Gene/Proteome Database (LMPD)

LMPD ID
LMP002535
Gene ID
242291
Species
Mus musculus (Mouse)
Gene Name
inositol monophosphatase domain containing 1
Gene Symbol
Impad1
Synonyms
1110001C20Rik; AA408880; AI451589; AL022796; B230207P20; Jaws; gPAPP
Alternate Names
inositol monophosphatase 3; IMP 3; IMPase 3; golgi-resident PAP phosphatase; myo-inositol monophosphatase A3; inositol-1(or 4)-monophosphatase 3; inositol monophosphatase domain-containing protein 1; Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase
Chromosome
4
Map Location
4 A1|4
EC Number
3.1.3.25

Proteins

inositol monophosphatase 3
Refseq ID NP_808398
Protein GI 29244210
UniProt ID Q80V26
mRNA ID NM_177730
Length 356
RefSeq Status PROVISIONAL
MAPMGIRLSPLGVAVFFLLGLGVLYHLYSGFLAGRFSLFGLGSEPAAGEAEVASDGGTVDLREMLAVAVLAAERGGDEVRRVRESNVLHEKSKGKTREGADDKMTSGDVLSNRKMFYLLKTAFPNVQINTEEHVDASDKEVIVWNRKIPEDILKEIAAPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPVLGVIHKPFSEYTAWAMVDGGSNVKARSSYNEKTPKIIVSRSHAGMVKQVALQTFGNQTSIIPAGGAGYKVLALLDVPDMTQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLNGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKSGH

Gene Information

Entrez Gene ID
242291
Gene Name
inositol monophosphatase domain containing 1
Gene Symbol
Impad1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 ISO:MGI C Golgi apparatus
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0008441 IEA:UniProtKB-EC F 3'(2'),5'-bisphosphate nucleotidase activity
GO:0008254 IDA:MGI F 3'-nucleotidase activity
GO:0008934 IEA:UniProtKB-EC F inositol monophosphate 1-phosphatase activity
GO:0052832 IEA:UniProtKB-EC F inositol monophosphate 3-phosphatase activity
GO:0052833 IEA:UniProtKB-EC F inositol monophosphate 4-phosphatase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0002063 IMP:MGI P chondrocyte development
GO:0030204 IMP:MGI P chondroitin sulfate metabolic process
GO:0016311 IDA:GOC P dephosphorylation
GO:0042733 IMP:MGI P embryonic digit morphogenesis
GO:0001958 IMP:MGI P endochondral ossification
GO:0006021 IEA:UniProtKB-UniPathway P inositol biosynthetic process
GO:0046854 IEA:InterPro P phosphatidylinositol phosphorylation
GO:0009791 IMP:MGI P post-embryonic development
GO:0001501 IMP:MGI P skeletal system development

KEGG Pathway Links

KEGG Pathway ID Description
mmu00562 Inositol phosphate metabolism
mmu01100 Metabolic pathways
mmu00920 Sulfur metabolism

Domain Information

InterPro Annotations

Accession Description
IPR000760 Inositol_monophosphatase
IPR020550 Inositol monophosphatase, conserved site

UniProt Annotations

Entry Information

Gene Name
inositol monophosphatase domain containing 1
Protein Entry
IMPA3_MOUSE
UniProt ID
Q80V26
Species
Mouse

