Gene/Proteome Database (LMPD)

LMPD ID
LMP002387
Gene ID
2923
Species
Homo sapiens (Human)
Gene Name
protein disulfide isomerase family A, member 3
Gene Symbol
PDIA3
Synonyms
ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; P58; PI-PLC
Alternate Names
protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n
Chromosome
15
Map Location
15q15
EC Number
5.3.4.1
Summary
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for PDIA3

Proteins

protein disulfide-isomerase A3 precursor
Refseq ID NP_005304
Protein GI 21361657
UniProt ID P30101
mRNA ID NM_005313
Length 505
RefSeq Status REVIEWED
MRLRRLALFPGVALLLAAARLAAASDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL
sig_peptide: 1..24 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2535 peptide sequence: MRLRRLALFPGVALLLAAARLAAA mat_peptide: 25..505 product: protein disulfide-isomerase A3 calculated_mol_wt: 54265 peptide sequence: SDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL

Gene Information

Entrez Gene ID
2923
Gene Name
protein disulfide isomerase family A, member 3
Gene Symbol
PDIA3
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009986 IDA:MGI C cell surface
GO:0005783 IDA:UniProtKB C endoplasmic reticulum
GO:0005788 TAS:Reactome C endoplasmic reticulum lumen
GO:0070062 IDA:UniProt C extracellular vesicular exosome
GO:0005925 IDA:UniProtKB C focal adhesion
GO:0005634 IDA:UniProt C nucleus
GO:0004197 TAS:ProtInc F cysteine-type endopeptidase activity
GO:0004629 TAS:ProtInc F phospholipase C activity
GO:0044822 IDA:UniProtKB F poly(A) RNA binding
GO:0003756 IBA:RefGenome F protein disulfide isomerase activity
GO:0042590 TAS:Reactome P antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0002479 TAS:Reactome P antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
GO:0002474 TAS:Reactome P antigen processing and presentation of peptide antigen via MHC class I
GO:0045454 IEA:InterPro P cell redox homeostasis
GO:0044267 TAS:Reactome P cellular protein metabolic process
GO:2001238 IEA:Ensembl P positive regulation of extrinsic apoptotic signaling pathway
GO:0043687 TAS:Reactome P post-translational protein modification
GO:0018279 TAS:Reactome P protein N-linked glycosylation via asparagine
GO:0006457 IBA:RefGenome P protein folding
GO:0006606 TAS:ProtInc P protein import into nucleus
GO:0006621 TAS:ProtInc P protein retention in ER lumen
GO:0006508 TAS:GOC P proteolysis
GO:0034976 IBA:RefGenome P response to endoplasmic reticulum stress
GO:0007165 TAS:ProtInc P signal transduction

KEGG Pathway Links

KEGG Pathway ID Description
hsa04612 Antigen processing and presentation
hsa04141 Protein processing in endoplasmic reticulum

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_75795 Antigen Presentation: Folding, assembly and peptide loading of class I MHC
REACT_111178 ER-Phagosome pathway

Domain Information

InterPro Annotations

Accession Description
IPR005788 Disulphide_isomerase
IPR005792 Protein disulphide isomerase
IPR017937 Thioredoxin, conserved site
IPR012336 Thioredoxin-like fold
IPR013766 Thioredoxin_domain

UniProt Annotations

Entry Information

Gene Name
protein disulfide isomerase family A, member 3
Protein Entry
PDIA3_HUMAN
UniProt ID
P30101
Species
Human

Comments

Comment Type Description
Catalytic Activity Catalyzes the rearrangement of -S-S- bonds in proteins.
Caution Was originally thought to be a phosphatidylinositol 4,5- bisphosphate phosphodiesterase type I (phospholipase C-alpha).
Interaction P05067:APP; NbExp=3; IntAct=EBI-979862, EBI-77613; P18418:Calr (xeno); NbExp=2; IntAct=EBI-979862, EBI-916742; Q96HE7:ERO1L; NbExp=3; IntAct=EBI-979862, EBI-2564539; Q86YB8:ERO1LB; NbExp=2; IntAct=EBI-979862, EBI-2806988; Q13162:PRDX4; NbExp=2; IntAct=EBI-979862, EBI-2211957; Q13586:STIM1; NbExp=3; IntAct=EBI-979862, EBI-448878; Q03518:TAP1; NbExp=4; IntAct=EBI-979862, EBI-747259;
Mass Spectrometry Mass=54265.22; Method=MALDI; Range=25-505; Evidence= ;
Similarity Belongs to the protein disulfide isomerase family.
Similarity Contains 2 thioredoxin domains. {ECO
Subcellular Location Endoplasmic reticulum. Endoplasmic reticulum lumen . Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Subunit Subunit of the TAP complex, also known as the peptide loading complex (PLC). Can form disulfide-linked heterodimers with TAPBP. Interacts with ERP27 and CANX. Interacts with SERPINA2 and with the S and Z variants of SERPINA1. {ECO
Tissue Specificity Detected in the flagellum and head region of spermatozoa (at protein level).

Identical and Related Proteins

Unique RefSeq proteins for LMP002387 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
21361657 RefSeq NP_005304 505 protein disulfide-isomerase A3 precursor

Identical Sequences to LMP002387 proteins

Reference Database Accession Length Protein Name
GI:21361657 GenBank AFL39179.1 505 Sequence 12 from patent US 8192985
GI:21361657 GenBank JAA21928.1 505 protein disulfide isomerase family A, member 3 [Pan troglodytes]
GI:21361657 GenBank AGM91839.1 505 Sequence 12 from patent US 8409857
GI:21361657 GenBank AIC48892.1 505 PDIA3, partial [synthetic construct]
GI:21361657 GenBank AIC54505.1 505 PDIA3, partial [synthetic construct]
GI:21361657 RefSeq XP_003814998.1 505 PREDICTED: protein disulfide-isomerase A3 [Pan paniscus]

Related Sequences to LMP002387 proteins

Reference Database Accession Length Protein Name
GI:21361657 DBBJ BAA11928.1 505 ER-60 protease [Homo sapiens]
GI:21361657 GenBank AAC51518.1 505 ER-60 protein [Homo sapiens]
GI:21361657 GenBank AAP36370.1 506 Homo sapiens glucose regulated protein, 58kDa, partial [synthetic construct]
GI:21361657 GenBank AAX29670.1 506 glucose regulated protein 58kDa, partial [synthetic construct]
GI:21361657 GenBank AAX29671.1 506 glucose regulated protein 58kDa, partial [synthetic construct]
GI:21361657 GenBank AEN34022.1 505 Sequence 2 from patent US 7998508