Gene/Proteome Database (LMPD)

LMPD ID
LMP003387
Gene ID
27163
Species
Homo sapiens (Human)
Gene Name
N-acylethanolamine acid amidase
Gene Symbol
NAAA
Synonyms
ASAHL; PLT
Alternate Names
N-acylethanolamine-hydrolyzing acid amidase; ASAH-like protein; acid ceramidase-like protein
Chromosome
4
Map Location
4q21.1
EC Number
3.5.1.-
Summary
This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for NAAA

Proteins

N-acylethanolamine-hydrolyzing acid amidase isoform 1 precursor
Refseq ID NP_055250
Protein GI 109148550
UniProt ID Q02083
mRNA ID NM_014435
Length 359
RefSeq Status REVIEWED
MRTADREARPGLPSLLLLLLAGAGLSAASPPAAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLIGKVVLELERFLPQPFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGRNLDYPFGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKFTVSGDERDKGWWWENAIAALFRRHIPVSWLIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIKALNATGQANLSLEALFQILSVVPVYNNFTIYTTVMSAGSPDKYMTRIRNPSRK
sig_peptide: 1..28 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2834 peptide sequence: MRTADREARPGLPSLLLLLLAGAGLSAA mat_peptide: 29..323 product: N-acylethanolamine-hydrolyzing acid amidase isoform 2 calculated_mol_wt: 33147 peptide sequence: SPPAAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLIGKVVLELERFLPQPFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGRNLDYPFGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKFTVSGDERDKGWWWENAIAALFRRHIPVSWLIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIKALNATGQANLSLEALFQ sig_peptide: 1..28 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2834 peptide sequence: MRTADREARPGLPSLLLLLLAGAGLSAA mat_peptide: 29..359 product: N-acylethanolamine-hydrolyzing acid amidase isoform 1 calculated_mol_wt: 37250 peptide sequence: SPPAAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLIGKVVLELERFLPQPFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGRNLDYPFGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKFTVSGDERDKGWWWENAIAALFRRHIPVSWLIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIKALNATGQANLSLEALFQILSVVPVYNNFTIYTTVMSAGSPDKYMTRIRNPSRK
N-acylethanolamine-hydrolyzing acid amidase isoform 2 precursor
Refseq ID NP_001035861
Protein GI 109148548
UniProt ID Q02083
mRNA ID NM_001042402
Length 323
RefSeq Status REVIEWED
MRTADREARPGLPSLLLLLLAGAGLSAASPPAAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLIGKVVLELERFLPQPFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGRNLDYPFGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKFTVSGDERDKGWWWENAIAALFRRHIPVSWLIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIKALNATGQANLSLEALFQ

Gene Information

Entrez Gene ID
27163
Gene Name
N-acylethanolamine acid amidase
Gene Symbol
NAAA
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:MGI C cytoplasm
GO:0070062 IDA:UniProtKB C extracellular vesicular exosome
GO:0005764 IEA:UniProtKB-KW C lysosome
GO:0016810 IDA:MGI F hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
GO:0008134 IPI:UniProtKB F transcription factor binding
GO:0006629 IEA:InterPro P lipid metabolic process

Domain Information

InterPro Annotations

Accession Description
IPR029130 Acid ceramidase, N-terminal
IPR016699 Acid_ceramidase-like
IPR029132 Choloylglycine hydrolase/NAAA C-terminal

UniProt Annotations

Entry Information

Gene Name
N-acylethanolamine acid amidase
Protein Entry
NAAA_HUMAN
UniProt ID
Q02083
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q02083-1; Sequence=Displayed; Name=2; IsoId=Q02083-2; Sequence=VSP_000330; Name=3; IsoId=Q02083-3; Sequence=VSP_000328, VSP_000329; Note=No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=97 uM for N-palmitoylethanolamine ; pH dependence: Optimum pH is 4.5 with N-palmitoylethanolamine as substrate. ;
Enzyme Regulation Stimulated by DTT and Nonidet P-40.
Function Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N- palmitoylethanolamine > N-myristoylethanolamine > N- lauroylethanolamine = N-stearoylethanolamine > N- arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N- lauroylsphingosine and N-palmitoylsphingosine.
Ptm Autoproteolytic cleavage in the acidic lumen of the lysosome activates the enzyme.
Ptm N-glycosylated. Tunicamycin treatment causes a reduction in specific activity against N-palmitoylethanolamine. {ECO
Similarity Belongs to the acid ceramidase family.
Subcellular Location Lysosome {ECO
Subunit Heterodimer of an alpha and a beta subunit, non- covalently linked.
Tissue Specificity Expressed in numerous tissues, with highest levels in liver and kidney, followed by pancreas.

