Gene/Proteome Database (LMPD)

LMPD ID
LMP003172
Gene ID
67216
Species
Mus musculus (Mouse)
Gene Name
membrane bound O-acyltransferase domain containing 2
Gene Symbol
Mboat2
Synonyms
2810049G06Rik; AU022889; AW547221; LPCAT4; Moact2; Oact2
Chromosome
12
Map Location
12 A1.3|12

Proteins

lysophospholipid acyltransferase 2 isoform a
Refseq ID NP_080313
Protein GI 31981088
UniProt ID Q8BN01
mRNA ID NM_026037
Length 519
RefSeq Status VALIDATED
MATTSTTGSTLLQPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIRHVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMQQCCFVFALGYLSVCQITRVYIFDYGQYSADFSGPMMIITQKITSLAYEIHDGMFRKDEELTPSQRGLAVRRMPSLLEYVSYTCNFMGILAGPLCSYKDYIAFIEGRASHVAQPSENGKDEQHGKADPSPNAAVTEKLLVCGLSLLFHLTISNMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLADAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERATFSPTIQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFLEPPQLKLFYDLITWVATQITISYTVVPFVLLSIKPSFTFYSSWYYCLHVCSILVLLLLPVKKSQRRTSTQENVHLSQAKKFDERDNPLGQNSFSTMNNVCNQNRDTGSRHSSLTQ
lysophospholipid acyltransferase 2 isoform b
Refseq ID NP_001076810
Protein GI 134053903
UniProt ID Q8BN01
mRNA ID NM_001083341
Length 487
RefSeq Status VALIDATED
MATTSTTGSTLLQPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIRHVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMQHPMMIITQKITSLAYEIHDGMFRKDEELTPSQRGLAVRRMPSLLEYVSYTCNFMGILAGPLCSYKDYIAFIEGRASHVAQPSENGKDEQHGKADPSPNAAVTEKLLVCGLSLLFHLTISNMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLADAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERATFSPTIQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFLEPPQLKLFYDLITWVATQITISYTVVPFVLLSIKPSFTFYSSWYYCLHVCSILVLLLLPVKKSQRRTSTQENVHLSQAKKFDERDNPLGQNSFSTMNNVCNQNRDTGSRHSSLTQ

Gene Information

Entrez Gene ID
67216
Gene Name
membrane bound O-acyltransferase domain containing 2
Gene Symbol
Mboat2
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0047184 IEA:UniProtKB-EC F 1-acylglycerophosphocholine O-acyltransferase activity
GO:0008654 IEA:UniProtKB-KW P phospholipid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00561 Glycerolipid metabolism
mmu00561 Glycerolipid metabolism
ko00564 Glycerophospholipid metabolism
mmu00564 Glycerophospholipid metabolism
mmu01100 Metabolic pathways
M00089 Triacylglycerol biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5893974 Acyl chain remodelling of PC
5893981 Acyl chain remodelling of PE
5892983 Glycerophospholipid biosynthesis
5892760 Metabolism
5892799 Metabolism of lipids and lipoproteins
5892984 Phospholipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR004299 Membrane bound O-acyl transferase, MBOAT

UniProt Annotations

Entry Information

Gene Name
membrane bound O-acyltransferase domain containing 2
Protein Entry
MBOA2_MOUSE
UniProt ID
Q8BN01
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q8R3I2-1; Sequence=Displayed; Name=2; IsoId=Q8R3I2-2; Sequence=VSP_022458; Name=3; IsoId=Q8R3I2-3; Sequence=VSP_022457, VSP_022459; Note=No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=5.3 uM for oleoyl-CoA (in the presence of LPC C16:0 as cosubstrate) {ECO:0000269|PubMed:18287005}; KM=48.15 uM for oleoyl-CoA (in the presence of LPE C16:0 as cosubstrate) {ECO:0000269|PubMed:18287005}; KM=7.9 uM for LPC C16:0 (in the presence of oleoyl-CoA as cosubstrate) {ECO:0000269|PubMed:18287005}; KM=27.7 uM for LPE C16:0 (in the presence of oleoyl-CoA as cosubstrate) {ECO:0000269|PubMed:18287005}; Vmax=24.24 nmol/min/mg enzyme with oleoyl-CoA and LPC C16:0 as substrates {ECO:0000269|PubMed:18287005}; Vmax=26.3 nmol/min/mg enzyme with oleoyl-CoA and LPE C16:0 as substrates {ECO:0000269|PubMed:18287005};
Catalytic Activity Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.
Catalytic Activity Acyl-CoA + 1-acyl-sn-glycero-3- phosphoethanolamine = CoA + 1,2-diacyl-sn-glycero-3- phosphoethanolamine.
Function Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3- phosphocholine or PC) (LPCAT activity). To a lesser extent, also catalyzes the acylation of lysophosphatidylethanolamine (1-acyl- sn-glycero-3-phosphoethanolamine or LPE) into phosphatidylethanolamine (1,2-diacyl-sn-glycero-3- phosphoethanolamine or PE) (LPEAT activity). Prefers oleoyl-CoA as the acyl donor. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. {ECO:0000269|PubMed:18287005}.
Pathway Lipid metabolism; phospholipid metabolism.
Sequence Caution Sequence=BAC27567.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Similarity Belongs to the membrane-bound acyltransferase family. {ECO:0000305}.
Subcellular Location Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Endoplasmic reticulum {ECO:0000269|PubMed:18287005}.
Tissue Specificity Highly expressed in epididymis, brain, testis, and ovary. {ECO:0000269|PubMed:18287005}.