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=21 uM for 3'-phosphoadenosine 5'- phosphate (PAP) {ECO:0000269|PubMed:18695242}; Vmax=9.5 umol/min/mg enzyme {ECO:0000269|PubMed:18695242};
Catalytic Activity Adenosine 3',5'-bisphosphate + H(2)O = adenosine 5'-phosphate + phosphate. {ECO:0000269|PubMed:18695242}.
Catalytic Activity Myo-inositol phosphate + H(2)O = myo-inositol + phosphate. {ECO:0000269|PubMed:18695242}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Developmental Stage At 18.5 dpc, widely expressed with enhanced levels in brain, spinal cord, lung and kidney, including medulla and cortex. In the developing brain, strongly expressed in the neopallial cortex and throughout the cerebellum with intense expression within the developing Purkinje cells and adjacent choroid plexus. Strong expression also observed within the pons and throughout the medulla oblongata. In the lung, expressed in individual pneumocytes and particularly in cells surrounding developing bronchi/bronchioles. Moderate expression in chondrocytes of costal cartilage and in the surrounding perichondrium. {ECO:0000269|PubMed:18695242}.
Disruption Phenotype Mutant animals experience severe respiratory distress and died within minutes after birth. At 18.5 dpc, lungs exhibit small alveolar spaces and thickened septa. The rib cage cartilage is hypocellular with abnormal, fibrous-appearing extracellular matrix. Animals show severe skeletal abnormalities, most notably in the longitudinal growth of bones formed by endochondral ossification. The length of the axial skeleton is reduced. The appendicular bones of the upper limbs, as well as the ilium, femur, tibia and fibula of the lower limbs are markedly shorter than in wild-type littermates. The rib cages display malformation characterized by reduced sternal length and correspondingly diminished rib spacing. The process of intramembranous ossification is normal. Mutant animals exhibit a deficiency in glycosaminoglycan sulfation. Mutant cartilage and lung exhibit a substantial decrease in chondroitin 4-sulfate and an increase in nonsulfated chondroitin compared with wild type tissue. {ECO:0000269|PubMed:18695242}.
Enzyme Regulation Strongly inhibited by lithium. {ECO:0000269|PubMed:18695242}.
Function May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. {ECO:0000269|PubMed:18695242}.
Pathway Polyol metabolism; myo-inositol biosynthesis; myo- inositol from D-glucose 6-phosphate: step 2/2.
Ptm Contains N-linked glycan resistant to endoglycosydase H. {ECO:0000250}.
Similarity Belongs to the inositol monophosphatase family. {ECO:0000305}.
Subcellular Location Golgi apparatus {ECO:0000269|PubMed:18695242}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP002535 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
29244210 RefSeq NP_808398 356 inositol monophosphatase 3

Identical Sequences to LMP002535 proteins

Reference Database Accession Length Protein Name
GI:29244210 GenBank AAH48776.1 356 Inositol monophosphatase domain containing 1 [Mus musculus]
GI:29244210 GenBank EDL05698.1 356 inositol monophosphatase domain containing 1 [Mus musculus]
GI:29244210 GenBank AAI45953.1 356 Inositol monophosphatase domain containing 1 [Mus musculus]
GI:29244210 GenBank AAI38210.1 356 Inositol monophosphatase domain containing 1 [Mus musculus]
GI:29244210 SwissProt Q80V26.1 356 RecName: Full=Inositol monophosphatase 3; Short=IMP 3; Short=IMPase 3; AltName: Full=Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase; Short=Golgi-resident PAP phosphatase; Short=gPAPP; AltName: Full=Inositol monophosphatase domain-containing protein 1; AltName: Full=Inositol-1(or 4)-monophosphatase 3; AltName: Full=Myo-inositol monophosphatase A3 [Mus musculus]

Related Sequences to LMP002535 proteins

Reference Database Accession Length Protein Name
GI:29244210 GenBank EDM11627.1 356 inositol monophosphatase domain containing 1 [Rattus norvegicus]
GI:29244210 RefSeq NP_001008772.2 356 inositol monophosphatase 3 [Rattus norvegicus]
GI:29244210 RefSeq XP_005362339.1 359 PREDICTED: inositol monophosphatase 3 [Microtus ochrogaster]
GI:29244210 RefSeq XP_008774114.1 356 PREDICTED: inositol monophosphatase 3 [Rattus norvegicus]
GI:29244210 RefSeq XP_008761786.1 356 PREDICTED: inositol monophosphatase 3 [Rattus norvegicus]
GI:29244210 SwissProt D4AD37.1 356 RecName: Full=Inositol monophosphatase 3; Short=IMP 3; Short=IMPase 3; AltName: Full=Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase; Short=Golgi-resident PAP phosphatase; Short=gPAPP; AltName: Full=Inositol monophosphatase domain-containing protein 1; AltName: Full=Inositol-1(or 4)-monophosphatase 3; AltName: Full=Myo-inositol monophosphatase A3 [Rattus norvegicus]