Gene/Proteome Database (LMPD)

LMPD ID
LMP002081
Gene ID
14595
Species
Mus musculus (Mouse)
Gene Name
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
Gene Symbol
B4galt1
Synonyms
B-1,4-GalT; B-1,4-GalT1; GalT; Ggtb; Ggtb2; b4Gal-T1; beta-1,4-GalT; beta-1,4-GalT1; beta4Gal-T1
Alternate Names
beta-1,4-galactosyltransferase 1; beta-1,4-GalTase 1; b1,4-Galactosyltransferase I; galactosyltransferase 2 beta 1, 4; glycoprotein galactosyltransferase beta 1, 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
Chromosome
4
Map Location
4 A5|4 20.46 cM
EC Number
2.4.1.-
Summary
This gene encodes two distinct enzyme isoforms, a long membrane-bound form and a short soluble form. These alternate isoforms are thought to be produced through alternative nested transcription initiation and different in-frame start codon usage. These enzymes catalyze the transfer of galactose to acceptor sugars, such as N-acetylglucosamine and glucose. The long form of this enzyme is localized to the trans-Golgi membrane and is involved in glycoconjugate biosynthesis. The short form functions in lactose biosynthesis though formation of a heterodimer with alpha-lactalbumin. [provided by RefSeq, Nov 2012]
Orthologs
View orthologs and multiple alignments for B4galt1

Proteins

beta-1,4-galactosyltransferase 1
Refseq ID NP_071641
Protein GI 11602910
UniProt ID P15535
mRNA ID NM_022305
Length 399
RefSeq Status REVIEWED
MRFREQFLGGSAAMPGATLQRACRLLVAVCALHLGVTLVYYLSGRDLSRLPQLVGVSSTLQGGTNGAAASKQPPGEQRPRGARPPPPLGVSPKPRPGLDSSPGAASGPGLKSNLSSLPVPTTTGLLSLPACPEESPLLVGPMLIDFNIAVDLELLAKKNPEIKTGGRYSPKDCVSPHKVAIIIPFRNRQEHLKYWLYYLHPILQRQQLDYGIYVINQAGDTMFNRAKLLNIGFQEALKDYDYNCFVFSDVDLIPMDDRNAYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQFLAINGFPNNYWGWGGEDDDIFNRLVHKGMSISRPNAVVGRCRMIRHSRDKKNEPNPQRFDRIAHTKETMRFDGLNSLTYKVLDVQRYPLYTQITVDIGTPR

Gene Information

Entrez Gene ID
14595
Gene Name
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
Gene Symbol
B4galt1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IDA:MGI C Golgi apparatus
GO:0000138 IEA:Ensembl C Golgi trans cisterna
GO:0016323 IEA:Ensembl C basolateral plasma membrane
GO:0031526 IEA:Ensembl C brush border membrane
GO:0009986 IDA:MGI C cell surface
GO:0030057 IEA:Ensembl C desmosome
GO:0009897 IEA:Ensembl C external side of plasma membrane
GO:0005615 IEA:Ensembl C extracellular space
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0030112 IEA:Ensembl C glycocalyx
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005886 IDA:MGI C plasma membrane
GO:0003945 IEA:UniProtKB-EC F N-acetyllactosamine synthase activity
GO:0003831 IDA:MGI F beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
GO:0008092 IDA:MGI F cytoskeletal protein binding
GO:0008378 IDA:MGI F galactosyltransferase activity
GO:0004461 IEA:UniProtKB-EC F lactose synthase activity
GO:0030145 ISS:UniProtKB F manganese ion binding
GO:0002526 IMP:MGI P acute inflammatory response
GO:0060055 IMP:MGI P angiogenesis involved in wound healing
GO:0007339 IMP:MGI P binding of sperm to zona pellucida
GO:0048754 IMP:MGI P branching morphogenesis of an epithelial tube
GO:0007155 IGI:MGI P cell adhesion
GO:0045136 IMP:MGI P development of secondary sexual characteristics
GO:0002064 IMP:MGI P epithelial cell development
GO:0030198 IMP:MGI P extracellular matrix organization
GO:0006012 IDA:MGI P galactose metabolic process
GO:0009101 IMP:MGI P glycoprotein biosynthetic process
GO:0005989 IMP:MGI P lactose biosynthetic process
GO:0050900 IMP:MGI P leukocyte migration
GO:0030879 IMP:MGI P mammary gland development
GO:0008285 IMP:MGI P negative regulation of cell proliferation
GO:0009312 IDA:MGI P oligosaccharide biosynthetic process
GO:0007341 IMP:MGI P penetration of zona pellucida
GO:0043065 IMP:MGI P positive regulation of apoptotic process
GO:0060058 IMP:MGI P positive regulation of apoptotic process involved in mammary gland involution
GO:0060054 IMP:MGI P positive regulation of epithelial cell proliferation involved in wound healing
GO:0006487 ISS:UniProtKB P protein N-linked glycosylation
GO:0006486 IMP:MGI P protein glycosylation
GO:0060046 IMP:MGI P regulation of acrosome reaction
GO:0042127 IMP:MGI P regulation of cell proliferation
GO:0051270 IDA:MGI P regulation of cellular component movement
GO:0042060 IMP:MGI P wound healing

