Gene/Proteome Database (LMPD)

LMPD ID
LMP002105
Gene ID
239
Species
Homo sapiens (Human)
Gene Name
arachidonate 12-lipoxygenase
Gene Symbol
ALOX12
Synonyms
12-LOX; 12S-LOX; LOG12
Alternate Names
arachidonate 12-lipoxygenase, 12S-type; platelet 12-LOX; 12S-lipoxygenase; 12(S)-lipoxygenase; lipoxin synthase 12-LO; platelet-type 12-lipoxygenase; platelet-type lipoxygenase 12
Chromosome
17
Map Location
17p13.1
EC Number
1.13.11.31

Proteins

arachidonate 12-lipoxygenase, 12S-type
Refseq ID NP_000688
Protein GI 154426292
UniProt ID P18054
mRNA ID NM_000697
Length 663
RefSeq Status VALIDATED
MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLLQFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPLVMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNSDFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI

Gene Information

Entrez Gene ID
239
Gene Name
arachidonate 12-lipoxygenase
Gene Symbol
ALOX12
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:UniProtKB C cytoplasm
GO:0005829 IDA:UniProtKB C cytosol
GO:0070062 IDA:UniProt C extracellular vesicular exosome
GO:0016020 IDA:UniProtKB C membrane
GO:0042383 IDA:UniProtKB C sarcolemma
GO:0004052 IDA:UniProtKB F arachidonate 12-lipoxygenase activity
GO:0051120 TAS:Reactome F hepoxilin A3 synthase activity
GO:0047977 ISS:UniProtKB F hepoxilin-epoxide hydrolase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:0016165 IDA:UniProtKB F linoleate 13S-lipoxygenase activity
GO:0016702 TAS:Reactome F oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0007568 IEA:Ensembl P aging
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0006928 IMP:UniProtKB P cellular component movement
GO:0071396 IEA:Ensembl P cellular response to lipid
GO:0061436 ISS:UniProtKB P establishment of skin barrier
GO:0019395 IMP:UniProtKB P fatty acid oxidation
GO:0051122 ISS:UniProtKB P hepoxilin biosynthetic process
GO:0051121 TAS:Reactome P hepoxilin metabolic process
GO:1901751 IDA:UniProtKB P leukotriene A4 metabolic process
GO:0043651 IDA:UniProtKB P linoleic acid metabolic process
GO:2001303 IDA:UniProtKB P lipoxin A4 biosynthetic process
GO:2001306 IDA:UniProtKB P lipoxin B4 biosynthetic process
GO:2001300 TAS:Reactome P lipoxin metabolic process
GO:0019372 IDA:UniProtKB P lipoxygenase pathway
GO:0043066 IMP:UniProtKB P negative regulation of apoptotic process
GO:0010656 IMP:UniProtKB P negative regulation of muscle cell apoptotic process
GO:0090331 ISS:UniProtKB P negative regulation of platelet aggregation
GO:0045766 IMP:UniProtKB P positive regulation of angiogenesis
GO:0043065 IEA:Ensembl P positive regulation of apoptotic process
GO:0045785 IMP:UniProtKB P positive regulation of cell adhesion
GO:0030307 IMP:UniProtKB P positive regulation of cell growth
GO:0030335 IMP:UniProtKB P positive regulation of cell migration
GO:0008284 IMP:UniProtKB P positive regulation of cell proliferation
GO:0043280 IEA:Ensembl P positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0045603 IEA:Ensembl P positive regulation of endothelial cell differentiation
GO:0010595 IEA:Ensembl P positive regulation of endothelial cell migration
GO:0010628 IEA:Ensembl P positive regulation of gene expression
GO:0051901 IEA:Ensembl P positive regulation of mitochondrial depolarization
GO:0048661 IEA:Ensembl P positive regulation of smooth muscle cell proliferation
GO:0045909 IEA:Ensembl P positive regulation of vasodilation
GO:0072593 NAS:UniProtKB P reactive oxygen species metabolic process
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0042554 NAS:UniProtKB P superoxide anion generation

KEGG Pathway Links

KEGG Pathway ID Description
hsa04750 Inflammatory mediator regulation of TRP channels
hsa04726 Serotonergic synapse

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_150320 Synthesis of Lipoxins (LX)

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR020834 Lipoxygenase, conserved site
IPR013819 Lipoxygenase, C-terminal
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 12-lipoxygenase
Protein Entry
LOX12_HUMAN
UniProt ID
P18054
Species
Human

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=8 uM for arachidonate (PubMed:1851637, at pH 7.0 and 37 degrees Celsius) {ECO
Catalytic Activity (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa- 7,9,11,14-tetraenoate + H(2)O = (5S,14R,15S)-trihydroxy- (6E,8Z,10E,12E)-eicosatetraenoate.
Catalytic Activity (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa- 7,9,11,14-tetraenoate + H(2)O = (5S,6R,15S)-trihydroxy- (7E,9E,11Z,13E)-eicosatetraenoate.
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12S)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate.
Cofactor Name=Fe cation; Xref=ChEBI
Disease Colorectal cancer (CRC) [MIM
Disease Esophageal cancer (ESCR) [MIM
Enzyme Regulation Activated by EGF.
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. {ECO
Induction Down-regulated upon starvation, by UV-irradiation and 15-lipoxygenase metabolites. {ECO
Interaction P13647:KRT5; NbExp=7; IntAct=EBI-1633210, EBI-702187; P02545:LMNA; NbExp=4; IntAct=EBI-1633210, EBI-351935;
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Similarity Belongs to the lipoxygenase family.
Similarity Contains 1 lipoxygenase domain. {ECO
Similarity Contains 1 PLAT domain. {ECO
Subcellular Location Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF.
Tissue Specificity Expressed in vascular smooth muscle cells.
Web Resource Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/ALOX12ID620ch17p13.html";
Web Resource Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/alox12/";

Identical and Related Proteins

Unique RefSeq proteins for LMP002105 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
154426292 RefSeq NP_000688 663 arachidonate 12-lipoxygenase, 12S-type

Identical Sequences to LMP002105 proteins

Reference Database Accession Length Protein Name
GI:154426292 DBBJ BAJ20999.1 663 arachidonate 12-lipoxygenase, partial [synthetic construct]
GI:154426292 GenBank ABY15142.1 663 Sequence 517 from patent US 7306913
GI:154426292 GenBank ACP57370.1 663 Sequence 1499 from patent US 7482117
GI:154426292 GenBank ACP57371.1 663 Sequence 1500 from patent US 7482117
GI:154426292 GenBank ADS30967.1 663 Sequence 1499 from patent US 7781168
GI:154426292 GenBank ADS30968.1 663 Sequence 1500 from patent US 7781168

Related Sequences to LMP002105 proteins

Reference Database Accession Length Protein Name
GI:154426292 GenBank AAA59523.1 663 12-lipoxygenase [Homo sapiens]
GI:154426292 GenBank AAH69557.1 663 Arachidonate 12-lipoxygenase [Homo sapiens]
GI:154426292 GenBank AAY75403.1 663 Sequence 306 from patent US 6900016
GI:154426292 GenBank AAY75597.1 680 Sequence 500 from patent US 6900016
GI:154426292 GenBank AFO05690.1 663 Sequence 328 from patent US 8216786
GI:154426292 GenBank AHE01969.1 663 Sequence 59818 from patent US 8586006