Gene/Proteome Database (LMPD)

LMPD ID
LMP002394
Gene ID
67331
Species
Mus musculus (Mouse)
Gene Name
ATPase, class I, type 8B, member 3
Gene Symbol
Atp8b3
Synonyms
1700042F02Rik; 1700056N23Rik; ATPIK; SAPLT
Alternate Names
phospholipid-transporting ATPase IK; sperm aminophospholipid transporter; spermatozoan aminophospholipid translocase
Chromosome
10
Map Location
10 C1|10
EC Number
3.6.3.1

Proteins

phospholipid-transporting ATPase IK
Refseq ID NP_080370
Protein GI 46358405
UniProt ID Q6UQ17
mRNA ID NM_026094
Length 1335
RefSeq Status PROVISIONAL
MDGVHLGENLEDKDTEFTWEVKANDRTYHKQFKKKGFLCWRQKKYKSNAIHTAKYNIFSFLPLNLYEQFHRMSNLYFLFIIILQGIPEISTLPWFTLFAPLVCLFVIRATRDLVDDIGRHRSDKIINNRPCQILRGKSFLWKKWKNLCVGDVVCLSKDSIVPADLLLLASTEPSSLCYVETADIDGETNLKFRQALTVTHHELTSPKKMASFQGTVTCEEPNSRMHHFVGSLEWNSRKYPLDIGNLLLRGCKIRNTDTCYGLVIYAGLDTKIMKNCGKIHLKRTKLDLMMNKLVALIFLSLVIASLLLTVGFTFMVKQFKAKHYYMSPTHGRSDAMESFFIFWGFLILLSVMVPMAMFIIAEFIYLGNSIFINWDLNMYYEPLDMPAKARSTSLNDQLGQVQYIFSDKTGTLTQNIMTFKKCCINGCIYDSDDEHGTLRKRNPYAWNPFADGKLQFYNKELESLVQGRQDRAVQEFWRLLAICHTVMVQEKDNQLLYQAASPDEEALVTAARNFGYVFLSRTQDTITLVELGEERVYQVLAMMDFNSVRKRMSVLVRNPEGSICLYTKGADTVILERLRSKGVMEATTEEVLAAFAEQTLRTLCLAYKDVEEDAYKEWEPEHQEAALLLQNRAQALHQVYNKMEQNLQLLGATAIEDKLQDGVPETIKCLKKGNIKIWVLTGDKPETAVNIGFACQLLSENMIILEDKDINQVLERYWEDNVHQKAFKMMTHHNMALVINGEFLDQLLLSLRKEPRALVQNAVVDEVAQEPVVSALDFLQKRRISQMWRNAGPSLGTSHSADSKIRESPEVQRERAFVDLASKCQAVICCRVTPKQKALVVALVKKYQQVVTLAIGDGANDVNMIKTADIGVGLAGQEGMQAVQNSDYVLAQFCYLQRLLLVHGRWSYMRVCKFLRYFFYKTVASMMAQIWFSLVNGFSAQPLYEGWFLALFNLLYSTLPVLYIGLFEQDVTAEKSLKMPELYMAGQKGELFNYSIFMQAITHGTITSMINFFVTVMVSSDMSKAGSSHDYQSLGVLVAISSLLSVTLEVMLVVKYWTLLFVGAVVLSLSSYVLMTSLTQSLWMYRISPKTFPFLFADYNVLFEPCSLLLIVLNVALNVLPMLALRTIHRTVLKQRPKGEEEAPSEEVAVEPAMRHLRRGIPARRSSYAFSHREGYANLITQGTILRRQTHVDDSDGGTVCESLNPPEEDIPLQNKDSVFNPRKISILAKKRRHFFGKGSQEEVHPNTSSQTMEKQPTIHRDSETQKLPTTTSATSGKLLPSASEDEAFYSVASQYTLASQPKHTDVHSSFWKSPLWRDSASSSPSQLEVPRKQS

