Gene/Proteome Database (LMPD)

LMPD ID
LMP001934
Gene ID
19224
Species
Mus musculus (Mouse)
Gene Name
prostaglandin-endoperoxide synthase 1
Gene Symbol
Ptgs1
Synonyms
COX1; Cox-1; Cox-3; PGHS-1; PHS 1; Pghs1
Alternate Names
prostaglandin G/H synthase 1; PGH synthase 1; cyclooxygenase-1; prostaglandin H2 synthase 1
Chromosome
2
Map Location
2 B|2 24.19 cM
EC Number
1.14.99.1
Summary
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. [provided by RefSeq, Jan 2014]
Orthologs
View orthologs and multiple alignments for Ptgs1

Proteins

prostaglandin G/H synthase 1 precursor
Refseq ID NP_032995
Protein GI 6679537
UniProt ID P22437
mRNA ID NM_008969
Length 602
RefSeq Status REVIEWED
MSRRSLSLWFPLLLLLLLPPTPSVLLADPGVPSPVNPCCYYPCQNQGVCVRFGLDNYQCDCTRTGYSGPNCTIPEIWTWLRNSLRPSPSFTHFLLTHGYWLWEFVNATFIREVLMRLVLTVRSNLIPSPPTYNSAHDYISWESFSNVSYYTRILPSVPKDCPTPMGTKGKKQLPDVQLLAQQLLLRREFIPAPQGTNILFAFFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYHLRLFKDGKLKYQVLDGEVYPPSVEQASVLMRYPPGVPPERQMAVGQEVFGLLPGLMLFSTIWLREHNRVCDLLKEEHPTWDDEQLFQTTRLILIGETIKIVIEEYVQHLSGYFLQLKFDPELLFRAQFQYRNRIAMEFNHLYHWHPLMPNSFQVGSQEYSYEQFLFNTSMLVDYGVEALVDAFSRQRAGRIGGGRNFDYHVLHVAVDVIKESREMRLQPFNEYRKRFGLKPYTSFQELTGEKEMAAELEELYGDIDALEFYPGLLLEKCQPNSIFGESMIEMGAPFSLKGLLGNPICSPEYWKPSTFGGDVGFNLVNTASLKKLVCLNTKTCPYVSFRVPDYPGDDGSVLVRRSTEL
sig_peptide: 1..26 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P22437.1) calculated_mol_wt: 2977 peptide sequence: MSRRSLSLWFPLLLLLLLPPTPSVLL mat_peptide: 27..602 product: Prostaglandin G/H synthase 1 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P22437.1) calculated_mol_wt: 66084 peptide sequence: ADPGVPSPVNPCCYYPCQNQGVCVRFGLDNYQCDCTRTGYSGPNCTIPEIWTWLRNSLRPSPSFTHFLLTHGYWLWEFVNATFIREVLMRLVLTVRSNLIPSPPTYNSAHDYISWESFSNVSYYTRILPSVPKDCPTPMGTKGKKQLPDVQLLAQQLLLRREFIPAPQGTNILFAFFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYHLRLFKDGKLKYQVLDGEVYPPSVEQASVLMRYPPGVPPERQMAVGQEVFGLLPGLMLFSTIWLREHNRVCDLLKEEHPTWDDEQLFQTTRLILIGETIKIVIEEYVQHLSGYFLQLKFDPELLFRAQFQYRNRIAMEFNHLYHWHPLMPNSFQVGSQEYSYEQFLFNTSMLVDYGVEALVDAFSRQRAGRIGGGRNFDYHVLHVAVDVIKESREMRLQPFNEYRKRFGLKPYTSFQELTGEKEMAAELEELYGDIDALEFYPGLLLEKCQPNSIFGESMIEMGAPFSLKGLLGNPICSPEYWKPSTFGGDVGFNLVNTASLKKLVCLNTKTCPYVSFRVPDYPGDDGSVLVRRSTEL

Gene Information

Entrez Gene ID
19224
Gene Name
prostaglandin-endoperoxide synthase 1
Gene Symbol
Ptgs1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:MGI C cytoplasm
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0001750 IDA:MGI C photoreceptor outer segment
GO:0051213 IEA:UniProtKB-KW F dioxygenase activity
GO:0020037 IEA:InterPro F heme binding
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0004601 IEA:UniProtKB-KW F peroxidase activity
GO:0004666 IEA:UniProtKB-EC F prostaglandin-endoperoxide synthase activity
GO:0019371 IEA:Ensembl P cyclooxygenase pathway
GO:0006954 IEA:InterPro P inflammatory response
GO:0030216 NAS:UniProtKB P keratinocyte differentiation
GO:0001516 IMP:MGI P prostaglandin biosynthetic process
GO:0006693 IMP:MGI P prostaglandin metabolic process
GO:0008217 IMP:MGI P regulation of blood pressure
GO:0042127 IGI:MGI P regulation of cell proliferation
GO:0006979 IEA:InterPro P response to oxidative stress

