Gene/Proteome Database (LMPD)
LMPD ID
LMP001796
Gene ID
Species
Mus musculus (Mouse)
Gene Name
pyruvate dehydrogenase kinase, isoenzyme 2
Gene Symbol
Synonyms
-
Alternate Names
pyruvate dehydrogenase kinase, isoenzyme 2; PDH kinase 2; pyruvate dehydrogenase 2; pyruvate dehydrogenase kinase, isozyme 2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
Chromosome
11
Map Location
11 D|11 59.01 cM
EC Number
2.7.11.2
Proteins
| pyruvate dehydrogenase kinase, isoenzyme 2 | |
|---|---|
| Refseq ID | NP_598428 |
| Protein GI | 226958643 |
| UniProt ID | Q9JK42 |
| mRNA ID | NM_133667 |
| Length | 407 |
| RefSeq Status | VALIDATED |
| MRWVRALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVLGTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCSVSDVVKDAYDMAKLLCDKYYMASPDLEIQEVNATNANQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLTLPPIKIMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS | |
Gene Information
Entrez Gene ID
Gene Name
pyruvate dehydrogenase kinase, isoenzyme 2
Gene Symbol
Species
Mus musculus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005967 | IDA:UniProtKB | C | mitochondrial pyruvate dehydrogenase complex |
| GO:0005739 | IDA:UniProtKB | C | mitochondrion |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0004674 | IEA:UniProtKB-KW | F | protein serine/threonine kinase activity |
| GO:0004740 | IMP:UniProtKB | F | pyruvate dehydrogenase (acetyl-transferring) kinase activity |
| GO:0031670 | IMP:UniProtKB | P | cellular response to nutrient |
| GO:0034614 | IEA:Ensembl | P | cellular response to reactive oxygen species |
| GO:0042593 | IMP:UniProtKB | P | glucose homeostasis |
| GO:0006006 | IEA:UniProtKB-KW | P | glucose metabolic process |
| GO:0008286 | IMP:UniProtKB | P | insulin receptor signaling pathway |
| GO:0072332 | IEA:Ensembl | P | intrinsic apoptotic signaling pathway by p53 class mediator |
| GO:0006468 | IMP:GOC | P | protein phosphorylation |
| GO:0010510 | IMP:UniProtKB | P | regulation of acetyl-CoA biosynthetic process from pyruvate |
| GO:0010565 | IMP:UniProtKB | P | regulation of cellular ketone metabolic process |
| GO:0006111 | IMP:UniProtKB | P | regulation of gluconeogenesis |
| GO:0010906 | IMP:UniProtKB | P | regulation of glucose metabolic process |
| GO:0006885 | IMP:UniProtKB | P | regulation of pH |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Gene Name
pyruvate dehydrogenase kinase, isoenzyme 2
Protein Entry
PDK2_MOUSE
UniProt ID
Species
Mouse
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + [pyruvate dehydrogenase (acetyl- transferring)] = ADP + [pyruvate dehydrogenase (acetyl- transferring)] phosphate. |
| Disruption Phenotype | No visible phenotype, due to the fact that PDK2-deficient mice have increased PDK1 levels (PubMed:21411764). Mice have lower blood glucose levels in the fed state, but not after fasting. Likewise, they display increased pyruvate dehydrogenase activity in liver and skeletal muscle in the fed state, but not after fasting. Fasting mice lacking both PDK2 and PDK4 show strongly decreased blood glucose levels, increased circulating ketone body levels leading to ketoacidosis with dangerously low blood pH levels, hypothermia, and ultimately death (PubMed:22360721). {ECO:0000269|PubMed:21411764, ECO:0000269|PubMed:22360721}. |
| Function | Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis. {ECO:0000269|PubMed:22360721}. |
| Induction | Up-regulated by glucose and palmitic acid. Up-regulated by PPARD. {ECO:0000269|PubMed:16631612, ECO:0000269|PubMed:17669420}. |
| Interaction | O08908:Pik3r2; NbExp=3; IntAct=EBI-643530, EBI-643570; |
| Similarity | Belongs to the PDK/BCKDK protein kinase family. {ECO:0000305}. |
| Similarity | Contains 1 histidine kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00107}. |
| Subcellular Location | Mitochondrion matrix {ECO:0000269|PubMed:21190881}. |
| Subunit | Homodimer, and heterodimer with PDK1. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). {ECO:0000269|PubMed:21190881}. |
| Tissue Specificity | Detected in heart (at protein level). {ECO:0000269|PubMed:21190881}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP001796 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 226958643 | RefSeq | NP_598428 | 407 | pyruvate dehydrogenase kinase, isoenzyme 2 |
Identical Sequences to LMP001796 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:226958643 | GenBank | AAH21764.1 | 407 | Pyruvate dehydrogenase kinase, isoenzyme 2 [Mus musculus] |
| GI:226958643 | GenBank | ABK42391.1 | 407 | PDHK2 [synthetic construct] |
| GI:226958643 | GenBank | EDL15963.1 | 407 | pyruvate dehydrogenase kinase, isoenzyme 2, isoform CRA_a [Mus musculus] |
| GI:226958643 | RefSeq | XP_006971999.1 | 407 | PREDICTED: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial isoform X1 [Peromyscus maniculatus bairdii] |
| GI:226958643 | SwissProt | Q9JK42.2 | 407 | RecName: Full=[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial; AltName: Full=Pyruvate dehydrogenase kinase isoform 2; Short=PDH kinase 2; Flags: Precursor [Mus musculus] |
Related Sequences to LMP001796 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:226958643 | GenBank | AAF72038.1 | 407 | pyruvate dehydrogenase kinase 2 [Mus musculus] |
| GI:226958643 | GenBank | EGW01786.1 | 407 | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial [Cricetulus griseus] |
| GI:226958643 | RefSeq | NP_110499.1 | 407 | pyruvate dehydrogenase kinase, isozyme 2 [Rattus norvegicus] |
| GI:226958643 | RefSeq | XP_003504004.1 | 407 | PREDICTED: pyruvate dehydrogenase kinase, isozyme 2 isoform X1 [Cricetulus griseus] |
| GI:226958643 | RefSeq | XP_005075913.1 | 407 | PREDICTED: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial isoform X1 [Mesocricetus auratus] |
| GI:226958643 | RefSeq | XP_008836552.1 | 407 | PREDICTED: pyruvate dehydrogenase kinase, isozyme 2 [Nannospalax galili] |