Gene Information

Entrez Gene ID
67331
Gene Name
ATPase, class I, type 8B, member 3
Gene Symbol
Atp8b3
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0001669 IDA:MGI C acrosomal vesicle
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IDA:MGI C integral component of membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0019829 IEA:InterPro F cation-transporting ATPase activity
GO:0000287 IEA:InterPro F magnesium ion binding
GO:0004012 IMP:MGI F phospholipid-translocating ATPase activity
GO:0007339 IMP:MGI P binding of sperm to zona pellucida
GO:0045332 IMP:MGI P phospholipid translocation

Domain Information

InterPro Annotations

Accession Description
IPR008250 ATPase_P-typ_transduc_dom_A
IPR001757 Cation_transp_P_typ_ATPase
IPR023214 HAD-like domain
IPR023299 P-type ATPase, cytoplasmic domain N
IPR018303 P-type ATPase, phosphorylation site
IPR006539 P-type ATPase, subfamily IV

UniProt Annotations

Entry Information

Gene Name
ATPase, class I, type 8B, member 3
Protein Entry
AT8B3_MOUSE
UniProt ID
Q6UQ17
Species
Mouse

Comments

Comment Type Description
Catalytic Activity ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).
Developmental Stage Developmentally regulated in testis. Expression is first detected at 17 days after birth and is later up-regulated up to adulthood amd maintained thereafter. {ECO:0000269|PubMed:19017724}.
Disruption Phenotype Disrupted normal fertilization by sperm; sensitivity to sperm concentration and the time required to fertilize an egg is increased; the number of spermatozoa bound to zona pellucida is decreased. {ECO:0000269|PubMed:14975727}.
Function P4-ATPase flippase which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May be responsible for the maintenance of asymmetric distribution of phosphatidylserine (PS) in spermatozoa membranes. Involved in acrosome reactions and binding of spermatozoa to zona pellucida. {ECO:0000269|PubMed:14975727}.
Similarity Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. {ECO:0000305}.
Subcellular Location Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Multi-pass membrane protein. Endoplasmic reticulum {ECO:0000250}.
Tissue Specificity Expressed in testis, specifically in spermatids within seminiferous tubules (at protein level). {ECO:0000269|PubMed:14975727, ECO:0000269|PubMed:19017724}.

Identical and Related Proteins

Unique RefSeq proteins for LMP002394 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
46358405 RefSeq NP_080370 1335 phospholipid-transporting ATPase IK

Identical Sequences to LMP002394 proteins

Reference Database Accession Length Protein Name
GI:46358405 GenBank AAR12913.1 1335 SAPLT [Mus musculus]
GI:46358405 GenBank AAI18978.1 1335 ATPase, class I, type 8B, member 3 [Mus musculus]
GI:46358405 SwissProt Q6UQ17.1 1335 RecName: Full=Phospholipid-transporting ATPase IK; AltName: Full=ATPase, class I, type 8B, member 3; AltName: Full=Sperm aminophospholipid transporter; Short=SAPLT [Mus musculus]

Related Sequences to LMP002394 proteins

Reference Database Accession Length Protein Name
GI:46358405 GenBank EDL31538.1 1309 mCG13368, isoform CRA_a, partial [Mus musculus]
GI:46358405 GenBank ERE72428.1 1332 putative phospholipid-transporting ATPase IK-like protein [Cricetulus griseus]
GI:46358405 RefSeq XP_234937.3 1340 PREDICTED: phospholipid-transporting ATPase IK [Rattus norvegicus]
GI:46358405 RefSeq XP_001076355.1 1340 PREDICTED: phospholipid-transporting ATPase IK [Rattus norvegicus]
GI:46358405 RefSeq XP_005359103.1 1335 PREDICTED: probable phospholipid-transporting ATPase IK [Microtus ochrogaster]
GI:46358405 RefSeq XP_007641392.1 1332 PREDICTED: probable phospholipid-transporting ATPase IK isoform X1 [Cricetulus griseus]