5; lysophosphatidic acid acyltransferase epsilon; 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon)
Chromosome
8
Map Location
8p23.1
EC Number
2.3.1.51
Summary
This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. A pseudogene of this gene is present on the Y chromosome. [provided by RefSeq, Aug 2014]
Orthologs
Proteins
| 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon | |
|---|---|
| Refseq ID | NP_060831 |
| Protein GI | 61743952 |
| UniProt ID | Q9NUQ2 |
| mRNA ID | NM_018361 |
| Length | 364 |
| RefSeq Status | REVIEWED |
| MLLSLVLHTYSMRYLLPSVVLLGTAPTYVLAWGVWRLLSAFLPARFYQALDDRLYCVYQSMVLFFFENYTGVQILLYGDLPKNKENIIYLANHQSTVDWIVADILAIRQNALGHVRYVLKEGLKWLPLYGCYFAQHGGIYVKRSAKFNEKEMRNKLQSYVDAGTPMYLVIFPEGTRYNPEQTKVLSASQAFAAQRGLAVLKHVLTPRIKATHVAFDCMKNYLDAIYDVTVVYEGKDDGGQRRESPTMTEFLCKECPKIHIHIDRIDKKDVPEEQEHMRRWLHERFEIKDKMLIEFYESPDPERRKRFPGKSVNSKLSIKKTLPSMLILSGLTAGMLMTDAGRKLYVNTWIYGTLLGCLWVTIKA | |
Gene Information
Entrez Gene ID
Gene Name
1-acylglycerol-3-phosphate O-acyltransferase 5
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005789 | TAS:Reactome | C | endoplasmic reticulum membrane |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0005741 | TAS:Reactome | C | mitochondrial outer membrane |
| GO:0005739 | IDA:LIFEdb | C | mitochondrion |
| GO:0005634 | IEA:UniProtKB-KW | C | nucleus |
| GO:0003841 | NAS:UniProtKB | F | 1-acylglycerol-3-phosphate O-acyltransferase activity |
| GO:0016024 | IEA:UniProtKB-UniPathway | P | CDP-diacylglycerol biosynthetic process |
| GO:0044255 | TAS:Reactome | P | cellular lipid metabolic process |
| GO:0046474 | TAS:Reactome | P | glycerophospholipid biosynthetic process |
| GO:0002244 | IEA:Ensembl | P | hematopoietic progenitor cell differentiation |
| GO:0006654 | TAS:Reactome | P | phosphatidic acid biosynthetic process |
| GO:0008654 | NAS:UniProtKB | P | phospholipid biosynthetic process |
| GO:0006644 | TAS:Reactome | P | phospholipid metabolic process |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0019432 | TAS:Reactome | P | triglyceride biosynthetic process |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| PWY-5667 | CDP-diacylglycerol biosynthesis I |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_121401 | Glycerophospholipid biosynthesis |
| REACT_120906 | Synthesis of PA |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR002123 | Phospholipid/glycerol acyltransferase |
UniProt Annotations
Entry Information
Gene Name
1-acylglycerol-3-phosphate O-acyltransferase 5
Protein Entry
PLCE_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=5.52 uM for LPA sn-1 C18:1; KM=16.30 uM for C18:1-CoA; Vmax=2.42 nmol/min/mg enzyme toward LPA sn-1 C18:1; Vmax=1.22 nmol/min/mg enzyme toward C18:1-CoA; |
| Catalytic Activity | Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. |
| Caution | It is uncertain whether Met-1 or Met-12 is the initiator. |
| Domain | The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. |
| Function | Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor. Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA. Activity toward lysophosphatidylglycerol not detectable. |
| Pathway | Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3. |
| Sequence Caution | Sequence=BAA92069.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence= ; |
| Similarity | Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. |
| Subcellular Location | Endoplasmic reticulum membrane ; Multi-pass membrane protein . Nucleus envelope . Mitochondrion . |
| Tissue Specificity | Widely expressed. |
Identical and Related Proteins
Unique RefSeq proteins for LMP001764 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 61743952 | RefSeq | NP_060831 | 364 | 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon |
Identical Sequences to LMP001764 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:61743952 | DBBJ | BAI46224.1 | 364 | 1-acylglycerol-3-phosphate O-acyltransferase 5, partial [synthetic construct] |
| GI:61743952 | GenBank | ABP22945.1 | 364 | Sequence 5 from patent US 7189833 |
| GI:61743952 | GenBank | ABY09473.1 | 364 | Sequence 63 from patent US 7301016 |
| GI:61743952 | GenBank | ADS47653.1 | 364 | Sequence 25 from patent US 7799898 |
| GI:61743952 | GenBank | AFQ88726.1 | 364 | Sequence 25 from patent US 8252915 |
| GI:61743952 | GenBank | AHD78792.1 | 364 | Sequence 28436 from patent US 8586006 |
Related Sequences to LMP001764 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:61743952 | GenBank | JAA07705.1 | 364 | 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) [Pan troglodytes] |
| GI:61743952 | GenBank | JAA11719.1 | 364 | 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) [Pan troglodytes] |
| GI:61743952 | GenBank | JAA23440.1 | 364 | 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) [Pan troglodytes] |
| GI:61743952 | GenBank | JAA34976.1 | 364 | 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) [Pan troglodytes] |
| GI:61743952 | RefSeq | XP_519588.1 | 364 | PREDICTED: 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon [Pan troglodytes] |
| GI:61743952 | RefSeq | NP_001124637.1 | 364 | 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon [Pongo abelii] |