Gene/Proteome Database (LMPD)
LMPD ID
LMP002913
Gene ID
Species
Homo sapiens (Human)
Gene Name
protein kinase C, eta
Gene Symbol
Synonyms
PKC-L; PKCL; PRKCL; nPKC-eta
Alternate Names
protein kinase C eta type
Chromosome
14
Map Location
14q23.1
EC Number
2.7.11.13
Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| protein kinase C eta type | |
|---|---|
| Refseq ID | NP_006246 |
| Protein GI | 28557781 |
| UniProt ID | P24723 |
| mRNA ID | NM_006255 |
| Length | 683 |
| RefSeq Status | REVIEWED |
| MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQINGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDSKIAEQRFGINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKESSKEGNGIGVNSSNRLGIDNFEFIRVLGKGSFGKVMLARVKETGDLYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHDKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHAILRHPFFKEIDWAQLNHRQIEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005911 | IEA:Ensembl | C | cell-cell junction |
| GO:0005737 | IDA:UniProtKB | C | cytoplasm |
| GO:0005829 | ISS:UniProtKB | C | cytosol |
| GO:0070062 | IDA:UniProt | C | extracellular vesicular exosome |
| GO:0005886 | IDA:UniProtKB | C | plasma membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0004699 | IEA:Ensembl | F | calcium-independent protein kinase C activity |
| GO:0019899 | IPI:UniProtKB | F | enzyme binding |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0004697 | TAS:ProtInc | F | protein kinase C activity |
| GO:0007596 | TAS:Reactome | P | blood coagulation |
| GO:0034351 | IMP:UniProtKB | P | negative regulation of glial cell apoptotic process |
| GO:0030168 | TAS:Reactome | P | platelet activation |
| GO:0050861 | IMP:UniProtKB | P | positive regulation of B cell receptor signaling pathway |
| GO:0051092 | IMP:UniProtKB | P | positive regulation of NF-kappaB transcription factor activity |
| GO:0060252 | IMP:UniProtKB | P | positive regulation of glial cell proliferation |
| GO:0045618 | ISS:UniProtKB | P | positive regulation of keratinocyte differentiation |
| GO:0010744 | ISS:UniProtKB | P | positive regulation of macrophage derived foam cell differentiation |
| GO:0070528 | IEA:Ensembl | P | protein kinase C signaling |
| GO:0006468 | IMP:UniProtKB | P | protein phosphorylation |
| GO:2000810 | IMP:UniProtKB | P | regulation of tight junction assembly |
| GO:0007165 | TAS:ProtInc | P | signal transduction |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa04750 | Inflammatory mediator regulation of TRP channels |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR000961 | AGC-kinase, C-terminal |
| IPR000008 | C2 domain |
| IPR020454 | Diacylglycerol/phorbol-ester binding |
| IPR014376 | Protein kinase C, delta/epsilon/eta/theta types |
| IPR027431 | Protein kinase C, eta |
| IPR002219 | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain |
| IPR000719 | Protein kinase domain |
| IPR017441 | Protein kinase, ATP binding site |
| IPR017892 | Protein kinase, C-terminal |
| IPR011009 | Protein kinase-like domain |
| IPR008271 | Serine/threonine-protein kinase, active site |
| IPR002290 | Serine/threonine/dual specificity protein kinase, catalytic domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P24723-1; Sequence=Displayed; Name=2; IsoId=P24723-2; Sequence=VSP_056572; Note=No experimental confirmation available.; |
| Catalytic Activity | ATP + a protein = ADP + a phosphoprotein. |
| Disease | Ischemic stroke (ISCHSTR) [MIM |
| Domain | The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. |
| Enzyme Regulation | Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation. |
| Function | Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1- dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization. {ECO |
| Similarity | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. |
| Similarity | Contains 1 AGC-kinase C-terminal domain. |
| Similarity | Contains 1 C2 domain. {ECO |
| Similarity | Contains 1 protein kinase domain. |
| Similarity | Contains 2 phorbol-ester/DAG-type zinc fingers. |
| Subcellular Location | Cytoplasm . |
| Subunit | Interacts with FYN and RALA (By similarity). Interacts with DGKQ. |
| Tissue Specificity | Most abundant in lung, less in heart and skin. |
Identical and Related Proteins
Unique RefSeq proteins for LMP002913 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 28557781 | RefSeq | NP_006246 | 683 | protein kinase C eta type |
Identical Sequences to LMP002913 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:28557781 | DBBJ | BAF82872.1 | 683 | unnamed protein product [Homo sapiens] |
| GI:28557781 | DBBJ | BAG11427.1 | 683 | protein kinase C eta type, partial [synthetic construct] |
| GI:28557781 | EMBL | CBX47631.1 | 683 | unnamed protein product [Homo sapiens] |
| GI:28557781 | GenBank | AHL57945.1 | 683 | Sequence 422 from patent US 8647822 |
| GI:28557781 | GenBank | AIC62577.1 | 683 | PRKCH, partial [synthetic construct] |
| GI:28557781 | SwissProt | P24723.4 | 683 | RecName: Full=Protein kinase C eta type; AltName: Full=PKC-L; AltName: Full=nPKC-eta [Homo sapiens] |
Related Sequences to LMP002913 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:28557781 | DBBJ | BAG70127.1 | 683 | protein kinase C eta type [Homo sapiens] |
| GI:28557781 | GenBank | ACM82108.1 | 739 | Sequence 7606 from patent US 6812339 |
| GI:28557781 | GenBank | JAB03343.1 | 683 | protein kinase C eta type [Callithrix jacchus] |
| GI:28557781 | RefSeq | XP_001168420.1 | 683 | PREDICTED: protein kinase C eta type isoform X1 [Pan troglodytes] |
| GI:28557781 | RefSeq | XP_003831679.1 | 683 | PREDICTED: protein kinase C eta type isoform X1 [Pan paniscus] |
| GI:28557781 | RefSeq | XP_004055314.1 | 683 | PREDICTED: protein kinase C eta type isoform 1 [Gorilla gorilla gorilla] |