Gene/Proteome Database (LMPD)

LMPD ID
LMP003072
Gene ID
320981
Species
Mus musculus (Mouse)
Gene Name
ectonucleotide pyrophosphatase/phosphodiesterase 6
Gene Symbol
Enpp6
Synonyms
4833421B01Rik; B830047L21Rik; D8Ertd514e; E-NPP 6; Npp6
Alternate Names
ectonucleotide pyrophosphatase/phosphodiesterase family member 6; NPP-6; E-NPP6; GPC-Cpde; glycerophosphocholine cholinephosphodiesterase; choline-specific glycerophosphodiester phosphodiesterase
Chromosome
8
Map Location
8 B1.1|8 26.48 cM
EC Number
3.1.4.-

Proteins

ectonucleotide pyrophosphatase/phosphodiesterase family member 6 precursor
Refseq ID NP_796278
Protein GI 28893409
UniProt ID Q8BGN3
mRNA ID NM_177304
Length 440
RefSeq Status PROVISIONAL
MAAKLWTFLLGFGLSWVWPASAHRKLLVLLLDGFRSDYISEDALASLPGFREIVNRGVKVDYLTPDFPSLSYPNYYTLMTGRHCEVHQMIGNYMWDPRTNKSFDIGVNRDSLMPLWWNGSEPLWITLMKARRKVYMYYWPGCEVEILGVRPTYCLEYKTVPTDINFANAVSDALDSLKSGRADLAAIYHERIDVEGHHYGPSSPQRKDALRAVDTVLKYMIQWIQDRGLQQDLNVILFSDHGMTDIFWMDKVIELSNYISLDDLQQVKDRGPVVSLWPVPGKHSEIYHKLRTVEHMTVYEKESIPNRFYYKKGKFVSPLTLVADEGWFIAESREMLPFWMNSTGKREGWQRGWHGYDNELMDMRGIFLAIGPDFKSNFRAAPIRSVDVYNIMCHVAGITPLPNNGSWSRVVCMLKGQTSSAPPTPLNSCALVLILLLYFV
sig_peptide: 1..22 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2453 peptide sequence: MAAKLWTFLLGFGLSWVWPASA mat_peptide: 23..419 product: Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q8BGN3.1) calculated_mol_wt: 45957 peptide sequence: HRKLLVLLLDGFRSDYISEDALASLPGFREIVNRGVKVDYLTPDFPSLSYPNYYTLMTGRHCEVHQMIGNYMWDPRTNKSFDIGVNRDSLMPLWWNGSEPLWITLMKARRKVYMYYWPGCEVEILGVRPTYCLEYKTVPTDINFANAVSDALDSLKSGRADLAAIYHERIDVEGHHYGPSSPQRKDALRAVDTVLKYMIQWIQDRGLQQDLNVILFSDHGMTDIFWMDKVIELSNYISLDDLQQVKDRGPVVSLWPVPGKHSEIYHKLRTVEHMTVYEKESIPNRFYYKKGKFVSPLTLVADEGWFIAESREMLPFWMNSTGKREGWQRGWHGYDNELMDMRGIFLAIGPDFKSNFRAAPIRSVDVYNIMCHVAGITPLPNNGSWSRVVCMLKGQTS

Gene Information

Entrez Gene ID
320981
Gene Name
ectonucleotide pyrophosphatase/phosphodiesterase 6
Gene Symbol
Enpp6
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031225 IEA:UniProtKB-KW C anchored component of membrane
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0005886 IDA:MGI C plasma membrane
GO:0047390 ISS:UniProtKB F glycerophosphocholine cholinephosphodiesterase activity
GO:0008889 IDA:MGI F glycerophosphodiester phosphodiesterase activity
GO:0008081 ISS:UniProtKB F phosphoric diester hydrolase activity
GO:0019695 IDA:MGI P choline metabolic process
GO:0016042 IEA:UniProtKB-KW P lipid catabolic process
GO:0006629 ISS:UniProtKB P lipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00565 Ether lipid metabolism
mmu00565 Ether lipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR017849 Alkaline phosphatase-like, alpha/beta/alpha
IPR017850 Alkaline-phosphatase-like, core domain
IPR024873 Ectonucleotide pyrophosphatase/phosphodiesterase family
IPR002591 Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase

UniProt Annotations

Entry Information

Gene Name
ectonucleotide pyrophosphatase/phosphodiesterase 6
Protein Entry
ENPP6_MOUSE
UniProt ID
Q8BGN3
Species
Mouse

Comments

Comment Type Description
Catalytic Activity A lysophosphatidylcholine + H(2)O = a monoacylglycerol + phosphocholine.
Catalytic Activity sn-glycero-3-phosphocholine + H(2)O = glycerol + phosphocholine.
Cofactor Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000250};
Enzyme Regulation Inhibited by EDTA and EGTA in vitro. {ECO:0000250}.
Function Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'- monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid (By similarity). {ECO:0000250}.
Similarity Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. {ECO:0000305}.
Subcellular Location Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}.
Subunit Homodimer; disulfide-linked. {ECO:0000250}.
Tissue Specificity Predominantly expressed in kidney with a lesser expression in brain and heart. {ECO:0000269|PubMed:15788404}.

Identical and Related Proteins

Unique RefSeq proteins for LMP003072 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
28893409 RefSeq NP_796278 440 ectonucleotide pyrophosphatase/phosphodiesterase family member 6 precursor

Identical Sequences to LMP003072 proteins

Reference Database Accession Length Protein Name
GI:28893409 DBBJ BAC32873.1 440 unnamed protein product [Mus musculus]
GI:28893409 DBBJ BAC32906.1 440 unnamed protein product [Mus musculus]
GI:28893409 GenBank EDL35588.1 440 ectonucleotide pyrophosphatase/phosphodiesterase 6, isoform CRA_c [Mus musculus]
GI:28893409 SwissProt Q8BGN3.1 440 RecName: Full=Ectonucleotide pyrophosphatase/phosphodiesterase family member 6; Short=E-NPP 6; Short=NPP-6; AltName: Full=Choline-specific glycerophosphodiester phosphodiesterase; AltName: Full=Glycerophosphocholine cholinephosphodiesterase; Short=GPC-Cpde; Flags: Precursor [Mus musculus]

Related Sequences to LMP003072 proteins

Reference Database Accession Length Protein Name
GI:28893409 GenBank AAH96376.1 440 Enpp6 protein [Mus musculus]
GI:28893409 GenBank EDL35586.1 428 ectonucleotide pyrophosphatase/phosphodiesterase 6, isoform CRA_a [Mus musculus]
GI:28893409 GenBank AAI58773.1 440 Enpp6 protein [Rattus norvegicus]
GI:28893409 RefSeq XP_006253218.1 440 PREDICTED: ectonucleotide pyrophosphatase/phosphodiesterase family member 6 isoform X1 [Rattus norvegicus]
GI:28893409 RefSeq XP_007627835.1 440 PREDICTED: ectonucleotide pyrophosphatase/phosphodiesterase family member 6 [Cricetulus griseus]
GI:28893409 SwissProt B0BND0.1 440 RecName: Full=Ectonucleotide pyrophosphatase/phosphodiesterase family member 6; Short=E-NPP 6; Short=NPP-6; AltName: Full=Choline-specific glycerophosphodiester phosphodiesterase; AltName: Full=Glycerophosphocholine cholinephosphodiesterase; Short=GPC-Cpde; Flags: Precursor [Rattus norvegicus]