KEGG Pathway Links

KEGG Pathway ID Description
mmu04611 Platelet activation
mmu04726 Serotonergic synapse

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY3DJ-35583 biosynthesis of prostaglandins

Domain Information

InterPro Annotations

Accession Description
IPR000742 Epidermal growth factor-like domain
IPR019791 Haem peroxidase, animal
IPR010255 Haem_peroxidase
IPR029580 Prostaglandin G/H synthase 1

UniProt Annotations

Entry Information

Gene Name
prostaglandin-endoperoxide synthase 1
Protein Entry
PGH1_MOUSE
UniProt ID
P22437
Species
Mouse

Comments

Comment Type Description
Catalytic Activity Arachidonate + AH(2) + 2 O(2) = prostaglandin H(2) + A + H(2)O.
Cofactor Name=heme b; Xref=ChEBI:CHEBI:60344; Evidence={ECO:0000250}; Note=Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit. {ECO:0000250};
Function Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
Miscellaneous Conversion of arachidonate to prostaglandin H2 is mediated by 2 different isozymes: the constitutive PTGS1 and the inducible PTGS2. PGHS1 is expressed constitutively and generally produces prostanoids acutely in response to hormonal stimuli to fine-tune physiological processes requiring instantaneous, continuous regulation (e.g. hemostasis). PGHS2 is inducible and typically produces prostanoids that mediate responses to physiological stresses such as infection and inflammation.
Miscellaneous PTGS1 and PTGS2 are the targets of nonsteroidal anti-inflammatory drugs (NSAIDs) including aspirin and ibuprofen. Aspirin is able to produce an irreversible inactivation of the enzyme through a serine acetylation. Inhibition of the PGHSs with NSAIDs acutely reduces inflammation, pain, and fever, and long- term use of these drugs reduces fatal thrombotic events, as well as the development of colon cancer and Alzheimer's disease. PTGS2 is the principal isozyme responsible for production of inflammatory prostaglandins. New generation PTGSs inhibitors strive to be selective for PTGS2, to avoid side effects such as gastrointestinal complications and ulceration.
Miscellaneous The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). The cyclooxygenase reaction occurs in a hydrophobic channel in the core of the enzyme. The peroxidase reaction occurs at a heme- containing active site located near the protein surface. The nonsteroidal anti-inflammatory drugs (NSAIDs) binding site corresponds to the cyclooxygenase active site.
Pathway Lipid metabolism; prostaglandin biosynthesis.
Similarity Belongs to the prostaglandin G/H synthase family. {ECO:0000305}.
Similarity Contains 1 EGF-like domain. {ECO:0000255|PROSITE- ProRule:PRU00076}.
Subcellular Location Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
Subunit Homodimer.

Identical and Related Proteins

Unique RefSeq proteins for LMP001934 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6679537 RefSeq NP_032995 602 prostaglandin G/H synthase 1 precursor

Identical Sequences to LMP001934 proteins

Reference Database Accession Length Protein Name
GI:6679537 GenBank ACW56523.1 602 Sequence 7 from patent US 7588758
GI:6679537 GenBank ADA08500.1 602 Sequence 51 from patent US 7601816
GI:6679537 RefSeq XP_006497853.1 602 PREDICTED: prostaglandin G/H synthase 1 isoform X1 [Mus musculus]
GI:6679537 RefSeq XP_006497854.1 602 PREDICTED: prostaglandin G/H synthase 1 isoform X2 [Mus musculus]
GI:6679537 RefSeq XP_006497855.1 602 PREDICTED: prostaglandin G/H synthase 1 isoform X3 [Mus musculus]
GI:6679537 RefSeq XP_006497856.1 602 PREDICTED: prostaglandin G/H synthase 1 isoform X4 [Mus musculus]

Related Sequences to LMP001934 proteins

Reference Database Accession Length Protein Name
GI:6679537 DBBJ BAE22038.1 602 unnamed protein product [Mus musculus]
GI:6679537 GenBank AAH81816.1 602 Ptgs1 protein [Rattus norvegicus]
GI:6679537 GenBank ABP06351.1 602 Sequence 52 from patent US 7179627
GI:6679537 GenBank EDL93122.1 602 rCG45926, isoform CRA_a [Rattus norvegicus]
GI:6679537 RefSeq NP_058739.4 602 prostaglandin G/H synthase 1 precursor [Rattus norvegicus]
GI:6679537 SwissProt Q63921.2 602 RecName: Full=Prostaglandin G/H synthase 1; AltName: Full=Cyclooxygenase-1; Short=COX-1; AltName: Full=Prostaglandin H2 synthase 1; Short=PGH synthase 1; Short=PGHS-1; Short=PHS 1; AltName: Full=Prostaglandin-endoperoxide synthase 1; Flags: Precursor [Rattus norvegicus]