Gene/Proteome Database (LMPD)

LMPD ID
LMP002703
Gene ID
10400
Species
Homo sapiens (Human)
Gene Name
phosphatidylethanolamine N-methyltransferase
Gene Symbol
PEMT
Synonyms
PEAMT; PEMPT; PEMT2; PNMT
Alternate Names
phosphatidylethanolamine N-methyltransferase
Chromosome
17
Map Location
17p11.2
EC Number
2.1.1.17
Summary
Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]
Orthologs
View orthologs and multiple alignments for PEMT

Proteins

phosphatidylethanolamine N-methyltransferase isoform 1
Refseq ID NP_680477
Protein GI 22538478
UniProt ID Q9UBM1
mRNA ID NM_148172
Length 236
RefSeq Status REVIEWED
MKRSGNPGAEVTNSSVAGPDCCGGLGNIDFRQADFCVMTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS
phosphatidylethanolamine N-methyltransferase isoform 2
Refseq ID NP_680478
Protein GI 22538480
UniProt ID Q9UBM1
mRNA ID NM_148173
Length 199
RefSeq Status REVIEWED
MTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS
phosphatidylethanolamine N-methyltransferase isoform 2
Refseq ID NP_009100
Protein GI 22538482
UniProt ID Q9UBM1
mRNA ID NM_007169
Length 199
RefSeq Status REVIEWED
Protein sequence is identical to GI:22538480 (mRNA isoform)
phosphatidylethanolamine N-methyltransferase isoform 3
Refseq ID NP_001254481
Protein GI 388890237
UniProt ID Q9UBM1
mRNA ID NM_001267552
Length 232
RefSeq Status REVIEWED
MKRSGNPGAEVTNSSVAGPDCCGGLGNIDFRQADFCVMTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIIPAPAGAVGSEARQPHGPAPDGAGGPHLHSGSPIRRALHR
phosphatidylethanolamine N-methyltransferase isoform 4
Refseq ID NP_001254480
Protein GI 388890235
UniProt ID Q9UBM1
mRNA ID NM_001267551
Length 214
RefSeq Status REVIEWED
MKRSGNPGAEADFCVMTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS

Gene Information

Entrez Gene ID
10400
Gene Name
phosphatidylethanolamine N-methyltransferase
Gene Symbol
PEMT
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005789 TAS:Reactome C endoplasmic reticulum membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005739 IEA:UniProtKB-KW C mitochondrion
GO:0080101 IEA:UniProtKB-EC F phosphatidyl-N-dimethylethanolamine N-methyltransferase activity
GO:0000773 IEA:UniProtKB-EC F phosphatidyl-N-methylethanolamine N-methyltransferase activity
GO:0004608 TAS:ProtInc F phosphatidylethanolamine N-methyltransferase activity
GO:0008283 TAS:ProtInc P cell proliferation
GO:0046474 TAS:Reactome P glycerophospholipid biosynthetic process
GO:0006629 TAS:ProtInc P lipid metabolic process
GO:0006656 TAS:Reactome P phosphatidylcholine biosynthetic process
GO:0006644 TAS:Reactome P phospholipid metabolic process
GO:0044281 TAS:Reactome P small molecule metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
hsa00564 Glycerophospholipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_121238 Synthesis of PC

Domain Information

InterPro Annotations

Accession Description
IPR024960 Phosphatidyl-N-methylethanolamine/N-methyltransferase
IPR007318 Phospholipid methyltransferase

UniProt Annotations

Entry Information

Gene Name
phosphatidylethanolamine N-methyltransferase
Protein Entry
PEMT_HUMAN
UniProt ID
Q9UBM1
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q9UBM1-1; Sequence=Displayed; Name=2; IsoId=Q9UBM1-2; Sequence=VSP_037311; Note=Variant in position: 11:V->A.; Name=3; IsoId=Q9UBM1-3; Sequence=VSP_037311, VSP_046034; Note=No experimental confirmation available. Ref.5 (BAG63603) sequence is in conflict in position: 222:S->N. ;
Catalytic Activity S-adenosyl-L-methionine + phosphatidyl-N- dimethylethanolamine = S-adenosyl-L-homocysteine + phosphatidylcholine.
Catalytic Activity S-adenosyl-L-methionine + phosphatidyl-N- methylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N- dimethylethanolamine.
Catalytic Activity S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine. {ECO
Enzyme Regulation The first methylation is rate-limiting.
Function Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC).
Pathway Phospholipid metabolism; phosphatidylcholine biosynthesis.
Sequence Caution Sequence=AAF14867.1; Type=Erroneous initiation; Evidence= ;
Similarity Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.
Subcellular Location Endoplasmic reticulum membrane {ECO
Web Resource Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/pemt/";

