Gene/Proteome Database (LMPD)
LMPD ID
LMP002710
Gene ID
Species
Mus musculus (Mouse)
Gene Name
lecithin cholesterol acyltransferase
Gene Symbol
Synonyms
AI046659; D8Wsu61e
Alternate Names
phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase; lecithin-cholesterol acyltransferase Lcat
Chromosome
8
Map Location
8 D3|8 53.06 cM
EC Number
2.3.1.43
Proteins
| phosphatidylcholine-sterol acyltransferase precursor | |
|---|---|
| Refseq ID | NP_032516 |
| Protein GI | 244791354 |
| UniProt ID | P16301 |
| mRNA ID | NM_008490 |
| Length | 438 |
| RefSeq Status | VALIDATED |
| MGLPGSPWQRVLLLLGLLLPPATPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNRLEAKLDKPDVVNWMCYRKTEDFFTIWLDFNLFLPLGVDCWIDNTRIVYNHSSGRVSNAPGVQIRVPGFGKTESVEYVDDNKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLAPHQQDEYYKKLAGLVEEMYAAYGKPVFLIGHSLGCLHVLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKAMRILASGDNQGIPILSNIKLKEEQRITTTSPWMLPAPHVWPEDHVFISTPNFNYTVQDFERFFTDLHFEEGWHMFLQSRDLLERLPAPGVEVYCLYGVGRPTPHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQPVHLLPMNETDHLNMVFSNKTLEHINAILLGAYRTPKSPAASPSPPPPE | |
| sig_peptide: 1..24 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2540 peptide sequence: MGLPGSPWQRVLLLLGLLLPPATP mat_peptide: 25..438 product: Phosphatidylcholine-sterol acyltransferase experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P16301.2) calculated_mol_wt: 47225 peptide sequence: FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNRLEAKLDKPDVVNWMCYRKTEDFFTIWLDFNLFLPLGVDCWIDNTRIVYNHSSGRVSNAPGVQIRVPGFGKTESVEYVDDNKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLAPHQQDEYYKKLAGLVEEMYAAYGKPVFLIGHSLGCLHVLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKAMRILASGDNQGIPILSNIKLKEEQRITTTSPWMLPAPHVWPEDHVFISTPNFNYTVQDFERFFTDLHFEEGWHMFLQSRDLLERLPAPGVEVYCLYGVGRPTPHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQPVHLLPMNETDHLNMVFSNKTLEHINAILLGAYRTPKSPAASPSPPPPE | |
Gene Information
Entrez Gene ID
Gene Name
lecithin cholesterol acyltransferase
Gene Symbol
Species
Mus musculus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005615 | IDA:MGI | C | extracellular space |
| GO:0070062 | IEA:Ensembl | C | extracellular vesicular exosome |
| GO:0034364 | IEA:Ensembl | C | high-density lipoprotein particle |
| GO:0004607 | IDA:MGI | F | phosphatidylcholine-sterol O-acyltransferase activity |
| GO:0034435 | IEA:Ensembl | P | cholesterol esterification |
| GO:0042632 | IEA:Ensembl | P | cholesterol homeostasis |
| GO:0008203 | IDA:MGI | P | cholesterol metabolic process |
| GO:0030301 | ISO:MGI | P | cholesterol transport |
| GO:0034375 | IEA:Ensembl | P | high-density lipoprotein particle remodeling |
| GO:0042158 | IDA:MGI | P | lipoprotein biosynthetic process |
| GO:0006656 | IEA:Ensembl | P | phosphatidylcholine biosynthetic process |
| GO:0090107 | IDA:UniProtKB | P | regulation of high-density lipoprotein particle assembly |
| GO:0043691 | IEA:Ensembl | P | reverse cholesterol transport |
| GO:0034372 | IEA:Ensembl | P | very-low-density lipoprotein particle remodeling |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| mmu00564 | Glycerophospholipid metabolism |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Gene Name
lecithin cholesterol acyltransferase
Protein Entry
LCAT_MOUSE
UniProt ID
Species
Mouse
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Phosphatidylcholine + a sterol = 1- acylglycerophosphocholine + a sterol ester. {ECO:0000255|PROSITE- ProRule:PRU10037}. |
| Developmental Stage | In the testis, expressed days 4,8, 14, and 35 of postnatal life with highest levels at day 35. In the brain, expressed in fetal stages and levels begin to rise after day 4 after birth and continue to increase through suckling and weaning reaching a peak at postnatal day 24. In the liver, expressed in fetal life from day 16-21 of gestation with a 3-fold increase in the four final days of gestation. {ECO:0000269|PubMed:2600083}. |
| Disruption Phenotype | Null mice exhibit a 7-fold increase in the cholesteryl ester fatty acid CEFA ratio of APOB lipoprotein CEs. There is also a 3.6 increase in vascular ring O(2) production and plasma phospholipid (PL)-bound-F2-isoprostane levels. This effect is paradoxically reversed in the APOE knockout background. {ECO:0000269|PubMed:11809774, ECO:0000269|PubMed:11893779, ECO:0000269|PubMed:19065001}. |
| Enzyme Regulation | APOA1 is the most potent activator in plasma. Also activated by APOE, APOC1 and APOA4. {ECO:0000269|PubMed:15654758, ECO:0000269|PubMed:19065001}. |
| Function | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (By similarity). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. {ECO:0000250, ECO:0000269|PubMed:11809774, ECO:0000269|PubMed:11893779, ECO:0000269|PubMed:15654758, ECO:0000269|PubMed:19065001}. |
| Similarity | Belongs to the AB hydrolase superfamily. Lipase family. {ECO:0000305}. |
| Subcellular Location | Secreted. Note=Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid (CSF) (By similarity). {ECO:0000250}. |
| Tissue Specificity | Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neurons and neuroglia. {ECO:0000269|PubMed:19065001, ECO:0000269|PubMed:2600083}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP002710 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 244791354 | RefSeq | NP_032516 | 438 | phosphatidylcholine-sterol acyltransferase precursor |
Identical Sequences to LMP002710 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:244791354 | DBBJ | BAE28903.1 | 438 | unnamed protein product [Mus musculus] |
| GI:244791354 | GenBank | AAH28861.1 | 438 | Lecithin cholesterol acyltransferase [Mus musculus] |
| GI:244791354 | SwissProt | P16301.2 | 438 | RecName: Full=Phosphatidylcholine-sterol acyltransferase; AltName: Full=Lecithin-cholesterol acyltransferase; AltName: Full=Phospholipid-cholesterol acyltransferase; Flags: Precursor [Mus musculus] |
Related Sequences to LMP002710 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:244791354 | DBBJ | BAB23665.1 | 444 | unnamed protein product, partial [Mus musculus] |
| GI:244791354 | GenBank | AAA39419.1 | 438 | cholesterol acyltransferase [Mus musculus] |
| GI:244791354 | GenBank | AAL11035.1 | 438 | lecithin-cholesterol acyltransferase Lcat [Mus musculus] |
| GI:244791354 | GenBank | AAH91155.1 | 440 | Lecithin cholesterol acyltransferase [Rattus norvegicus] |
| GI:244791354 | GenBank | EDL92424.1 | 440 | lecithin cholesterol acyltransferase, isoform CRA_b [Rattus norvegicus] |
| GI:244791354 | GenBank | AGM78123.1 | 437 | Sequence 2 from patent US 8426358 |