Gene/Proteome Database (LMPD)

LMPD ID
LMP002880
Gene ID
74055
Species
Mus musculus (Mouse)
Gene Name
phospholipase C, epsilon 1
Gene Symbol
Plce1
Synonyms
4933403A21Rik; PLCepsilon; Plce; mKIAA1516
Alternate Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; PLC-epsilon-1; phospholipase C-epsilon-1; phosphoinositide phospholipase C-epsilon-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase epsilon-1
Chromosome
19
Map Location
19 D1|19
EC Number
3.1.4.11

Proteins

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Refseq ID NP_062534
Protein GI 134053943
UniProt ID Q8K4S1
mRNA ID NM_019588
Length 2282
RefSeq Status VALIDATED
MTSEEMAASVLIPVTQRKVASAQSVAEERSVKVSDAGIPRARAGRQGALIPPTISQWNKHKEESSRSDLSKVFSIARGELVCDENSNEEGWEENAPDSPENHAMNGNSLVQSHQHQFPRSQLCEARDSVTEDPCLQPGIPSPLERKVLPGIQLEMEDSPMDVSPAGSQPRIMESSGPHSDRNTAVFHFHYEADRTMSDAFHTLSENLILDDCANCVTLPGGQQNKNCMAYACKLVELTRTCGSKNGQVQCEHCTSLRDEYLCFESSCSKADEVCSGGGFCEDGFAHGPAAKTFLNPLEDFSDNCEDVDDFFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIMRTLPSGCIGPAAWNYVDQKKAGLLWPCGNVMGTLSAMDIRQSGSQRLSEAQWCLIYSAVRRGEEIEDTVGSLLHCSTQLPNSETAHGRIEDGPCLKQCVRDTECEFRATLQRTSIAQYITGSLLEATTSLGARSGLLSSFGGSTGRIMLKERQLGTSMANSNPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKASISESILTSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNSEKELTVNSIFQVIRSCSRSLEMEEEDSASEGSGSRKNSLKDKARWQFIIGDLLDSENDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARPKLGVLSNMAEPGKFPSPGNAGVSGLAEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAEMLFSGLLELTTAVRKIRRFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQDVNEQEESEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIRGGMQGFQSFMVSDSNMSFVEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSVSMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQAQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSSRGKERKSRKSIFGNNPGRMSPGETAPFNRTSGKGSCEGMRHTWEESSPLSPSTSLSAIIRTPKCYHISSLNENAAKRLCRRGSQKLIQHTAYQLLRTYPAATRIDSSNPNPIMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDNLDPAIYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLRALKRGYRHLQLRNLHNEILEISSLFINSRRMEENPSGSSMPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQVLAVDQDTKCTATDYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKSIVFDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVLKDAANKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQSRGLPSPPQLVASESVQSKEEKPVGALSSSDTVGYQQ

Gene Information

Entrez Gene ID
74055
Gene Name
phospholipase C, epsilon 1
Gene Symbol
Plce1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 ISS:UniProtKB C cytosol
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0005886 ISS:UniProtKB C plasma membrane
GO:0005509 IEA:InterPro F calcium ion binding
GO:0019899 ISS:UniProtKB F enzyme binding
GO:0005085 IEA:UniProtKB-KW F guanyl-nucleotide exchange factor activity
GO:0004435 ISS:UniProtKB F phosphatidylinositol phospholipase C activity
GO:0004629 ISS:UniProtKB F phospholipase C activity
GO:0004871 IEA:UniProtKB-KW F signal transducer activity
GO:0000187 ISS:UniProtKB P activation of MAPK activity
GO:0032835 IEA:Ensembl P glomerulus development
GO:0016042 IEA:UniProtKB-KW P lipid catabolic process
GO:0007200 ISS:UniProtKB P phospholipase C-activating G-protein coupled receptor signaling pathway
GO:0008277 ISS:UniProtKB P regulation of G-protein coupled receptor protein signaling pathway
GO:0045859 ISS:UniProtKB P regulation of protein kinase activity
GO:0046578 ISS:UniProtKB P regulation of Ras protein signal transduction
GO:0007264 IEA:InterPro P small GTPase mediated signal transduction

