Gene/Proteome Database (LMPD)
LMPD ID
LMP002363
Gene ID
Species
Homo sapiens (Human)
Gene Name
cytochrome P450, family 4, subfamily F, polypeptide 3
Gene Symbol
Synonyms
CPF3; CYP4F; LTB4H
Alternate Names
docosahexaenoic acid omega-hydroxylase CYP4F3; CYPIVF3; 20-HETE synthase; cytochrome P-450; cytochrome P450 4F3; cytochrome P450-LTB-omega; leukotriene-B4 20-monooxygenase; leukotriene B4 omega hydroxylase; leukotriene-B(4) 20-monooxygenase 2; leukotriene-B(4) omega-hydroxylase 2; 20-hydroxyeicosatetraenoic acid synthase; cytochrome P450, subfamily IVF, polypeptide 3 (leukotriene B4 omega hydroxylase)
Chromosome
19
Map Location
19p13.2
EC Number
1.14.13.199
Summary
This gene, CYP4F3, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F8, is approximately 18 kb away. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Orthologs
Proteins
| leukotriene-B(4) omega-hydroxylase 2 isoform a | |
|---|---|
| Refseq ID | NP_000887 |
| Protein GI | 119220562 |
| UniProt ID | Q08477 |
| mRNA ID | NM_000896 |
| Length | 520 |
| RefSeq Status | REVIEWED |
| MPQLSLSSLGLWPMAASPWLLLLLVGASWLLARILAWTYTFYDNCCRLRCFPQPPKRNWFLGHLGLIHSSEEGLLYTQSLACTFGDMCCWWVGPWHAIVRIFHPTYIKPVLFAPAAIVPKDKVFYSFLKPWLGDGLLLSAGEKWSRHRRMLTPAFHFNILKPYMKIFNESVNIMHAKWQLLASEGSARLDMFEHISLMTLDSLQKCVFSFDSHCQEKPSEYIAAILELSALVTKRHQQILLYIDFLYYLTPDGQRFRRACRLVHDFTDAVIQERRRTLPSQGVDDFLQAKAKSKTLDFIDVLLLSKDEDGKKLSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQERCRQEVQELLKDREPKEIEWDDLAQLPFLTMCIKESLRLHPPVPAVSRCCTQDIVLPDGRVIPKGIICLISVFGTHHNPAVWPDPEVYDPFRFDPKNIKERSPLAFIPFSAGPRNCIGQAFAMAEMKVVLGLTLLRFRVLPDHTEPRRKPELVLRAEGGLWLRVEPLS | |
| leukotriene-B(4) omega-hydroxylase 2 isoform b | |
|---|---|
| Refseq ID | NP_001186137 |
| Protein GI | 312836833 |
| UniProt ID | Q08477 |
| mRNA ID | NM_001199208 |
| Length | 520 |
| RefSeq Status | REVIEWED |
| MPQLSLSSLGLWPMAASPWLLLLLVGASWLLARILAWTYTFYDNCCRLRCFPQPPKRNWFLGHLGLVTPTEQGMRVLTQLVATYPQGFKVWMGPIFPVIRFCHPNIIRSVINASAAIVPKDKVFYSFLKPWLGDGLLLSAGEKWSRHRRMLTPAFHFNILKPYMKIFNESVNIMHAKWQLLASEGSARLDMFEHISLMTLDSLQKCVFSFDSHCQEKPSEYIAAILELSALVTKRHQQILLYIDFLYYLTPDGQRFRRACRLVHDFTDAVIQERRRTLPSQGVDDFLQAKAKSKTLDFIDVLLLSKDEDGKKLSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQERCRQEVQELLKDREPKEIEWDDLAQLPFLTMCIKESLRLHPPVPAVSRCCTQDIVLPDGRVIPKGIICLISVFGTHHNPAVWPDPEVYDPFRFDPKNIKERSPLAFIPFSAGPRNCIGQAFAMAEMKVVLGLTLLRFRVLPDHTEPRRKPELVLRAEGGLWLRVEPLS | |
| leukotriene-B(4) omega-hydroxylase 2 isoform b | |
|---|---|
| Refseq ID | NP_001186138 |
| Protein GI | 312836835 |
| UniProt ID | Q08477 |
| mRNA ID | NM_001199209 |
| Length | 520 |
| RefSeq Status | REVIEWED |
| Protein sequence is identical to GI:312836833 (mRNA isoform) | |
Gene Information
Entrez Gene ID
Gene Name
cytochrome P450, family 4, subfamily F, polypeptide 3
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005789 | TAS:Reactome | C | endoplasmic reticulum membrane |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0052871 | IEA:UniProtKB-EC | F | alpha-tocopherol omega-hydroxylase activity |
| GO:0020037 | IEA:InterPro | F | heme binding |
| GO:0005506 | IEA:InterPro | F | iron ion binding |
| GO:0050051 | IEA:UniProtKB-EC | F | leukotriene-B4 20-monooxygenase activity |
| GO:0004497 | TAS:ProtInc | F | monooxygenase activity |
| GO:0019369 | TAS:Reactome | P | arachidonic acid metabolic process |
| GO:0006690 | TAS:Reactome | P | icosanoid metabolic process |
| GO:0006691 | TAS:Reactome | P | leukotriene metabolic process |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0006805 | TAS:Reactome | P | xenobiotic metabolic process |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_13645 | Eicosanoids |
| REACT_13425 | Miscellaneous substrates |
| REACT_150420 | Synthesis of Leukotrienes (LT) and Eoxins (EX) |
Domain Information
UniProt Annotations
Entry Information
Gene Name
cytochrome P450, family 4, subfamily F, polypeptide 3
Protein Entry
CP4F3_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative promoter usage, Alternative splicing; Named isoforms=2; Name=CYP4F3A; IsoId=Q08477-1; Sequence=Displayed; Name=CYP4F3B; IsoId=Q08477-2; Sequence=VSP_047193; |
| Catalytic Activity | (5Z,8Z,11Z,14Z)-icosatetraenoate + NADPH + O(2) = (5Z,8Z,11Z,14Z)-20-hydroxyicosa-5,8,11,14-tetraenoate + NADP(+) + H(2)O. {ECO |
