Gene/Proteome Database (LMPD)
LMPD ID
LMP002507
Gene ID
Species
Homo sapiens (Human)
Gene Name
fatty acid synthase
Gene Symbol
Synonyms
FAS; OA-519; SDR27X1
Alternate Names
fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1
Chromosome
17
Map Location
17q25
EC Number
2.3.1.85
Summary
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| fatty acid synthase | |
|---|---|
| Refseq ID | NP_004095 |
| Protein GI | 41872631 |
| UniProt ID | P49327 |
| mRNA ID | NM_004104 |
| Length | 2511 |
| RefSeq Status | REVIEWED |
| MEEVVIAGMSGKLPESENLQEFWDNLIGGVDMVTDDDRRWKAGLYGLPRRSGKLKDLSRFDASFFGVHPKQAHTMDPQLRLLLEVTYEAIVDGGINPDSLRGTHTGVWVGVSGSETSEALSRDPETLVGYSMVGCQRAMMANRLSFFFDFRGPSIALDTACSSSLMALQNAYQAIHSGQCPAAIVGGINVLLKPNTSVQFLRLGMLSPEGTCKAFDTAGNGYCRSEGVVAVLLTKKSLARRVYATILNAGTNTDGFKEQGVTFPSGDIQEQLIRSLYQSAGVAPESFEYIEAHGTGTKVGDPQELNGITRALCATRQEPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGLWAPNLHFHSPNPEIPALLDGRLQVVDQPLPVRGGNVGINSFGFGGSNVHIILRPNTQPPPAPAPHATLPRLLRASGRTPEAVQKLLEQGLRHSQDLAFLSMLNDIAAVPATAMPFRGYAVLGGERGGPEVQQVPAGERPLWFICSGMGTQWRGMGLSLMRLDRFRDSILRSDEAVKPFGLKVSQLLLSTDESTFDDIVHSFVSLTAIQIGLIDLLSCMGLRPDGIVGHSLGEVACGYADGCLSQEEAVLAAYWRGQCIKEAHLPPGAMAAVGLSWEECKQRCPPGVVPACHNSKDTVTISGPQAPVFEFVEQLRKEGVFAKEVRTGGMAFHSYFMEAIAPPLLQELKKVIREPKPRSARWLSTSIPEAQWHSSLARTSSAEYNVNNLVSPVLFQEALWHVPEHAVVLEIAPHALLQAVLKRGLKPSCTIIPLMKKDHRDNLEFFLAGIGRLHLSGIDANPNALFPPVEFPAPRGTPLISPLIKWDHSLAWDVPAAEDFPNGSGSPSAAIYNIDTSSESPDHYLVDHTLDGRVLFPATGYLSIVWKTLARALGLGVEQLPVVFEDVVLHQATILPKTGTVSLEVRLLEASRAFEVSENGNLVVSGKVYQWDDPDPRLFDHPESPTPNPTEPLFLAQAEVYKELRLRGYDYGPHFQGILEASLEGDSGRLLWKDNWVSFMDTMLQMSILGSAKHGLYLPTRVTAIHIDPATHRQKLYTLQDKAQVADVVVSRWLRVTVAGGVHISGLHTESAPRRQQEQQVPILEKFCFTPHTEEGCLSERAALQEELQLCKGLVQALQTKVTQQGLKMVVPGLDGAQIPRDPSQQELPRLLSAACRLQLNGNLQLELAQVLAQERPKLPEDPLLSGLLDSPALKACLDTAVENMPSLKMKVVEVLAGHGHLYSRIPGLLSPHPLLQLSYTATDRHPQALEAAQAELQQHDVAQGQWDPADPAPSALGSADLLVCNCAVAALGDPASALSNMVAALREGGFLLLHTLLRGHPLGDIVAFLTSTEPQYGQGILSQDAWESLFSRVSLRLVGLKKSFYGSTLFLCRRPTPQDSPIFLPVDDTSFRWVESLKGILADEDSSRPVWLKAINCATSGVVGLVNCLRREPGGNRLRCVLLSNLSSTSHVPEVDPGSAELQKVLQGDLVMNVYRDGAWGAFRHFLLEEDKPEEPTAHAFVSTLTRGDLSSIRWVCSSLRHAQPTCPGAQLCTVYYASLNFRDIMLATGKLSPDAIPGKWTSQDSLLGMEFSGRDASGKRVMGLVPAKGLATSVLLSPDFLWDVPSNWTLEEAASVPVVYSTAYYALVVRGRVRPGETLLIHSGSGGVGQAAIAIALSLGCRVFTTVGSAEKRAYLQARFPQLDSTSFANSRDTSFEQHVLWHTGGKGVDLVLNSLAEEKLQASVRCLATHGRFLEIGKFDLSQNHPLGMAIFLKNVTFHGVLLDAFFNESSADWREVWALVQAGIRDGVVRPLKCTVFHGAQVEDAFRYMAQGKHIGKVVVQVLAEEPEAVLKGAKPKLMSAISKTFCPAHKSYIIAGGLGGFGLELAQWLIQRGVQKLVLTSRSGIRTGYQAKQVRRWRRQGVQVQVSTSNISSLEGARGLIAEAAQLGPVGGVFNLAVVLRDGLLENQTPEFFQDVCKPKYSGTLNLDRVTREACPELDYFVVFSSVSCGRGNAGQSNYGFANSAMERICEKRRHEGLPGLAVQWGAIGDVGILVETMSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:HPA | C | cytoplasm |
| GO:0005829 | TAS:Reactome | C | cytosol |
| GO:0070062 | IDA:UniProtKB | C | extracellular vesicular exosome |
| GO:0042587 | IEA:Ensembl | C | glycogen granule |
| GO:0005794 | IDA:HPA | C | Golgi apparatus |
| GO:0016020 | IDA:UniProtKB | C | membrane |
| GO:0005739 | IEA:Ensembl | C | mitochondrion |
| GO:0005886 | IDA:HPA | C | plasma membrane |
| GO:0047451 | IEA:UniProtKB-EC | F | 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity |
| GO:0004317 | IEA:InterPro | F | 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity |
| GO:0004316 | IEA:UniProtKB-EC | F | 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity |
| GO:0004315 | IEA:UniProtKB-EC | F | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| GO:0004313 | IEA:UniProtKB-EC | F | [acyl-carrier-protein] S-acetyltransferase activity |
| GO:0004314 | IEA:UniProtKB-EC | F | [acyl-carrier-protein] S-malonyltransferase activity |
| GO:0008144 | IEA:Ensembl | F | drug binding |
| GO:0047117 | IEA:UniProtKB-EC | F | enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity |
| GO:0004319 | IEA:InterPro | F | enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity |
| GO:0004312 | TAS:Reactome | F | fatty acid synthase activity |
| GO:0016295 | IEA:UniProtKB-EC | F | myristoyl-[acyl-carrier-protein] hydrolase activity |
| GO:0070402 | IEA:Ensembl | F | NADPH binding |
| GO:0004320 | IEA:UniProtKB-EC | F | oleoyl-[acyl-carrier-protein] hydrolase activity |
| GO:0016296 | IEA:UniProtKB-EC | F | palmitoyl-[acyl-carrier-protein] hydrolase activity |
| GO:0044822 | IDA:UniProtKB | F | poly(A) RNA binding |
| GO:0008270 | IEA:InterPro | F | zinc ion binding |
| GO:0006084 | IEA:Ensembl | P | acetyl-CoA metabolic process |
| GO:0044255 | TAS:Reactome | P | cellular lipid metabolic process |
| GO:0071353 | IEA:Ensembl | P | cellular response to interleukin-4 |
| GO:0006112 | TAS:Reactome | P | energy reserve metabolic process |
| GO:0006633 | IEA:UniProtKB-KW | P | fatty acid biosynthetic process |
| GO:0006631 | TAS:ProtInc | P | fatty acid metabolic process |
| GO:0035338 | TAS:Reactome | P | long-chain fatty-acyl-CoA biosynthetic process |
| GO:0001649 | IDA:UniProt | P | osteoblast differentiation |
| GO:0015939 | TAS:Reactome | P | pantothenate metabolic process |
| GO:0031325 | TAS:Reactome | P | positive regulation of cellular metabolic process |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0019432 | TAS:Reactome | P | triglyceride biosynthetic process |
| GO:0006766 | TAS:Reactome | P | vitamin metabolic process |
| GO:0006767 | TAS:Reactome | P | water-soluble vitamin metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa04152 | AMPK signaling pathway |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR009081 | Acyl carrier protein-like |
| IPR014043 | Acyl transferase |
| IPR016035 | Acyl transferase/acyl hydrolase/lysophospholipase |
| IPR001227 | Acyl transferase domain |
| IPR013149 | Alcohol dehydrogenase, C-terminal |
| IPR029058 | Alpha/Beta hydrolase fold |
| IPR018201 | Beta-ketoacyl synthase, active site |
| IPR014031 | Beta-ketoacyl synthase, C-terminal |
| IPR014030 | Beta-ketoacyl synthase, N-terminal |
| IPR023102 | Fatty acid synthase, domain 2 |
| IPR011032 | GroES (chaperonin 10)-like |
| IPR016036 | Malonyl-CoA ACP transacylase, ACP-binding |
| IPR013217 | Methyltransferase type 12 |
| IPR016040 | NAD(P)-binding domain |
| IPR006162 | Phosphopantetheine attachment site |
| IPR020843 | Polyketide synthase, enoylreductase domain |
| IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| IPR002198 | Short-chain dehydrogenase/reductase SDR |
| IPR001031 | Thioesterase |
| IPR016039 | Thiolase-like |
| IPR016038 | Thiolase-like, subgroup |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH. |
| Catalytic Activity | A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O. |
| Catalytic Activity | Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]. |
| Catalytic Activity | Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+). |
| Catalytic Activity | Acyl-[acyl-carrier-protein] + malonyl-[acyl- carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]. |
| Catalytic Activity | An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH. |
| Catalytic Activity | Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]. |
| Catalytic Activity | Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl- carrier-protein] + oleate. |
| Function | Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. |
| Interaction | Q99IB8:- (xeno); NbExp=6; IntAct=EBI-356658, EBI-6927928; |
| Miscellaneous | The relatively low beta-ketoacyl synthase activity may be attributable to the low 4'-phosphopantetheine content of the protein. |
| Sequence Caution | Sequence=AAB35516.1; Type=Erroneous initiation; Evidence= ; Sequence=AAC50259.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors.; Evidence= ; Sequence=BAE06070.1; Type=Erroneous initiation; Evidence= ; |
| Similarity | Contains 1 acyl carrier domain. {ECO |
| Subcellular Location | Cytoplasm . Melanosome . Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Subunit | Homodimer which is arranged in a head to tail fashion. {ECO |
| Tissue Specificity | Ubiquitous. Prominent expression in brain, lung, and liver. {ECO |
Identical and Related Proteins
Unique RefSeq proteins for LMP002507 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 41872631 | RefSeq | NP_004095 | 2511 | fatty acid synthase |
Identical Sequences to LMP002507 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:41872631 | EMBL | CEF39429.1 | 2511 | unnamed protein product [Homo sapiens] |
| GI:41872631 | GenBank | ADT48731.1 | 2511 | Sequence 748 from patent US 7842467 |
| GI:41872631 | GenBank | AEK13652.1 | 2511 | Sequence 64 from patent US 7972785 |
| GI:41872631 | GenBank | AEN35196.1 | 2511 | Sequence 1124 from patent US 7998689 |
| GI:41872631 | GenBank | AGN43306.1 | 2511 | Sequence 1 from patent US 8450350 |
| GI:41872631 | GenBank | AGY17321.1 | 2511 | Sequence 1 from patent US 8546432 |
Related Sequences to LMP002507 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:41872631 | DBBJ | BAE06070.1 | 2548 | FASN variant protein, partial [Homo sapiens] |
| GI:41872631 | GenBank | ACR85656.1 | 2511 | Sequence 215 from patent US 7521195 |
| GI:41872631 | GenBank | ADA19299.1 | 2511 | Sequence 1123 from patent US 7608413 |
| GI:41872631 | GenBank | ADT46363.1 | 2511 | Sequence 24 from patent US 7842466 |
| GI:41872631 | GenBank | ADT48730.1 | 2511 | Sequence 747 from patent US 7842467 |
| GI:41872631 | GenBank | AEN35195.1 | 2511 | Sequence 1123 from patent US 7998689 |