Gene/Proteome Database (LMPD)

LMPD ID
LMP003133
Gene ID
23908
Species
Mus musculus (Mouse)
Gene Name
heparan sulfate 2-O-sulfotransferase 1
Gene Symbol
Hs2st1
Synonyms
AW214369; Hs2st; mKIAA0448
Alternate Names
heparan sulfate 2-O-sulfotransferase 1; 2OST; 2-OST; 2-O-sulfotransferase
Chromosome
3
Map Location
3 H2|3
EC Number
2.8.2.-

Proteins

heparan sulfate 2-O-sulfotransferase 1
Refseq ID NP_035958
Protein GI 170172560
UniProt ID Q8R3H7
mRNA ID NM_011828
Length 356
RefSeq Status VALIDATED
MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRAKLERAIARHEVREIEQRHTMDGPRQDATLDEEEDIIIIYNRVPKTASTSFTNIAYDLCAKNRYHVLHINTTKNNPVMSLQDQVRFVKNITTWNEMKPGFYHGHISYLDFAKFGVKKKPIYINVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCAPEKLWLQIPFFCGHSSECWNVGSRWAMDQAKSNLINEYFLVGVTEELEDFIMLLEAALPRFFRGATDLYRTGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFIRAHAVREKDGDLYILAQNFFYEKIYPKSN

Gene Information

Entrez Gene ID
23908
Gene Name
heparan sulfate 2-O-sulfotransferase 1
Gene Symbol
Hs2st1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0004394 IMP:MGI F heparan sulfate 2-O-sulfotransferase activity
GO:0015014 IMP:MGI P heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process
GO:0030202 IMP:MGI P heparin metabolic process
GO:0060676 IMP:MGI P ureteric bud formation

KEGG Pathway Links

KEGG Pathway ID Description
ko00534 Glycosaminoglycan biosynthesis - heparan sulfate / heparin
mmu00534 Glycosaminoglycan biosynthesis - heparan sulfate / heparin

REACTOME Pathway Links

REACTOME Pathway ID Description
5893856 HS-GAG biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR007734 Heparan sulphate 2-O-sulfotransferase
IPR027417 P-loop containing nucleoside triphosphate hydrolase
IPR005331 Sulfotransferase

UniProt Annotations

Entry Information

Gene Name
heparan sulfate 2-O-sulfotransferase 1
Protein Entry
HS2ST_MOUSE
UniProt ID
Q8R3H7
Species
Mouse

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=3.7 uM for iduronic acid-containing substrate disaccharide units {ECO:0000269|PubMed:11331020}; KM=19.3 uM for glucuronic acid-containing substrate disaccharide units {ECO:0000269|PubMed:11331020};
Developmental Stage At 7.5 dpc, it is expressed in all three germ layers, although it appears to be more expressed in the embryonic ectoderm and the node. Widespread expression persists at 8.5 dpc, although it is clearly expressed at higher level in rhombomeres 2 and 4 and branchial arches 1 and 2 (which are populated by neural crest from these rhombomeres). At 10.5 dpc, the dorsal and ventral aspects of the neural tube, brain and midbrain-hindbrain junction show the most intense expression. A day later in development, elevated expression is found in the floor plate and the sclerotome. At 12.5 dpc, both the floor plate and the roofplate exhibit strong expression as the mesenchyme of the limb and of the developing whisker follicles. At 13.5 dpc, it is predominantly expressed in embryonic mesenchyme, especially at sites of epithelial-mesenchymal interactions such as the developing teeth and whisker follicles. Strong expression is also apparent in the perichondria of the cartilaginous skeleton, an important site for the regulation of skeletal differentiation.
Disruption Phenotype Mice die in the neonatal period, exhibiting bilateral renal agenesis and defects of the eye and the skeleton. Uronate 2-O-sulfates are not detected in such mice, however, the domain structure of the HS is conserved, due to a compensatory increase in N- and 6-O-sulfation maintain the overall charge density. {ECO:0000269|PubMed:9637690}.
Function Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high- affinity ligand-binding interactions and signaling processes. Required for metanephric development of kidney formation, suggesting that 2-O-sulfation within HS is essential for signaling between ureteric bud and metanephric mesenchyme. Mediates 2-O- sulfation of both L-iduronyl and D-glucuronyl residues. {ECO:0000269|PubMed:11331020, ECO:0000269|PubMed:11457822, ECO:0000269|PubMed:9637690}.
Ptm N-glycosylated. {ECO:0000250}.
Similarity Belongs to the sulfotransferase 3 family. {ECO:0000305}.
Subcellular Location Golgi apparatus membrane {ECO:0000269|PubMed:11687650}; Single-pass type II membrane protein {ECO:0000269|PubMed:11687650}.
Subunit Homotrimer (By similarity). Interacts with the C5- epimerase GLCE. {ECO:0000250, ECO:0000269|PubMed:11687650}.
Tissue Specificity Widely expressed. Expressed at higher level in lung and brain. Weakly expressed in spleen. {ECO:0000269|PubMed:11331020, ECO:0000269|PubMed:9637690}.

Identical and Related Proteins

Unique RefSeq proteins for LMP003133 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
170172560 RefSeq NP_035958 356 heparan sulfate 2-O-sulfotransferase 1

Identical Sequences to LMP003133 proteins

Reference Database Accession Length Protein Name
GI:170172560 DBBJ BAC40511.1 356 unnamed protein product [Mus musculus]
GI:170172560 DBBJ BAE38041.1 356 unnamed protein product [Mus musculus]
GI:170172560 GenBank AAF59900.1 356 heparan sulfate 2-O-sulfotransferase [Mus musculus]
GI:170172560 GenBank AAH59008.1 356 Heparan sulfate 2-O-sulfotransferase 1 [Mus musculus]
GI:170172560 GenBank EDL12030.1 356 heparan sulfate 2-O-sulfotransferase 1 [Mus musculus]
GI:170172560 SwissProt Q8R3H7.2 356 RecName: Full=Heparan sulfate 2-O-sulfotransferase 1; Short=2-O-sulfotransferase; Short=2-OST; Short=2OST [Mus musculus]

Related Sequences to LMP003133 proteins

Reference Database Accession Length Protein Name
GI:170172560 DBBJ BAA20422.1 356 heparan sulfate 2-sulfotransferase, partial [Cricetulus longicaudatus]
GI:170172560 GenBank AAC40135.1 356 heparan sulfate 2-sulfotransferase [Mus musculus]
GI:170172560 GenBank AAH25443.1 356 Heparan sulfate 2-O-sulfotransferase 1 [Mus musculus]
GI:170172560 RefSeq XP_006501512.1 357 PREDICTED: heparan sulfate 2-O-sulfotransferase 1 isoform X1 [Mus musculus]
GI:170172560 RefSeq XP_006986175.1 356 PREDICTED: heparan sulfate 2-O-sulfotransferase 1 [Peromyscus maniculatus bairdii]
GI:170172560 SwissProt O08889.1 356 RecName: Full=Heparan sulfate 2-O-sulfotransferase 1; Short=2-O-sulfotransferase; Short=2OST [Cricetulus longicaudatus]