Gene/Proteome Database (LMPD)
LMPD ID
LMP003139
Gene ID
Species
Homo sapiens (Human)
Gene Name
defender against cell death 1
Gene Symbol
Synonyms
OST2
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DAD-1; oligosaccharyltransferase 2 homolog; oligosaccharyl transferase subunit DAD1; oligosaccharyltransferase subunit 2 (non-catalytic)
Chromosome
14
Map Location
14q11.2
EC Number
2.4.99.18
Summary
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005789 | TAS:Reactome | C | endoplasmic reticulum membrane |
| GO:0070062 | IDA:UniProt | C | extracellular vesicular exosome |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0016020 | IDA:UniProtKB | C | membrane |
| GO:0008250 | ISS:UniProtKB | C | oligosaccharyltransferase complex |
| GO:0004579 | IEA:InterPro | F | dolichyl-diphosphooligosaccharide-protein glycotransferase activity |
| GO:0006915 | IEA:UniProtKB-KW | P | apoptotic process |
| GO:0001824 | IEA:Ensembl | P | blastocyst development |
| GO:0044267 | TAS:Reactome | P | cellular protein metabolic process |
| GO:0043066 | TAS:ProtInc | P | negative regulation of apoptotic process |
| GO:0043687 | TAS:Reactome | P | post-translational protein modification |
| GO:0006486 | ISS:UniProtKB | P | protein glycosylation |
| GO:0018279 | IC:HGNC | P | protein N-linked glycosylation via asparagine |
| GO:0042493 | IEA:Ensembl | P | response to drug |
| GO:0007584 | IEA:Ensembl | P | response to nutrient |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR003038 | DAD/Ost2 |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine. |
| Function | Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis (By similarity). |
| Pathway | Protein modification; protein glycosylation. |
| Similarity | Belongs to the DAD/OST2 family. |
| Subcellular Location | Endoplasmic reticulum membrane {ECO |
| Subunit | Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity). |
| Web Resource | Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/dad1/"; |
Identical and Related Proteins
Unique RefSeq proteins for LMP003139 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 4503253 | RefSeq | NP_001335 | 113 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 |
Identical Sequences to LMP003139 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:4503253 | RefSeq | XP_008063474.1 | 113 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [Tarsius syrichta] |
| GI:4503253 | RefSeq | XP_008533453.1 | 113 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [Equus przewalskii] |
| GI:4503253 | RefSeq | XP_008588297.1 | 113 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 isoform X2 [Galeopterus variegatus] |
| GI:4503253 | RefSeq | XP_008708740.1 | 113 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [Ursus maritimus] |
| GI:4503253 | RefSeq | XP_008838140.1 | 113 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [Nannospalax galili] |
| GI:4503253 | RefSeq | XP_010376647.1 | 113 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [Rhinopithecus roxellana] |
Related Sequences to LMP003139 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:4503253 | GenBank | AAP36473.1 | 114 | Homo sapiens defender against cell death 1, partial [synthetic construct] |
| GI:4503253 | GenBank | AAX29139.1 | 114 | defender against cell death 1, partial [synthetic construct] |
| GI:4503253 | GenBank | AAX29140.1 | 114 | defender against cell death 1, partial [synthetic construct] |
| GI:4503253 | GenBank | AAX29787.1 | 114 | defender against cell death 1, partial [synthetic construct] |
| GI:4503253 | GenBank | AAX36954.1 | 114 | defender against cell death 1, partial [synthetic construct] |
| GI:4503253 | GenBank | ACM85253.1 | 135 | Sequence 10751 from patent US 6812339 |