Gene/Proteome Database (LMPD)
LMPD ID
LMP001959
Gene ID
Species
Homo sapiens (Human)
Gene Name
sphingosine kinase 2
Gene Symbol
Synonyms
SK 2; SK-2; SPK 2; SPK-2
Chromosome
19
Map Location
19q13.2
EC Number
2.7.1.91
Summary
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Orthologs
Proteins
| sphingosine kinase 2 isoform a | |
|---|---|
| Refseq ID | NP_064511 |
| Protein GI | 21361699 |
| UniProt ID | Q9NRA0 |
| mRNA ID | NM_020126 |
| Length | 654 |
| RefSeq Status | REVIEWED |
| MNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAKAMAPPPPPLAASTPLLHGEFGSYPARGPRFALTLTSQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRSRSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGAATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP | |
| sphingosine kinase 2 isoform a | |
|---|---|
| Refseq ID | NP_001191088 |
| Protein GI | 323462187 |
| UniProt ID | Q9NRA0 |
| mRNA ID | NM_001204159 |
| Length | 654 |
| RefSeq Status | REVIEWED |
| Protein sequence is identical to GI:21361699 (mRNA isoform) | |
| sphingosine kinase 2 isoform b | |
|---|---|
| Refseq ID | NP_001191087 |
| Protein GI | 323462183 |
| UniProt ID | Q9NRA0 |
| mRNA ID | NM_001204158 |
| Length | 595 |
| RefSeq Status | REVIEWED |
| MNGHLEAEEQQDQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRSRSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGAATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP | |
| sphingosine kinase 2 isoform c | |
|---|---|
| Refseq ID | NP_001191089 |
| Protein GI | 323462185 |
| UniProt ID | Q9NRA0 |
| mRNA ID | NM_001204160 |
| Length | 618 |
| RefSeq Status | REVIEWED |
| MAPPPPPLAASTPLLHGEFGSYPARGPRFALTLTSQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRSRSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGAATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP | |
| sphingosine kinase 2 isoform d | |
|---|---|
| Refseq ID | NP_001230805 |
| Protein GI | 345197213 |
| UniProt ID | E9PCV4 |
| mRNA ID | NM_001243876 |
| Length | 448 |
| RefSeq Status | REVIEWED |
| MISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | IDA:UniProtKB | C | cytosol |
| GO:0005765 | IDA:UniProtKB | C | lysosomal membrane |
| GO:0016020 | IDA:UniProtKB | C | membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0017050 | IEA:Ensembl | F | D-erythro-sphingosine kinase activity |
| GO:0003951 | IEA:InterPro | F | NAD+ kinase activity |
| GO:0017016 | NAS:UniProtKB | F | Ras GTPase binding |
| GO:0004143 | IEA:InterPro | F | diacylglycerol kinase activity |
| GO:0008481 | NAS:UniProtKB | F | sphinganine kinase activity |
| GO:0001568 | IEA:Ensembl | P | blood vessel development |
| GO:0007420 | IEA:Ensembl | P | brain development |
| GO:0008283 | NAS:UniProtKB | P | cell proliferation |
| GO:0046834 | NAS:GOC | P | lipid phosphorylation |
| GO:0043066 | NAS:UniProtKB | P | negative regulation of apoptotic process |
| GO:0008284 | IEA:Ensembl | P | positive regulation of cell proliferation |
| GO:0007205 | IEA:InterPro | P | protein kinase C-activating G-protein coupled receptor signaling pathway |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0006669 | NAS:UniProtKB | P | sphinganine-1-phosphate biosynthetic process |
| GO:0030148 | TAS:Reactome | P | sphingolipid biosynthetic process |
| GO:0006665 | TAS:Reactome | P | sphingolipid metabolic process |
| GO:0006670 | IEA:Ensembl | P | sphingosine metabolic process |
KEGG Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=5; Comment=Experimental confirmation may be lacking for some isoforms.; Name=1; IsoId=Q9NRA0-1; Sequence=Displayed; Name=2; IsoId=Q9NRA0-2; Sequence=VSP_006217; Name=3; IsoId=Q9NRA0-3; Sequence=VSP_006217, VSP_006218; Name=4; IsoId=Q9NRA0-4; Sequence=VSP_046910; Note=No experimental confirmation available.; Name=5; IsoId=Q9NRA0-5; Sequence=VSP_047721, VSP_047722; |
| Biophysicochemical Properties | Kinetic parameters: KM=3.4 uM for sphingosine ; pH dependence: Optimum pH is 7.5. ; |
| Catalytic Activity | ATP + sphinganine = ADP + sphinganine 1- phosphate. |
| Catalytic Activity | ATP + sphingosine = ADP + sphingosine 1- phosphate. |
| Cofactor | Name=Mg(2+); Xref=ChEBI |
| Enzyme Regulation | Inhibited by sulfatide. |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. |
| Similarity | Contains 1 DAGKc domain. {ECO |
| Subcellular Location | Isoform 1: Cytoplasm. Membrane. |
| Subcellular Location | Isoform 2: Lysosome membrane. |
Identical and Related Proteins