Identical and Related Proteins

Unique RefSeq proteins for LMP002703 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
22538478 RefSeq NP_680477 236 phosphatidylethanolamine N-methyltransferase isoform 1
22538480 RefSeq NP_680478 199 phosphatidylethanolamine N-methyltransferase isoform 2
388890237 RefSeq NP_001254481 232 phosphatidylethanolamine N-methyltransferase isoform 3
388890235 RefSeq NP_001254480 214 phosphatidylethanolamine N-methyltransferase isoform 4

Identical Sequences to LMP002703 proteins

Reference Database Accession Length Protein Name
GI:22538478 GenBank AAH50593.1 236 Phosphatidylethanolamine N-methyltransferase [Homo sapiens]
GI:22538478 GenBank ACB21050.1 236 phosphatidylethanolamine N-methyltransferase [Homo sapiens]
GI:22538478 GenBank ADQ33077.1 236 phosphatidylethanolamine N-methyltransferase, partial [synthetic construct]
GI:22538480 GenBank AFO05892.1 199 Sequence 530 from patent US 8216786
GI:22538478 GenBank AFO05893.1 236 Sequence 531 from patent US 8216786
GI:22538480 GenBank AFO05894.1 199 Sequence 532 from patent US 8216786
GI:22538480 GenBank AHD71803.1 199 Sequence 6497 from patent US 8586006
GI:22538478 GenBank AHD71804.1 236 Sequence 6498 from patent US 8586006
GI:22538480 GenBank AHD71805.1 199 Sequence 6499 from patent US 8586006
GI:22538478 GenBank AIC55768.1 236 PEMT, partial [synthetic construct]
GI:22538480 RefSeq NP_009100.2 199 phosphatidylethanolamine N-methyltransferase isoform 2 [Homo sapiens]
GI:22538480 SwissProt Q9UBM1.4 199 RecName: Full=Phosphatidylethanolamine N-methyltransferase; Short=PEAMT; Short=PEMT; AltName: Full=PEMT2 [Homo sapiens]

Related Sequences to LMP002703 proteins

Reference Database Accession Length Protein Name
GI:388890237 DBBJ BAG63603.1 232 unnamed protein product [Homo sapiens]
GI:22538478 EMBL CAI93558.1 236 unnamed protein product [Homo sapiens]
GI:22538480 GenBank AAF14867.1 236 phosphotidylethanolamine N-methyltransferase [Homo sapiens]
GI:388890237 GenBank AAF14867.1 236 phosphotidylethanolamine N-methyltransferase [Homo sapiens]
GI:388890235 GenBank AAF14867.1 236 phosphotidylethanolamine N-methyltransferase [Homo sapiens]
GI:22538478 GenBank AAH00557.1 236 Phosphatidylethanolamine N-methyltransferase [Homo sapiens]
GI:388890235 GenBank AAH50593.1 236 Phosphatidylethanolamine N-methyltransferase [Homo sapiens]
GI:22538480 GenBank AAH50593.1 236 Phosphatidylethanolamine N-methyltransferase [Homo sapiens]
GI:22538478 GenBank ABH92438.1 236 Sequence 409 from patent US 7060479
GI:22538478 GenBank EAW55700.1 236 phosphatidylethanolamine N-methyltransferase, isoform CRA_a [Homo sapiens]
GI:22538478 GenBank ABW56377.1 236 Sequence 409 from patent US 7271243
GI:388890235 GenBank ACB21050.1 236 phosphatidylethanolamine N-methyltransferase [Homo sapiens]
GI:22538480 GenBank ACB21050.1 236 phosphatidylethanolamine N-methyltransferase [Homo sapiens]
GI:388890235 GenBank AFO05893.1 236 Sequence 531 from patent US 8216786
GI:22538480 GenBank AFO05893.1 236 Sequence 531 from patent US 8216786
GI:388890235 GenBank AHD71804.1 236 Sequence 6498 from patent US 8586006
GI:22538480 GenBank AHD71804.1 236 Sequence 6498 from patent US 8586006
GI:388890237 GenBank AHD71804.1 236 Sequence 6498 from patent US 8586006
GI:22538478 GenBank AIC55767.1 236 PEMT, partial [synthetic construct]
GI:388890235 GenBank AIC55768.1 236 PEMT, partial [synthetic construct]
GI:22538480 GenBank AIC55768.1 236 PEMT, partial [synthetic construct]
GI:388890237 RefSeq XP_008008726.1 226 PREDICTED: phosphatidylethanolamine N-methyltransferase isoform X6 [Chlorocebus sabaeus]
GI:388890237 RefSeq XP_009188057.1 226 PREDICTED: phosphatidylethanolamine N-methyltransferase isoform X3 [Papio anubis]
GI:388890237 RefSeq XP_010367177.1 226 PREDICTED: phosphatidylethanolamine N-methyltransferase isoform X3 [Rhinopithecus roxellana]