Gene/Proteome Database (LMPD)
LMPD ID
LMP002433
Gene ID
Species
Homo sapiens (Human)
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
Synonyms
12R-LOX; ARCI2
Alternate Names
arachidonate 12-lipoxygenase, 12R-type; 12R-lipoxygenase; epidermis-type lipoxygenase 12
Chromosome
17
Map Location
17p13.1
EC Number
1.13.11.-
Summary
This gene encodes an enzyme involved in the converstion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| arachidonate 12-lipoxygenase, 12R-type | |
|---|---|
| Refseq ID | NP_001130 |
| Protein GI | 4502053 |
| UniProt ID | O75342 |
| mRNA ID | NM_001139 |
| Length | 701 |
| RefSeq Status | REVIEWED |
| MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQDLGELIIIRLHKERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSISI | |
Gene Information
Entrez Gene ID
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | TAS:Reactome | C | cytosol |
| GO:0004052 | IMP:UniProtKB | F | arachidonate 12-lipoxygenase activity |
| GO:0005506 | IEA:InterPro | F | iron ion binding |
| GO:1990136 | ISS:UniProtKB | F | linoleate 9S-lipoxygenase activity |
| GO:0016702 | IDA:UniProtKB | F | oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen |
| GO:0019369 | IMP:UniProtKB | P | arachidonic acid metabolic process |
| GO:0046513 | ISS:UniProtKB | P | ceramide biosynthetic process |
| GO:0061436 | ISS:UniProtKB | P | establishment of skin barrier |
| GO:0051122 | ISS:UniProtKB | P | hepoxilin biosynthetic process |
| GO:0043651 | ISS:UniProtKB | P | linoleic acid metabolic process |
| GO:0019372 | IDA:UniProtKB | P | lipoxygenase pathway |
| GO:0055114 | IDA:UniProtKB | P | oxidation-reduction process |
| GO:0043410 | IDA:UniProtKB | P | positive regulation of MAPK cascade |
| GO:0010628 | IMP:UniProtKB | P | positive regulation of gene expression |
| GO:0070257 | IMP:UniProtKB | P | positive regulation of mucus secretion |
| GO:0006497 | ISS:UniProtKB | P | protein lipidation |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0006665 | IDA:UniProtKB | P | sphingolipid metabolic process |
KEGG Pathway Links
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_147851 | Arachidonic acid metabolism |
| REACT_150201 | Synthesis of 12-eicosatetraenoic acid derivatives |
Domain Information
UniProt Annotations
Entry Information
Gene Name
arachidonate 12-lipoxygenase, 12R type
Protein Entry
LX12B_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12R)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate. |
| Cofactor | Name=Fe cation; Xref=ChEBI |
| Disease | Ichthyosis, congenital, autosomal recessive 2 (ARCI2) [MIM |
| Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins. {ECO |
| Interaction | Q9BYJ1:ALOXE3; NbExp=2; IntAct=EBI-6925925, EBI-6925949; |
| Pathway | Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis. |
| Pathway | Lipid metabolism; sphingolipid metabolism. |
| Similarity | Belongs to the lipoxygenase family. |
| Similarity | Contains 1 PLAT domain. {ECO |
| Similarity | Contains 1 lipoxygenase domain. {ECO |
| Subcellular Location | Cytoplasm {ECO |
| Tissue Specificity | Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue. |
| Web Resource | Name=Protein Spotlight; Note=about water - Issue 153 of September 2013; URL="http://web.expasy.org/spotlight/back_issues/153/"; |
Identical and Related Proteins
Unique RefSeq proteins for LMP002433 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 4502053 | RefSeq | NP_001130 | 701 | arachidonate 12-lipoxygenase, 12R-type |
Identical Sequences to LMP002433 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:4502053 | DBBJ | BAJ21000.1 | 701 | arachidonate 12-lipoxygenase, 12R type, partial [synthetic construct] |
| GI:4502053 | GenBank | AAQ32814.1 | 701 | Sequence 2 from patent US 6569644 |
| GI:4502053 | GenBank | EAW90095.1 | 701 | arachidonate 12-lipoxygenase, 12R type [Homo sapiens] |
| GI:4502053 | GenBank | ACM80528.1 | 701 | Sequence 6026 from patent US 6812339 |
| GI:4502053 | GenBank | AHE01970.1 | 701 | Sequence 59819 from patent US 8586006 |
| GI:4502053 | GenBank | AIC48254.1 | 701 | ALOX12B, partial [synthetic construct] |
Related Sequences to LMP002433 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:4502053 | GenBank | EHH24497.1 | 701 | Arachidonate 12-lipoxygenase, 12R-type [Macaca mulatta] |
| GI:4502053 | GenBank | EHH57699.1 | 701 | Arachidonate 12-lipoxygenase, 12R-type [Macaca fascicularis] |
| GI:4502053 | RefSeq | XP_511864.2 | 701 | PREDICTED: arachidonate 12-lipoxygenase, 12R-type [Pan troglodytes] |
| GI:4502053 | RefSeq | XP_003818099.1 | 701 | PREDICTED: arachidonate 12-lipoxygenase, 12R-type [Pan paniscus] |
| GI:4502053 | RefSeq | XP_003912343.1 | 701 | PREDICTED: arachidonate 12-lipoxygenase, 12R-type [Papio anubis] |
| GI:4502053 | RefSeq | XP_004058584.1 | 701 | PREDICTED: arachidonate 12-lipoxygenase, 12R-type [Gorilla gorilla gorilla] |