Gene/Proteome Database (LMPD)
Proteins
| acetylcholinesterase precursor | |
|---|---|
| Refseq ID | NP_033729 |
| Protein GI | 13928664 |
| UniProt ID | P21836 |
| mRNA ID | NM_009599 |
| Length | 614 |
| RefSeq Status | VALIDATED |
| MRPPWYPLHTPSLAFPLLFLLLSLLGGGARAEGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL | |
| sig_peptide: 1..31 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 3406 peptide sequence: MRPPWYPLHTPSLAFPLLFLLLSLLGGGARA mat_peptide: 32..614 product: Acetylcholinesterase experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P21836.1) calculated_mol_wt: 64781 peptide sequence: EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL sig_peptide: 1..31 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 3406 peptide sequence: MRPPWYPLHTPSLAFPLLFLLLSLLGGGARA mat_peptide: 32..614 product: Acetylcholinesterase experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P21836.1) calculated_mol_wt: 64781 peptide sequence: EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL | |
| acetylcholinesterase precursor | |
|---|---|
| Refseq ID | NP_001276939 |
| Protein GI | 586597902 |
| UniProt ID | P21836 |
| mRNA ID | NM_001290010 |
| Length | 614 |
| RefSeq Status | VALIDATED |
| Protein sequence is identical to GI:13928664 (mRNA isoform) | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005794 | IEA:Ensembl | C | Golgi apparatus |
| GO:0031225 | IEA:UniProtKB-KW | C | anchored component of membrane |
| GO:0030424 | IBA:RefGenome | C | axon |
| GO:0005605 | IDA:MGI | C | basal lamina |
| GO:0030054 | IEA:UniProtKB-KW | C | cell junction |
| GO:0009986 | IDA:MGI | C | cell surface |
| GO:0030425 | IBA:RefGenome | C | dendrite |
| GO:0005788 | IBA:RefGenome | C | endoplasmic reticulum lumen |
| GO:0005615 | IDA:MGI | C | extracellular space |
| GO:0031594 | IDA:MGI | C | neuromuscular junction |
| GO:0048471 | IEA:Ensembl | C | perinuclear region of cytoplasm |
| GO:0005886 | IDA:MGI | C | plasma membrane |
| GO:0045211 | IBA:RefGenome | C | postsynaptic membrane |
| GO:0042734 | IBA:RefGenome | C | presynaptic membrane |
| GO:0045202 | IDA:MGI | C | synapse |
| GO:0003990 | IDA:MGI | F | acetylcholinesterase activity |
| GO:0052689 | IBA:RefGenome | F | carboxylic ester hydrolase activity |
| GO:0004104 | IBA:RefGenome | F | cholinesterase activity |
| GO:0043236 | IPI:MGI | F | laminin binding |
| GO:0043621 | IPI:MGI | F | protein self-association |
| GO:0017171 | IEA:Ensembl | F | serine hydrolase activity |
| GO:0006581 | IDA:MGI | P | acetylcholine catabolic process |
| GO:0019695 | IBA:RefGenome | P | choline metabolic process |
| GO:0045212 | IMP:MGI | P | neurotransmitter receptor biosynthetic process |
| GO:0002076 | IEA:Ensembl | P | osteoblast development |
| GO:0051262 | IDA:MGI | P | protein tetramerization |
| GO:0031623 | IMP:MGI | P | receptor internalization |
| GO:0050770 | IBA:RefGenome | P | regulation of axonogenesis |
| GO:0048814 | IBA:RefGenome | P | regulation of dendrite morphogenesis |
| GO:0001919 | IMP:MGI | P | regulation of receptor recycling |
| GO:0060041 | IMP:MGI | P | retina development in camera-type eye |
| GO:0007416 | IBA:RefGenome | P | synapse assembly |
| GO:0007271 | IBA:RefGenome | P | synaptic transmission, cholinergic |
KEGG Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=T; IsoId=P21836-1; Sequence=Displayed; Name=H; IsoId=P21836-2; Sequence=Not described; Note=No experimental confirmation available.; |
| Catalytic Activity | Acetylcholine + H(2)O = choline + acetate. |
| Function | Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. |
| Miscellaneous | Synapses usually contain asymmetric molecules of cholinesterase, with a collagen-like part disulfide-bonded to the catalytic part. A different, globular type of cholinesterase occurs on the outer surfaces of cell membranes, including those of erythrocytes. |
| Miscellaneous | This is the catalytic subunit of an asymmetric or soluble form of ACHE. |
| Similarity | Belongs to the type-B carboxylesterase/lipase family. {ECO:0000305}. |
| Subcellular Location | Cell junction, synapse. Secreted. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. |
| Subcellular Location | Isoform H: Cell membrane; Lipid-anchor, GPI- anchor; Extracellular side. |
| Subunit | Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers (By similarity). Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers. {ECO:0000250, ECO:0000269|PubMed:11804574, ECO:0000269|PubMed:12505979}. |
| Tissue Specificity | Predominates in most expressing tissues except erythrocytes where a glycophospholipid-attached form of ACHE predominates. |
Identical and Related Proteins
Unique RefSeq proteins for LMP002757 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 13928664 | RefSeq | NP_033729 | 614 | acetylcholinesterase precursor |
Identical Sequences to LMP002757 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:13928664 | DBBJ | BAC31641.1 | 614 | unnamed protein product [Mus musculus] |
| GI:13928664 | DBBJ | BAC32595.1 | 614 | unnamed protein product [Mus musculus] |
| GI:13928664 | DBBJ | BAE24373.1 | 614 | unnamed protein product [Mus musculus] |
| GI:13928664 | GenBank | AAH46327.1 | 614 | Ache protein [Mus musculus] |
| GI:13928664 | GenBank | EDL19281.1 | 614 | acetylcholinesterase [Mus musculus] |
| GI:13928664 | RefSeq | NP_001276939.1 | 614 | acetylcholinesterase precursor [Mus musculus] |
Related Sequences to LMP002757 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:13928664 | EMBL | CAJ90606.1 | 614 | unnamed protein product [Mus musculus] |
| GI:13928664 | GenBank | AAB24586.1 | 614 | acetylcholinesterase T subunit [Rattus sp.] |
| GI:13928664 | GenBank | AAH94521.1 | 614 | Acetylcholinesterase [Rattus norvegicus] |
| GI:13928664 | GenBank | EDM13278.1 | 614 | acetylcholinesterase [Rattus norvegicus] |
| GI:13928664 | GenBank | ACF22905.1 | 614 | acetylcholinesterase [Mus musculus] |
| GI:13928664 | SwissProt | P37136.1 | 614 | RecName: Full=Acetylcholinesterase; Short=AChE; Flags: Precursor [Rattus norvegicus] |