Gene/Proteome Database (LMPD)

LMPD ID
LMP003224
Gene ID
10498
Species
Homo sapiens (Human)
Gene Name
coactivator-associated arginine methyltransferase 1
Gene Symbol
CARM1
Synonyms
PRMT4
Alternate Names
histone-arginine methyltransferase CARM1; protein arginine N-methyltransferase 4
Chromosome
19
Map Location
19p13.2
EC Number
2.1.1.-
Summary
This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013]
Orthologs
View orthologs and multiple alignments for CARM1

Proteins

histone-arginine methyltransferase CARM1
Refseq ID NP_954592
Protein GI 40288288
UniProt ID Q86X55
mRNA ID NM_199141
Length 608
RefSeq Status VALIDATED
MAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDSAGIALYSHEDVCVFKCSVSRETECSRVGKQSFIITLGCNSVLIQFATPNDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPTNTMHYGS

Gene Information

Entrez Gene ID
10498
Gene Name
coactivator-associated arginine methyltransferase 1
Gene Symbol
CARM1
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:UniProtKB C cytoplasm
GO:0005829 ISS:UniProtKB C cytosol
GO:0005654 TAS:Reactome C nucleoplasm
GO:0005634 IDA:UniProtKB C nucleus
GO:0008013 TAS:AgBase F beta-catenin binding
GO:0042054 IDA:UniProtKB F histone methyltransferase activity
GO:0035642 ISS:UniProtKB F histone methyltransferase activity (H3-R17 specific)
GO:0008469 IBA:RefGenome F histone-arginine N-methyltransferase activity
GO:0030374 ISS:UniProtKB F ligand-dependent nuclear receptor transcription coactivator activity
GO:0070577 ISS:UniProtKB F lysine-acetylated histone binding
GO:0008276 ISS:UniProtKB F protein methyltransferase activity
GO:0016274 ISS:UniProtKB F protein-arginine N-methyltransferase activity
GO:0035242 ISS:UniProtKB F protein-arginine omega-N asymmetric methyltransferase activity
GO:0003713 ISS:UniProtKB F transcription coactivator activity
GO:0044212 ISS:UniProtKB F transcription regulatory region DNA binding
GO:0044255 TAS:Reactome P cellular lipid metabolic process
GO:0060350 IEA:Ensembl P endochondral bone morphogenesis
GO:0034971 ISS:UniProtKB P histone H3-R17 methylation
GO:0034970 IMP:UniProtKB P histone H3-R2 methylation
GO:0016571 IDA:UniProtKB P histone methylation
GO:0030520 IEA:Ensembl P intracellular estrogen receptor signaling pathway
GO:0032091 IEA:Ensembl P negative regulation of protein binding
GO:0009405 IEA:InterPro P pathogenesis
GO:0019919 IBA:RefGenome P peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
GO:0008284 IEA:Ensembl P positive regulation of cell proliferation
GO:0045600 ISS:UniProtKB P positive regulation of fat cell differentiation
GO:0003420 IEA:Ensembl P regulation of growth plate cartilage chondrocyte proliferation
GO:0033146 ISS:UniProtKB P regulation of intracellular estrogen receptor signaling pathway
GO:0006355 ISS:UniProtKB P regulation of transcription, DNA-templated
GO:0051591 IEA:Ensembl P response to cAMP
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated
GO:0016032 IEA:UniProtKB-KW P viral process

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_147904 Activation of gene expression by SREBF (SREBP)
REACT_228108 RMTs methylate histone arginines
REACT_200608 Transcriptional activation of mitochondrial biogenesis
REACT_118713 YAP1- and WWTR1 (TAZ)-stimulated gene expression

Domain Information

InterPro Annotations

Accession Description
IPR020989 Histone-arginine methyltransferase CARM1, N-terminal
IPR025799 Protein arginine N-methyltransferase
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase

UniProt Annotations

Entry Information

Gene Name
coactivator-associated arginine methyltransferase 1
Protein Entry
CARM1_HUMAN
UniProt ID
Q86X55
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=3; IsoId=Q86X55-3; Sequence=Displayed; Name=1; IsoId=Q86X55-1; Sequence=VSP_039876; Name=2; IsoId=Q86X55-2; Sequence=VSP_012506, VSP_012507; Note=No experimental confirmation available.;
Catalytic Activity S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone].
Enzyme Regulation Methylation of H3R17 (H3R17me) by CARM1 is stimulated by preacetylation of H3 'Lys-18' (H3K18ac) H3 'Lys-23' (H3K23ac) by EP300 and blocked by citrullination of H3 'Arg-17' (H3R17ci) by PADI4.
Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs.
Interaction P17676:CEBPB; NbExp=2; IntAct=EBI-2339854, EBI-969696; Q05826:CEBPB (xeno); NbExp=3; IntAct=EBI-2339854, EBI-7774198; Q96EY1:DNAJA3; NbExp=2; IntAct=EBI-2339854, EBI-356767; O43809:NUDT21; NbExp=2; IntAct=EBI-2339854, EBI-355720; Q96PU8:QKI; NbExp=2; IntAct=EBI-2339854, EBI-945792; Q92922:SMARCC1; NbExp=4; IntAct=EBI-2339854, EBI-355653;
Ptm Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity).
Ptm Phosphorylation at Ser-216 interferes with S-adenosyl-L- methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol. {ECO
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family.
Similarity Contains 1 SAM-dependent MTase PRMT-type domain.
Subcellular Location Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
Subunit Homodimer (Probable). Interacts with the C-terminus of NCOA2/GRIP1, NCO3/ACTR and NCOA1/SRC1. Part of a complex consisting of CARM1, EP300/P300 and NCOA2/GRIP1. Interacts with FLII, TP53, myogenic factor MEF2, EP300/P300, TRIM24, CREBBP and CTNNB1. Identified in a complex containing CARM1, TRIM24 and NCOA2/GRIP1. Interacts with NCOA3/SRC3. Interacts with SNRPC (By similarity). Interacts with NR1H4. Interacts with RELA. Interacts with HTLV-1 Tax-1. {ECO
Tissue Specificity Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

Identical and Related Proteins

Unique RefSeq proteins for LMP003224 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
40288288 RefSeq NP_954592 608 histone-arginine methyltransferase CARM1

Identical Sequences to LMP003224 proteins

Reference Database Accession Length Protein Name
GI:40288288 GenBank ADA20807.1 608 Sequence 2631 from patent US 7608413
GI:40288288 GenBank ADT50100.1 608 Sequence 2117 from patent US 7842467
GI:40288288 GenBank ADT50101.1 608 Sequence 2118 from patent US 7842467
GI:40288288 GenBank AEN36702.1 608 Sequence 2630 from patent US 7998689
GI:40288288 GenBank AEN36703.1 608 Sequence 2631 from patent US 7998689
GI:40288288 SwissProt Q86X55.3 608 RecName: Full=Histone-arginine methyltransferase CARM1; AltName: Full=Coactivator-associated arginine methyltransferase 1; AltName: Full=Protein arginine N-methyltransferase 4 [Homo sapiens]

Related Sequences to LMP003224 proteins

Reference Database Accession Length Protein Name
GI:40288288 DBBJ BAE16333.1 608 coactivator-associated arginine methyltransferase 1 variant 1 [Rattus norvegicus]
GI:40288288 GenBank AAD41265.2 608 protein arginine methyltransferase [Mus musculus]
GI:40288288 GenBank ABJ31080.1 608 Sequence 8 from patent US 7098015
GI:40288288 GenBank ACQ18155.1 608 Sequence 69 from patent US 7510850
GI:40288288 RefSeq NP_067506.2 608 histone-arginine methyltransferase CARM1 isoform 1 [Mus musculus]
GI:40288288 RefSeq NP_001025212.1 608 histone-arginine methyltransferase CARM1 isoform a [Rattus norvegicus]