Unique RefSeq proteins for LMP001959 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 21361699 | RefSeq | NP_064511 | 654 | sphingosine kinase 2 isoform a |
| 323462183 | RefSeq | NP_001191087 | 595 | sphingosine kinase 2 isoform b |
| 323462185 | RefSeq | NP_001191089 | 618 | sphingosine kinase 2 isoform c |
| 345197213 | RefSeq | NP_001230805 | 448 | sphingosine kinase 2 isoform d |
Identical Sequences to LMP001959 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:323462183 | DBBJ | BAG62249.1 | 595 | unnamed protein product [Homo sapiens] |
| GI:323462185 | EMBL | CBH26133.1 | 618 | unnamed protein product [Homo sapiens] |
| GI:323462185 | EMBL | CBX51413.1 | 618 | unnamed protein product [Homo sapiens] |
| GI:345197213 | GenBank | EAW52371.1 | 448 | sphingosine kinase 2, isoform CRA_e [Homo sapiens] |
| GI:21361699 | GenBank | ABW03921.1 | 654 | sphingosine kinase 2 [synthetic construct] |
| GI:323462185 | GenBank | ACJ97776.1 | 618 | Sequence 14 from patent US 7419814 |
| GI:323462185 | GenBank | ADS49014.1 | 618 | Sequence 14 from patent US 7803595 |
| GI:21361699 | GenBank | AED82287.1 | 654 | Sequence 1024 from patent US 7919467 |
| GI:323462185 | GenBank | AFS96148.1 | 618 | Sequence 14 from patent US 8263349 |
| GI:21361699 | GenBank | AHD75998.1 | 654 | Sequence 18381 from patent US 8586006 |
| GI:21361699 | GenBank | AIC62777.1 | 654 | SPHK2, partial [synthetic construct] |
| GI:21361699 | RefSeq | NP_001191088.1 | 654 | sphingosine kinase 2 isoform a [Homo sapiens] |
| GI:21361699 | RefSeq | XP_006723354.1 | 654 | PREDICTED: sphingosine kinase 2 isoform X2 [Homo sapiens] |
| GI:323462185 | RefSeq | XP_006723355.1 | 618 | PREDICTED: sphingosine kinase 2 isoform X3 [Homo sapiens] |
| GI:323462183 | RefSeq | XP_006723356.1 | 595 | PREDICTED: sphingosine kinase 2 isoform X4 [Homo sapiens] |
| GI:345197213 | RefSeq | XP_006723357.1 | 448 | PREDICTED: sphingosine kinase 2 isoform X5 [Homo sapiens] |
| GI:345197213 | RefSeq | XP_008964954.1 | 448 | PREDICTED: sphingosine kinase 2 isoform X4 [Pan paniscus] |
| GI:345197213 | RefSeq | XP_008964955.1 | 448 | PREDICTED: sphingosine kinase 2 isoform X4 [Pan paniscus] |
Related Sequences to LMP001959 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:21361699 | EMBL | CAB66636.1 | 654 | hypothetical protein [Homo sapiens] |
| GI:21361699 | GenBank | AAQ02408.1 | 655 | sphingosine kinase 2, partial [synthetic construct] |
| GI:21361699 | GenBank | EAW52365.1 | 672 | sphingosine kinase 2, isoform CRA_a [Homo sapiens] |
| GI:323462185 | GenBank | EAW52367.1 | 654 | sphingosine kinase 2, isoform CRA_c [Homo sapiens] |
| GI:345197213 | GenBank | EAW52367.1 | 654 | sphingosine kinase 2, isoform CRA_c [Homo sapiens] |
| GI:323462185 | GenBank | ABW03921.1 | 654 | sphingosine kinase 2 [synthetic construct] |
| GI:345197213 | GenBank | ABW03921.1 | 654 | sphingosine kinase 2 [synthetic construct] |
| GI:323462183 | GenBank | ABW03921.1 | 654 | sphingosine kinase 2 [synthetic construct] |
| GI:323462185 | GenBank | AED82287.1 | 654 | Sequence 1024 from patent US 7919467 |
| GI:345197213 | GenBank | AED82287.1 | 654 | Sequence 1024 from patent US 7919467 |
| GI:323462183 | GenBank | AED82287.1 | 654 | Sequence 1024 from patent US 7919467 |
| GI:21361699 | GenBank | JAA40122.1 | 654 | sphingosine kinase 2 [Pan troglodytes] |
| GI:345197213 | GenBank | AIC62777.1 | 654 | SPHK2, partial [synthetic construct] |
| GI:323462183 | GenBank | AIC62777.1 | 654 | SPHK2, partial [synthetic construct] |
| GI:323462185 | GenBank | AIC62777.1 | 654 | SPHK2, partial [synthetic construct] |
| GI:323462185 | RefSeq | NP_001191088.1 | 654 | sphingosine kinase 2 isoform a [Homo sapiens] |
| GI:323462183 | RefSeq | NP_001191088.1 | 654 | sphingosine kinase 2 isoform a [Homo sapiens] |
| GI:345197213 | RefSeq | NP_001191088.1 | 654 | sphingosine kinase 2 isoform a [Homo sapiens] |
| GI:21361699 | RefSeq | XP_003814128.1 | 654 | PREDICTED: sphingosine kinase 2 isoform X1 [Pan paniscus] |
| GI:21361699 | RefSeq | XP_003814129.1 | 654 | PREDICTED: sphingosine kinase 2 isoform X1 [Pan paniscus] |
| GI:323462183 | RefSeq | XP_003814131.1 | 595 | PREDICTED: sphingosine kinase 2 isoform X3 [Pan paniscus] |
| GI:345197213 | SwissProt | Q9NRA0.2 | 654 | RecName: Full=Sphingosine kinase 2; Short=SK 2; Short=SPK 2 [Homo sapiens] |
| GI:323462183 | SwissProt | Q9NRA0.2 | 654 | RecName: Full=Sphingosine kinase 2; Short=SK 2; Short=SPK 2 [Homo sapiens] |
| GI:323462185 | SwissProt | Q9NRA0.2 | 654 | RecName: Full=Sphingosine kinase 2; Short=SK 2; Short=SPK 2 [Homo sapiens] |