| Catalytic Activity | (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa- 6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)- 5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O. |
| Catalytic Activity | Docosahexaenoate + NADPH + O(2) = 22- hydroxydocosahexaenoate + NADP(+) + H(2)O. |
| Cofactor | Name=heme; Xref=ChEBI |
| Enzyme Regulation | Inhibited by carbon monoxide (CO). |
| Function | Isoform CYP4F3A |
| Function | Isoform CYP4F3B |
| Pathway | Lipid metabolism; leukotriene B4 degradation. |
| Similarity | Belongs to the cytochrome P450 family. |
| Subcellular Location | Endoplasmic reticulum membrane {ECO |
| Tissue Specificity | Isoform CYP4F3A is expressed in the polymorphonuclear leukocytes as well as leukocytes and bone marrow. Isoform CYP4F3B is selectively expressed in liver and kidney and is also the predominant CYP4F isoform in trachea and tissues of the gastrointestinal tract. |
| Web Resource | Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/cyp4f3/"; |
Identical and Related Proteins
Unique RefSeq proteins for LMP002363 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 119220562 | RefSeq | NP_000887 | 520 | leukotriene-B(4) omega-hydroxylase 2 isoform a |
| 312836833 | RefSeq | NP_001186137 | 520 | leukotriene-B(4) omega-hydroxylase 2 isoform b |
Identical Sequences to LMP002363 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:312836833 | DBBJ | BAH14129.1 | 520 | unnamed protein product [Homo sapiens] |
| GI:312836833 | DBBJ | BAH14507.1 | 520 | unnamed protein product [Homo sapiens] |
| GI:119220562 | GenBank | AAV40834.1 | 520 | cytochrome P450, family 4, subfamily F, polypeptide 3 [Homo sapiens] |
| GI:119220562 | GenBank | EAW84488.1 | 520 | cytochrome P450, family 4, subfamily F, polypeptide 3, isoform CRA_a [Homo sapiens] |
| GI:312836833 | GenBank | EAW84489.1 | 520 | cytochrome P450, family 4, subfamily F, polypeptide 3, isoform CRA_b [Homo sapiens] |
| GI:119220562 | GenBank | EAW84490.1 | 520 | cytochrome P450, family 4, subfamily F, polypeptide 3, isoform CRA_a [Homo sapiens] |
| GI:312836833 | GenBank | EAW84491.1 | 520 | cytochrome P450, family 4, subfamily F, polypeptide 3, isoform CRA_b [Homo sapiens] |
| GI:312836833 | GenBank | AAI36300.1 | 520 | CYP4F3 protein [Homo sapiens] |
| GI:312836833 | RefSeq | NP_001186138.1 | 520 | docosahexaenoic acid omega-hydroxylase CYP4F3 isoform b [Homo sapiens] |
| GI:119220562 | SwissProt | Q08477.2 | 520 | RecName: Full=Docosahexaenoic acid omega-hydroxylase CYP4F3; AltName: Full=20-hydroxyeicosatetraenoic acid synthase; Short=20-HETE synthase; AltName: Full=CYPIVF3; AltName: Full=Cytochrome P450 4F3; AltName: Full=Cytochrome P450-LTB-omega; AltName: Full=Leukotriene-B(4) 20-monooxygenase 2; AltName: Full=Leukotriene-B(4) omega-hydroxylase 2 [Homo sapiens] |
Related Sequences to LMP002363 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:119220562 | DBBJ | BAA02144.1 | 520 | cytochrome P-450LTBV [Homo sapiens] |
| GI:119220562 | DBBJ | BAA25990.1 | 520 | leukotriene B4 omega-hydroxylase [Homo sapiens] |
| GI:119220562 | DBBJ | BAA25991.1 | 520 | leukotriene B4 omega-hydroxylase [Homo sapiens] |
| GI:312836833 | GenBank | AAC08589.1 | 520 | cytochrome P-450 [Homo sapiens] |
| GI:119220562 | GenBank | ACM80505.1 | 520 | Sequence 6003 from patent US 6812339 |
| GI:119220562 | GenBank | ACM84580.1 | 527 | Sequence 10078 from patent US 6812339 |
| GI:119220562 | GenBank | AHD78003.1 | 520 | Sequence 24982 from patent US 8586006 |
| GI:312836833 | RefSeq | XP_003275960.1 | 520 | PREDICTED: leukotriene-B(4) omega-hydroxylase 2-like isoform 2 [Nomascus leucogenys] |
| GI:312836833 | RefSeq | XP_001159781.2 | 520 | PREDICTED: leukotriene-B(4) omega-hydroxylase 1 isoform X1 [Pan troglodytes] |
| GI:312836833 | RefSeq | XP_003813621.1 | 520 | PREDICTED: leukotriene-B(4) omega-hydroxylase 1 isoform X1 [Pan paniscus] |
| GI:312836833 | RefSeq | XP_004060254.1 | 520 | PREDICTED: leukotriene-B(4) omega-hydroxylase 1-like [Gorilla gorilla gorilla] |
| GI:312836833 | RefSeq | XP_009433197.1 | 520 | PREDICTED: leukotriene-B(4) omega-hydroxylase 1 isoform X1 [Pan troglodytes] |