KEGG Pathway Links

KEGG Pathway ID Description
mmu00533 Glycosaminoglycan biosynthesis - keratan sulfate
mmu00510 N-Glycan biosynthesis
mmu00514 Other types of O-glycan biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR003859 Beta-1,4-galactosyltransferase
IPR027791 Galactosyltransferase, C-terminal
IPR027995 Galactosyltransferase, N-terminal
IPR029044 Nucleotide-diphospho-sugar transferases

UniProt Annotations

Entry Information

Gene Name
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
Protein Entry
B4GT1_MOUSE
UniProt ID
P15535
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative initiation; Named isoforms=2; Name=Long; Synonyms=Cell surface; IsoId=P15535-1; Sequence=Displayed; Name=Short; Synonyms=Golgi complex; IsoId=P15535-2; Sequence=VSP_018803;
Catalytic Activity UDP-alpha-D-galactose + D-glucose = UDP + lactose.
Catalytic Activity UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Catalytic Activity UDP-alpha-D-galactose + N-acetyl-beta-D- glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N- acetyl-beta-D-glucosaminylglycopeptide.
Cofactor Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
Developmental Stage In the brain, highest levels of expression are found at 11.5 dpc with decreased expression thereafter. {ECO:0000269|PubMed:11145975}.
Function The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Function The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
Pathway Protein modification; protein glycosylation.
Ptm The soluble form derives from the membrane forms by proteolytic processing.
Similarity Belongs to the glycosyltransferase 7 family. {ECO:0000305}.
Subcellular Location Isoform Long: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Note=Found in trans cisternae of Golgi.
Subcellular Location Isoform Short: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Found in trans cisternae of Golgi.
Subcellular Location Processed beta-1,4-galactosyltransferase 1: Secreted. Note=Soluble form found in body fluids.
Subunit Homodimer; and heterodimer with alpha-lactalbumin to form lactose synthase.
Web Resource Name=Functional Glycomics Gateway - GTase; Note=b4GalT1; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_mou_460";

Identical and Related Proteins

Unique RefSeq proteins for LMP002081 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
11602910 RefSeq NP_071641 399 beta-1,4-galactosyltransferase 1

Identical Sequences to LMP002081 proteins

Reference Database Accession Length Protein Name
GI:11602910 DBBJ BAE32556.1 399 unnamed protein product [Mus musculus]
GI:11602910 DBBJ BAE21450.1 399 unnamed protein product [Mus musculus]
GI:11602910 GenBank ABK42529.1 399 betaGlcNAc beta 1,4-galactosyltransferase [synthetic construct]
GI:11602910 GenBank EDL05427.1 399 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1, isoform CRA_b [Mus musculus]
GI:11602910 GenBank ACN03420.1 399 Sequence 4 from patent US 7482133
GI:11602910 GenBank AFG79754.1 399 Sequence 13 from patent US 8137928

Related Sequences to LMP002081 proteins

Reference Database Accession Length Protein Name
GI:11602910 DBBJ BAE39424.1 386 unnamed protein product [Mus musculus]
GI:11602910 GenBank AAA58745.1 386 beta-1,4-galactosyltransferase [Mus musculus]
GI:11602910 GenBank EDL98643.1 399 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (mapped), isoform CRA_a [Rattus norvegicus]
GI:11602910 GenBank AGV95415.1 415 Sequence 3 from patent US 8512991
GI:11602910 RefSeq NP_445739.1 399 beta-1,4-galactosyltransferase 1 [Rattus norvegicus]
GI:11602910 RefSeq XP_005078916.1 399 PREDICTED: beta-1,4-galactosyltransferase 1 [Mesocricetus auratus]