Gene/Proteome Database (LMPD)

LMPD ID
LMP003541
Gene ID
1650
Species
Homo sapiens (Human)
Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Gene Symbol
DDOST
Synonyms
AGER1; CDG1R; OKSWcl45; OST; OST48; WBP1
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; oligosaccharyltransferase subunit 48; advanced glycation endproduct receptor 1; oligosaccharyltransferase 48 kDa subunit; oligosaccharyl transferase 48 kDa subunit; dolichyl-diphosphooligosaccharide-protein glycotransferase
Chromosome
1
Map Location
1p36.1
EC Number
2.4.99.18
Summary
This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for DDOST

Proteins

dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor
Refseq ID NP_005207
Protein GI 20070197
UniProt ID P39656
mRNA ID NM_005216
Length 456
RefSeq Status REVIEWED
MGYFRCAGAGSFGRRRKMEPSTAARAWALFWLLLPLLGAVCASGPRTLVLLDNLNVRETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMLGLFIFSIVFLHMKEKEKSD
sig_peptide: 1..42 calculated_mol_wt: 4604 peptide sequence: MGYFRCAGAGSFGRRRKMEPSTAARAWALFWLLLPLLGAVCA mat_peptide: 43..456 product: dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit calculated_mol_wt: 46116 peptide sequence: SGPRTLVLLDNLNVRETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMLGLFIFSIVFLHMKEKEKSD

Gene Information

Entrez Gene ID
1650
Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Gene Symbol
DDOST
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:HPA C endoplasmic reticulum
GO:0005789 TAS:Reactome C endoplasmic reticulum membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0043231 IDA:UniProtKB C intracellular membrane-bounded organelle
GO:0016020 IDA:UniProtKB C membrane
GO:0008250 ISS:UniProtKB C oligosaccharyltransferase complex
GO:0043234 IDA:UniProtKB C protein complex
GO:0004579 IEA:InterPro F dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0006614 TAS:Reactome P SRP-dependent cotranslational protein targeting to membrane
GO:0042110 IDA:UniProtKB P T cell activation
GO:0044267 TAS:Reactome P cellular protein metabolic process
GO:0010467 TAS:Reactome P gene expression
GO:0045087 TAS:Reactome P innate immune response
GO:0043687 TAS:Reactome P post-translational protein modification
GO:0006487 IDA:UniProtKB P protein N-linked glycosylation
GO:0018279 IC:HGNC P protein N-linked glycosylation via asparagine
GO:0006486 ISS:UniProtKB P protein glycosylation
GO:0034097 IDA:UniProtKB P response to cytokine
GO:0006412 TAS:Reactome P translation

KEGG Pathway Links

KEGG Pathway ID Description
hsa00510 N-Glycan biosynthesis
hsa04141 Protein processing in endoplasmic reticulum

Domain Information

InterPro Annotations

Accession Description
IPR005013 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

UniProt Annotations

Entry Information

Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Protein Entry
OST48_HUMAN
UniProt ID
P39656
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P39656-1; Sequence=Displayed; Name=2; IsoId=P39656-2; Sequence=VSP_055498; Note=No experimental confirmation available.; Name=3; IsoId=P39656-3; Sequence=VSP_055499; Note=No experimental confirmation available.;
Catalytic Activity Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Caution It is uncertain whether Met-1 or Met-18 is the initiator.
Disease Congenital disorder of glycosylation 1R (CDG1R) [MIM
Function Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Interaction Q86WV6:TMEM173; NbExp=2; IntAct=EBI-358866, EBI-2800345;
Pathway Protein modification; protein glycosylation.
Sequence Caution Sequence=BAB93478.1; Type=Erroneous initiation; Evidence= ;
Similarity Belongs to the DDOST 48 kDa subunit family.
Subcellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Subunit Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome (By similarity). Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex.

Identical and Related Proteins

Unique RefSeq proteins for LMP003541 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
20070197 RefSeq NP_005207 456 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor

Identical Sequences to LMP003541 proteins

Reference Database Accession Length Protein Name
GI:20070197 GenBank ADT48671.1 456 Sequence 688 from patent US 7842467
GI:20070197 GenBank ADT48672.1 456 Sequence 689 from patent US 7842467
GI:20070197 GenBank AEI19337.1 456 Sequence 666 from patent US 7960100
GI:20070197 GenBank AEI19338.1 456 Sequence 667 from patent US 7960100
GI:20070197 GenBank AIC54271.1 456 DDOST, partial [synthetic construct]
GI:20070197 RefSeq XP_008965314.1 456 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit [Pan paniscus]

Related Sequences to LMP003541 proteins

Reference Database Accession Length Protein Name
GI:20070197 DBBJ BAG09609.1 456 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor, partial [synthetic construct]
GI:20070197 GenBank AAP36629.1 457 Homo sapiens dolichyl-diphosphooligosaccharide-protein glycosyltransferase, partial [synthetic construct]
GI:20070197 GenBank AAX43758.1 457 dolichyl-diphosphooligosaccharide-protein glycosyltransferase, partial [synthetic construct]
GI:20070197 GenBank AAX43759.1 457 dolichyl-diphosphooligosaccharide-protein glycosyltransferase, partial [synthetic construct]
GI:20070197 GenBank ADT47063.1 456 Sequence 724 from patent US 7842466
GI:20070197 GenBank ADT48673.1 456 Sequence 690 from patent US 7842467