Identical and Related Proteins

Unique RefSeq proteins for LMP003387 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
109148550 RefSeq NP_055250 359 N-acylethanolamine-hydrolyzing acid amidase isoform 1 precursor
109148548 RefSeq NP_001035861 323 N-acylethanolamine-hydrolyzing acid amidase isoform 2 precursor

Identical Sequences to LMP003387 proteins

Reference Database Accession Length Protein Name
GI:109148550 DBBJ BAD88528.1 359 N-acylethanolamine-hydrolyzing acid amidase [Homo sapiens]
GI:109148548 GenBank EAX05756.1 323 N-acylsphingosine amidohydrolase (acid ceramidase)-like, isoform CRA_a [Homo sapiens]
GI:109148550 GenBank EAX05757.1 359 N-acylsphingosine amidohydrolase (acid ceramidase)-like, isoform CRA_b [Homo sapiens]
GI:109148548 GenBank EAX05760.1 323 N-acylsphingosine amidohydrolase (acid ceramidase)-like, isoform CRA_e [Homo sapiens]
GI:109148550 RefSeq XP_005262977.1 359 PREDICTED: N-acylethanolamine-hydrolyzing acid amidase isoform X1 [Homo sapiens]
GI:109148548 RefSeq XP_005262982.1 323 PREDICTED: N-acylethanolamine-hydrolyzing acid amidase isoform X6 [Homo sapiens]
GI:109148550 RefSeq XP_006714242.1 359 PREDICTED: N-acylethanolamine-hydrolyzing acid amidase isoform X8 [Homo sapiens]
GI:109148550 SwissProt Q02083.3 359 RecName: Full=N-acylethanolamine-hydrolyzing acid amidase; AltName: Full=Acid ceramidase-like protein; AltName: Full=N-acylsphingosine amidohydrolase-like; Short=ASAH-like protein; Contains: RecName: Full=N-acylethanolamine-hydrolyzing acid amidase subunit alpha; Contains: RecName: Full=N-acylethanolamine-hydrolyzing acid amidase subunit beta; Flags: Precursor [Homo sapiens]

Related Sequences to LMP003387 proteins

Reference Database Accession Length Protein Name
GI:109148548 DBBJ BAD88528.1 359 N-acylethanolamine-hydrolyzing acid amidase [Homo sapiens]
GI:109148550 EMBL CAJ42761.1 359 unnamed protein product [Homo sapiens]
GI:109148548 EMBL CAJ42761.1 359 unnamed protein product [Homo sapiens]
GI:109148548 GenBank EAX05757.1 359 N-acylsphingosine amidohydrolase (acid ceramidase)-like, isoform CRA_b [Homo sapiens]
GI:109148550 GenBank EAX05759.1 362 N-acylsphingosine amidohydrolase (acid ceramidase)-like, isoform CRA_d [Homo sapiens]
GI:109148550 GenBank JAA37657.1 358 N-acylethanolamine acid amidase [Pan troglodytes]
GI:109148548 RefSeq NP_055250.2 359 N-acylethanolamine-hydrolyzing acid amidase isoform 1 precursor [Homo sapiens]
GI:109148550 RefSeq XP_001152628.2 358 PREDICTED: N-acylethanolamine-hydrolyzing acid amidase isoform X1 [Pan troglodytes]
GI:109148550 RefSeq XP_006714241.1 366 PREDICTED: N-acylethanolamine-hydrolyzing acid amidase isoform X7 [Homo sapiens]
GI:109148548 RefSeq XP_006714242.1 359 PREDICTED: N-acylethanolamine-hydrolyzing acid amidase isoform X8 [Homo sapiens]
GI:109148550 RefSeq XP_009445990.1 358 PREDICTED: N-acylethanolamine-hydrolyzing acid amidase isoform X1 [Pan troglodytes]
GI:109148548 SwissProt Q02083.3 359 RecName: Full=N-acylethanolamine-hydrolyzing acid amidase; AltName: Full=Acid ceramidase-like protein; AltName: Full=N-acylsphingosine amidohydrolase-like; Short=ASAH-like protein; Contains: RecName: Full=N-acylethanolamine-hydrolyzing acid amidase subunit alpha; Contains: RecName: Full=N-acylethanolamine-hydrolyzing acid amidase subunit beta; Flags: Precursor [Homo sapiens]