Identical and Related Proteins

Unique RefSeq proteins for LMP003172 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
31981088 RefSeq NP_080313 519 lysophospholipid acyltransferase 2 isoform a
134053903 RefSeq NP_001076810 487 lysophospholipid acyltransferase 2 isoform b

Identical Sequences to LMP003172 proteins

Reference Database Accession Length Protein Name
GI:134053903 DBBJ BAC36144.1 487 unnamed protein product [Mus musculus]
GI:31981088 DBBJ BAF93902.1 519 lysophospholipid acyltransferase [Mus musculus]
GI:31981088 DBBJ BAG12122.1 519 LPC acyltransferase 4 [Mus musculus]
GI:134053903 GenBank AAH25020.1 487 Mboat2 protein [Mus musculus]
GI:31981088 GenBank ADM04705.1 519 Sequence 43 from patent US 7732155
GI:31981088 GenBank AGM70843.1 519 Sequence 43 from patent US 8383886
GI:31981088 SwissProt Q8R3I2.1 519 RecName: Full=Lysophospholipid acyltransferase 2; Short=LPLAT 2; AltName: Full=1-acylglycerophosphocholine O-acyltransferase; AltName: Full=1-acylglycerophosphoethanolamine O-acyltransferase; AltName: Full=Lysophosphatidylcholine acyltransferase; Short=LPCAT; Short=Lyso-PC acyltransferase; AltName: Full=Lysophosphatidylcholine acyltransferase 4; Short=Lyso-PC acyltransferase 4; Short=mLPCAT4; AltName: Full=Lysophosphatidylethanolamine acyltransferase; Short=LPEAT; Short=Lyso-PE acyltransferase; AltName: Full=Membrane-bound O-acyltransferase domain-containing protein 2; Short=O-acyltransferase domain-containing protein 2 [Mus musculus]

Related Sequences to LMP003172 proteins

Reference Database Accession Length Protein Name
GI:134053903 DBBJ BAB28556.1 487 unnamed protein product [Mus musculus]
GI:134053903 DBBJ BAF93902.1 519 lysophospholipid acyltransferase [Mus musculus]
GI:134053903 DBBJ BAG12122.1 519 LPC acyltransferase 4 [Mus musculus]
GI:31981088 GenBank AAH25020.1 487 Mboat2 protein [Mus musculus]
GI:134053903 GenBank EDL36983.1 487 membrane bound O-acyltransferase domain containing 2, isoform CRA_b [Mus musculus]
GI:31981088 GenBank EDL36985.1 543 membrane bound O-acyltransferase domain containing 2, isoform CRA_d, partial [Mus musculus]
GI:134053903 GenBank ADM04705.1 519 Sequence 43 from patent US 7732155
GI:31981088 RefSeq NP_001101486.2 519 membrane-bound O-acyltransferase domain-containing protein 2 [Rattus norvegicus]
GI:31981088 RefSeq XP_005082068.1 520 PREDICTED: membrane-bound O-acyltransferase domain-containing protein 2-like isoform X1 [Mesocricetus auratus]
GI:31981088 RefSeq XP_005366549.1 521 PREDICTED: lysophospholipid acyltransferase 2 isoform X1 [Microtus ochrogaster]
GI:134053903 SwissProt Q8R3I2.1 519 RecName: Full=Lysophospholipid acyltransferase 2; Short=LPLAT 2; AltName: Full=1-acylglycerophosphocholine O-acyltransferase; AltName: Full=1-acylglycerophosphoethanolamine O-acyltransferase; AltName: Full=Lysophosphatidylcholine acyltransferase; Short=LPCAT; Short=Lyso-PC acyltransferase; AltName: Full=Lysophosphatidylcholine acyltransferase 4; Short=Lyso-PC acyltransferase 4; Short=mLPCAT4; AltName: Full=Lysophosphatidylethanolamine acyltransferase; Short=LPEAT; Short=Lyso-PE acyltransferase; AltName: Full=Membrane-bound O-acyltransferase domain-containing protein 2; Short=O-acyltransferase domain-containing protein 2 [Mus musculus]
GI:31981088 SwissProt Q3T1J2.2 519 RecName: Full=Membrane-bound O-acyltransferase domain-containing protein 2; Short=O-acyltransferase domain-containing protein 2 [Rattus norvegicus]