KEGG Pathway Links

KEGG Pathway ID Description
mmu04024 cAMP signaling pathway
mmu04015 Rap1 signaling pathway
mmu04014 Ras signaling pathway
mmu04919 Thyroid hormone signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR028398 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
IPR000008 C2 domain
IPR011992 EF-hand domain pair
IPR001192 Phosphoinositide phospholipase C family
IPR000909 Phospholipase C, phosphatidylinositol-specific , X domain
IPR001711 Phospholipase C, phosphatidylinositol-specific, Y domain
IPR015359 Phospholipase C, phosphoinositol-specific, EF-hand-like
IPR017946 PLC-like phosphodiesterase, TIM beta/alpha-barrel domain
IPR000159 Ras-association
IPR023578 Ras guanine nucleotide exchange factor domain
IPR001895 Ras guanine-nucleotide exchange factors catalytic domain
IPR029071 Ubiquitin-related domain

UniProt Annotations

Entry Information

Gene Name
phospholipase C, epsilon 1
Protein Entry
PLCE1_MOUSE
UniProt ID
Q8K4S1
Species
Mouse

Comments

Comment Type Description
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. {ECO:0000269|PubMed:12752375}.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
Developmental Stage Specifically expressed in cells committed to the neuronal lineage (at protein level). Weakly expressed at E7, expression strongly increases at later embryonic stages. Expressed abundantly in almost all neural tissues at E12.5 and also detected in tongue muscles, genital tubercle and hand plate. At E15.5 a strong expression in skeletal muscles is detected together with the strong expression in neural tissues. {ECO:0000269|PubMed:12752375}.
Disruption Phenotype Mice exhibit delayed onset and markedly reduced incidence of chemically induced skin squamous tumors. They also display cardiac malformations which mainly affects aortic and pulmonary valves and enhanced susceptibility to cardiac hypertrophy and fibrosis in response to chronic stress. {ECO:0000269|PubMed:15604236, ECO:0000269|PubMed:15743817}.
Domain The Ras-associating domain 1 is degenerated and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane (By similarity). {ECO:0000250}.
Domain The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway (By similarity). {ECO:0000250}.
Enzyme Regulation Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs (By similarity). {ECO:0000250}.
Function The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine- exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation. {ECO:0000269|PubMed:12721365, ECO:0000269|PubMed:15604236, ECO:0000269|PubMed:15743817, ECO:0000269|PubMed:16293787}.
Induction Up-regulated during the differentiation of neural precursor cells into neurons but not glial cells. Up-regulated in heart upon induced hypertrophy. {ECO:0000269|PubMed:12752375, ECO:0000269|PubMed:16293787}.
Sequence Caution Sequence=BAC29099.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
Similarity Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
Similarity Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}.
Similarity Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}.
Similarity Contains 1 Ras-GEF domain. {ECO:0000255|PROSITE- ProRule:PRU00168}.
Similarity Contains 2 Ras-associating domains. {ECO:0000255|PROSITE-ProRule:PRU00166}.
Subcellular Location Cytoplasm, cytosol {ECO:0000250}. Cell membrane {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}. Note=Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Associates with Golgi membranes (By similarity). {ECO:0000250}.
Subunit Interacts with GTP-bound HRAS, RAP1A, RAP2A, RAP2B and RHOA (By similarity). Interacts with IQGAP1. {ECO:0000250, ECO:0000269|PubMed:17086182}.
Tissue Specificity Highly expressed in neurons and to a lower extent in skin, skeletal muscle and heart (at protein level). Expressed in the epidermis. {ECO:0000269|PubMed:15604236, ECO:0000269|PubMed:15743817}.

Identical and Related Proteins

Unique RefSeq proteins for LMP002880 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
134053943 RefSeq NP_062534 2282 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1

Identical Sequences to LMP002880 proteins

Reference Database Accession Length Protein Name
GI:134053943 SwissProt Q8K4S1.3 2282 RecName: Full=1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; AltName: Full=Phosphoinositide phospholipase C-epsilon-1; AltName: Full=Phospholipase C-epsilon-1; Short=PLC-epsilon-1 [Mus musculus]

Related Sequences to LMP002880 proteins

Reference Database Accession Length Protein Name
GI:134053943 DBBJ BAC00906.1 2282 phosphoinositide-specific phospholipase C PLC-epsilon [Mus musculus]
GI:134053943 GenBank AAI45247.1 2296 Plce1 protein [Mus musculus]
GI:134053943 GenBank AAI38350.1 2282 Phospholipase C, epsilon 1 [Mus musculus]
GI:134053943 GenBank AAI38351.1 2282 Phospholipase C, epsilon 1 [Mus musculus]
GI:134053943 RefSeq XP_006527457.1 2298 PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1 [Mus musculus]
GI:134053943 RefSeq XP_008758582.1 2281 PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1 [Rattus norvegicus]