Gene/Proteome Database (LMPD)

LMPD ID
LMP002227
Gene ID
18708
Species
Mus musculus (Mouse)
Gene Name
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
Gene Symbol
Pik3r1
Synonyms
PI3K; p50alpha; p55alpha; p85alpha
Alternate Names
phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
Chromosome
13
Map Location
13 D1|13 53.92 cM

Proteins

phosphatidylinositol 3-kinase regulatory subunit alpha isoform 1
Refseq ID NP_001020126
Protein GI 68299809
UniProt ID Q3TP23
mRNA ID NM_001024955
Length 454
RefSeq Status VALIDATED
MYTTVWTMEDLDLECAKTDINCGTDLVFYIEMDPPALPPKPPKPTTVANNSMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFNSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNHDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR
phosphatidylinositol 3-kinase regulatory subunit alpha isoform 2
Refseq ID NP_001070963
Protein GI 117320524
UniProt ID P26450
mRNA ID NM_001077495
Length 724
RefSeq Status VALIDATED
MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEDIGWLNGYNETTGERGDFPGTYVEYIGRKRISPPTPKPRPPRPLPVAPGSSKTEADTEQQALPLPDLAEQFAPPDVAPPLLIKLLEAIEKKGLECSTLYRTQSSSNPAELRQLLDCDAASVDLEMIDVHVLADAFKRYLADLPNPVIPVAVYNEMMSLAQELQSPEDCIQLLKKLIRLPNIPHQCWLTLQYLLKHFFKLSQASSKNLLNARVLSEIFSPVLFRFPAASSDNTEHLIKAIEILISTEWNERQPAPALPPKPPKPTTVANNSMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFNSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNHDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR

Gene Information

Entrez Gene ID
18708
Gene Name
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
Gene Symbol
Pik3r1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005911 IDA:MGI C cell-cell junction
GO:0005737 IDA:UniProtKB C cytoplasm
GO:0005829 IDA:UniProtKB C cytosol
GO:0016020 IDA:UniProtKB C membrane
GO:0005942 TAS:MGI C phosphatidylinositol 3-kinase complex
GO:0005943 ISS:UniProtKB C phosphatidylinositol 3-kinase complex, class IA
GO:0016303 TAS:MGI F 1-phosphatidylinositol-3-kinase activity
GO:0043125 ISS:UniProtKB F ErbB-3 class receptor binding
GO:0043559 IEA:Ensembl F insulin binding
GO:0005158 ISS:UniProtKB F insulin receptor binding
GO:0043560 IPI:UniProtKB F insulin receptor substrate binding
GO:0005159 ISS:UniProtKB F insulin-like growth factor receptor binding
GO:0035014 ISS:UniProtKB F phosphatidylinositol 3-kinase regulator activity
GO:0030183 IMP:MGI P B cell differentiation
GO:0051531 IMP:MGI P NFAT protein import into nucleus
GO:0034644 IMP:MGI P cellular response to UV
GO:0008625 IMP:MGI P extrinsic apoptotic signaling pathway via death domain receptors
GO:0060396 IEA:Ensembl P growth hormone receptor signaling pathway
GO:0008286 ISS:UniProtKB P insulin receptor signaling pathway
GO:0048009 ISS:UniProtKB P insulin-like growth factor receptor signaling pathway
GO:0008630 IMP:MGI P intrinsic apoptotic signaling pathway in response to DNA damage
GO:0043066 IDA:MGI P negative regulation of apoptotic process
GO:0001953 IGI:MGI P negative regulation of cell-matrix adhesion
GO:0045671 IGI:MGI P negative regulation of osteoclast differentiation
GO:0014065 IEA:Ensembl P phosphatidylinositol 3-kinase signaling
GO:0046854 ISS:UniProtKB P phosphatidylinositol phosphorylation
GO:0036092 TAS:GOC P phosphatidylinositol-3-phosphate biosynthetic process
GO:0030335 IGI:MGI P positive regulation of cell migration
GO:0045944 IMP:MGI P positive regulation of transcription from RNA polymerase II promoter
GO:0032760 IMP:MGI P positive regulation of tumor necrosis factor production
GO:0006468 IDA:MGI P protein phosphorylation
GO:0043551 ISS:GOC P regulation of phosphatidylinositol 3-kinase activity

KEGG Pathway Links

KEGG Pathway ID Description
mmu04152 AMPK signaling pathway
mmu04550 Signaling pathways regulating pluripotency of stem cells
mmu04024 cAMP signaling pathway
mmu04022 cGMP-PKG signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR001720 PI3 kinase, P85 regulatory subunit
IPR008936 Rho GTPase activation protein
IPR000198 Rho GTPase-activating protein domain
IPR000980 SH2 domain
IPR001452 SH3_domain
IPR011511 Variant SH3 domain

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
Protein Entry
Q3TP23_MOUSE
UniProt ID
Q3TP23
Species
Mouse

