Gene/Proteome Database (LMPD)

LMPD ID
LMP003187
Gene ID
13200
Species
Mus musculus (Mouse)
Gene Name
dolichyl-di-phosphooligosaccharide-protein glycotransferase
Gene Symbol
Ddost
Synonyms
-
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; DDOST 48 kDa subunit; oligosaccharyl transferase 48 kDa subunit
Chromosome
4
Map Location
4 D3|4
EC Number
2.4.99.18

Proteins

dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor
Refseq ID NP_031864
Protein GI 46195798
UniProt ID O54734
mRNA ID NM_007838
Length 441
RefSeq Status PROVISIONAL
MKMDPRLAVRAWPLCGLLLAVLGCVCASGPRTLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGEMAPPNAYTVTDLVEYSIIIEQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMKEKEKSD
sig_peptide: 1..28 inference: non-experimental evidence, no additional details recorded note: By similarity; propagated from UniProtKB/Swiss-Prot (O54734.2) calculated_mol_wt: 2988 peptide sequence: MKMDPRLAVRAWPLCGLLLAVLGCVCAS mat_peptide: 29..441 product: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (O54734.2) calculated_mol_wt: 46058 peptide sequence: GPRTLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGEMAPPNAYTVTDLVEYSIIIEQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMKEKEKSD

Gene Information

Entrez Gene ID
13200
Gene Name
dolichyl-di-phosphooligosaccharide-protein glycotransferase
Gene Symbol
Ddost
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0008250 ISS:UniProtKB C oligosaccharyltransferase complex
GO:0004579 IEA:InterPro F dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0006486 ISS:UniProtKB P protein glycosylation
GO:0018279 IEA:InterPro P protein N-linked glycosylation via asparagine
GO:0034097 IEA:Ensembl P response to cytokine
GO:0042110 IEA:Ensembl P T cell activation

KEGG Pathway Links

KEGG Pathway ID Description
mmu00510 N-Glycan biosynthesis
mmu04141 Protein processing in endoplasmic reticulum

REACTOME Pathway Links

REACTOME Pathway ID Description
5893865 Advanced glycosylation endproduct receptor signaling

Domain Information

InterPro Annotations

Accession Description
IPR005013 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

UniProt Annotations

Entry Information

Gene Name
dolichyl-di-phosphooligosaccharide-protein glycotransferase
Protein Entry
OST48_MOUSE
UniProt ID
O54734
Species
Mouse

Comments

Comment Type Description
Catalytic Activity Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Function Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Pathway Protein modification; protein glycosylation.
Similarity Belongs to the DDOST 48 kDa subunit family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
Subunit Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity). {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP003187 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
46195798 RefSeq NP_031864 441 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor

Identical Sequences to LMP003187 proteins

Reference Database Accession Length Protein Name
GI:46195798 DBBJ BAC38250.1 441 unnamed protein product [Mus musculus]
GI:46195798 GenBank AAH68132.1 441 Dolichyl-di-phosphooligosaccharide-protein glycotransferase [Mus musculus]
GI:46195798 SwissProt O54734.2 441 RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; Short=DDOST 48 kDa subunit; Short=Oligosaccharyl transferase 48 kDa subunit; Flags: Precursor [Mus musculus]

Related Sequences to LMP003187 proteins

Reference Database Accession Length Protein Name
GI:46195798 DBBJ BAA23671.1 441 oligosaccharyltransferase [Mus musculus]
GI:46195798 DBBJ BAB82434.1 441 dolichyl-diphosphooligosaccharide-protein glycosyltransferase [Mus musculus]
GI:46195798 DBBJ BAE29763.1 441 unnamed protein product [Mus musculus]
GI:46195798 DBBJ BAE28341.1 441 unnamed protein product [Mus musculus]
GI:46195798 GenBank AAH82075.1 441 Dolichyl-diphosphooligosaccharide-protein glycosyltransferase [Rattus norvegicus]
GI:46195798 GenBank EDL13259.1 441 dolichyl-di-phosphooligosaccharide-protein glycotransferase, isoform CRA_c [Mus musculus]