Comments

Comment Type Description
Domain The SH3 domain mediates the binding to CBLB. {ECO:0000250}.
Function Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). {ECO:0000250}.
Interaction Q8IZP0:ABI1 (xeno); NbExp=3; IntAct=EBI-641764, EBI-375446; O88665:Brd7; NbExp=11; IntAct=EBI-641764, EBI-643930; Q62448:Eif4g2; NbExp=3; IntAct=EBI-641764, EBI-296494; P35569:Irs1; NbExp=2; IntAct=EBI-641764, EBI-400825; P42337:Pik3ca; NbExp=6; IntAct=EBI-641764, EBI-641748; Q8BTI9:Pik3cb; NbExp=4; IntAct=EBI-641764, EBI-644672; O35904-2:Pik3cd; NbExp=2; IntAct=EBI-641764, EBI-6470774; Q8R5H6:Wasf1; NbExp=2; IntAct=EBI-641764, EBI-774719;
Ptm Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR (By similarity). Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. {ECO:0000250, ECO:0000269|PubMed:10353604, ECO:0000269|PubMed:10521483, ECO:0000269|PubMed:11294897, ECO:0000269|PubMed:17947660, ECO:0000269|PubMed:18034455, ECO:0000269|PubMed:9312046}.
Ptm Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. {ECO:0000269|PubMed:11526404}.
Similarity Belongs to the PI3K p85 subunit family. {ECO:0000305}.
Similarity Contains 1 Rho-GAP domain. {ECO:0000255|PROSITE- ProRule:PRU00172}.
Similarity Contains 1 SH3 domain. {ECO:0000255|PROSITE- ProRule:PRU00192}.
Similarity Contains 2 SH2 domains. {ECO:0000255|PROSITE- ProRule:PRU00191}.
Subunit Heterodimer of a regulatory subunit PIK3R1 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with IRS1 and phosphorylated IRS4. Interacts with NISCH and RUFY3 (By similarity). Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR or BCR activation. Interacts with CBLB. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with SOCS7 and HCST. Interacts with AXL, FASLG, FGR, HCK, KIT and BCR. Interacts with PTK2/FAK1 (By similarity). Interacts with PDGFRB (tyrosine phosphorylated) (By similarity). Interacts with NTRK1 (phosphorylated upon ligand- binding) (By similarity). Interacts with FER. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated) (Probable). Interacts with PDGFRA (tyrosine phosphorylated). Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts with LYN (via SH3 domain); this enhances enzyme activity. Interacts with ERBB4. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). {ECO:0000250, ECO:0000269|PubMed:10353604, ECO:0000269|PubMed:10521483, ECO:0000269|PubMed:10528161, ECO:0000269|PubMed:10646608, ECO:0000269|PubMed:11006284, ECO:0000269|PubMed:11113178, ECO:0000269|PubMed:11294897, ECO:0000269|PubMed:11526404, ECO:0000269|PubMed:11711534, ECO:0000269|PubMed:12052866, ECO:0000269|PubMed:14610044, ECO:0000269|PubMed:16249387, ECO:0000269|PubMed:1646396, ECO:0000269|PubMed:8128248, ECO:0000269|PubMed:8943348, ECO:0000269|PubMed:9312046, ECO:0000305}.

Identical and Related Proteins

Unique RefSeq proteins for LMP002227 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
68299809 RefSeq NP_001020126 454 phosphatidylinositol 3-kinase regulatory subunit alpha isoform 1
117320524 RefSeq NP_001070963 724 phosphatidylinositol 3-kinase regulatory subunit alpha isoform 2

Identical Sequences to LMP002227 proteins

Reference Database Accession Length Protein Name
GI:117320524 GenBank AAH26146.1 724 Phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) [Mus musculus]
GI:68299809 GenBank AAH51106.1 454 Phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) [Mus musculus]
GI:117320524 GenBank ABK42316.1 724 phosphoinositide 3-kinase p85 alpha regulatory subunit [synthetic construct]
GI:117320524 GenBank EDL00772.1 724 phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) [Mus musculus]
GI:117320524 SwissProt P26450.2 724 RecName: Full=Phosphatidylinositol 3-kinase regulatory subunit alpha; Short=PI3-kinase regulatory subunit alpha; Short=PI3K regulatory subunit alpha; Short=PtdIns-3-kinase regulatory subunit alpha; AltName: Full=Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Short=PI3-kinase subunit p85-alpha; Short=PtdIns-3-kinase regulatory subunit p85-alpha [Mus musculus]

Related Sequences to LMP002227 proteins

Reference Database Accession Length Protein Name
GI:68299809 DBBJ BAA18933.1 454 phosphatidylinositol 3-kinase p45 subunit [Rattus norvegicus]
GI:117320524 EMBL CAD90931.1 724 unnamed protein product [Mus musculus]
GI:117320524 GenBank AAA39886.1 724 phosphoprotein p85 [Mus musculus domesticus]
GI:117320524 GenBank AAC52847.1 724 phosphoinositide 3-kinase p85alpha [Mus musculus]
GI:117320524 GenBank AAQ80266.1 722 Sequence 12 from patent US 6300111
GI:68299809 GenBank EDM10232.1 454 rCG44396, isoform CRA_a [Rattus norvegicus]
GI:117320524 GenBank EDM10233.1 724 rCG44396, isoform CRA_b [Rattus norvegicus]
GI:68299809 GenBank ERE82807.1 674 phosphatidylinositol 3-kinase regulatory subunit alpha-like isoform 1 [Cricetulus griseus]
GI:117320524 RefSeq XP_005065653.1 724 PREDICTED: phosphatidylinositol 3-kinase regulatory subunit alpha isoform X1 [Mesocricetus auratus]
GI:68299809 RefSeq XP_005065654.1 454 PREDICTED: phosphatidylinositol 3-kinase regulatory subunit alpha isoform X2 [Mesocricetus auratus]
GI:68299809 RefSeq XP_006231930.1 454 PREDICTED: phosphatidylinositol 3-kinase regulatory subunit alpha isoform X1 [Rattus norvegicus]
GI:68299809 RefSeq XP_006982786.1 454 PREDICTED: phosphatidylinositol 3-kinase regulatory subunit alpha isoform X2 [Peromyscus maniculatus bairdii]