Gene/Proteome Database (LMPD)

LMPD ID
LMP003575
Gene ID
94212
Species
Mus musculus (Mouse)
Gene Name
phosphoprotein associated with glycosphingolipid microdomains 1
Gene Symbol
Pag1
Synonyms
Cbp; F730007C19Rik; Pag
Chromosome
3
Map Location
3 A1|3

Proteins

phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Refseq ID NP_001181960
Protein GI 304307779
UniProt ID Q3U1F9
mRNA ID NM_001195031
Length 429
RefSeq Status VALIDATED
MGPAGSVLSSGQMQMQMVLWGSLAAVAMFFLITFLVLLCSTCDREKKPRQHSGDHENLMNVPSDKDMFSHSATSLTTDALASSEQNGVLTNGDILSEDSTLTCMQHYEEVQTSASDLLDSQDSTGKAKCHQSRELPRIPPENAVDEILTARAADTELGPGVEGPYEVLKDSSSQENMVEDCLYETVKEIKEVADKGQGGKSKSTSALKELQGAPMEGKADFAEYASVDRNKKCRHSANAESILGTCSDLDEESPPPVPVKLLDENANLPQEGGGQAEEQAAEGTGGHSKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQSAHKPGPCMKGPESACHSMKGLPQRSSSSCNDLYATVKDFEKTPNSISTLPPARRPSEEPEPDYEAIQTLNREDEKVPLETNGHHVPKESDYESIGDLQQCRDVTRL
phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Refseq ID NP_444412
Protein GI 31543456
UniProt ID Q3U1F9
mRNA ID NM_053182
Length 429
RefSeq Status VALIDATED
Protein sequence is identical to GI:304307779 (mRNA isoform)

Gene Information

Entrez Gene ID
94212
Gene Name
phosphoprotein associated with glycosphingolipid microdomains 1
Gene Symbol
Pag1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0045121 IEA:Ensembl C membrane raft
GO:0045121 TAS:HGNC C membrane raft
GO:0005886 IEA:Ensembl C plasma membrane
GO:0005886 TAS:HGNC C plasma membrane
GO:0002376 IEA:UniProtKB-KW P immune system process
GO:0035556 IEA:Ensembl P intracellular signal transduction
GO:0035556 TAS:HGNC P intracellular signal transduction
GO:0050868 IDA:HGNC P negative regulation of T cell activation
GO:0050863 IEA:Ensembl P regulation of T cell activation
GO:0050863 TAS:HGNC P regulation of T cell activation

REACTOME Pathway Links

REACTOME Pathway ID Description
5892925 Adaptive Immune System
5892763 Disease
5893394 GAB1 signalosome
5892920 Immune System
5893547 Phosphorylation of CD3 and TCR zeta chains
5892891 Signal Transduction
5892913 Signaling by EGFR
5892914 Signaling by EGFR in Cancer
5893546 TCR signaling

Domain Information

InterPro Annotations

Accession Description

UniProt Annotations

Entry Information

Gene Name
phosphoprotein associated with glycosphingolipid microdomains 1
Protein Entry
PHAG1_MOUSE
UniProt ID
Q3U1F9
Species
Mouse

Comments

Comment Type Description
Disruption Phenotype Mice are viable and do not show any developmental defect up to 20 months of age. They have normal T- cell development and normal T- and B-cell responses. {ECO:0000269|PubMed:16166631}.
Function Negatively regulates TCR (T-cell antigen receptor)- mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. {ECO:0000269|PubMed:12218089, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:16166631}.
Ptm Palmitoylated. {ECO:0000269|PubMed:12626544}.
Ptm Phosphorylated by FYN on Tyr-314 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. {ECO:0000269|PubMed:11777944, ECO:0000269|PubMed:12218089, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:14967142, ECO:0000269|PubMed:17947660, ECO:0000269|PubMed:19144319}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:11777944, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:16166631}; Single-pass type III membrane protein {ECO:0000269|PubMed:11777944, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:16166631}. Note=Present in lipid rafts.
Subunit When phosphorylated, interacts with CSK. Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. {ECO:0000269|PubMed:11777944, ECO:0000269|PubMed:12218089, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14967142}.
Tissue Specificity Present in T-cells (at protein level). {ECO:0000269|PubMed:16166631}.

Identical and Related Proteins

Unique RefSeq proteins for LMP003575 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
304307779 RefSeq NP_001181960 429 phosphoprotein associated with glycosphingolipid-enriched microdomains 1

Identical Sequences to LMP003575 proteins

Reference Database Accession Length Protein Name
GI:304307779 GenBank AAI45764.1 429 Pag1 protein [Mus musculus]
GI:304307779 GenBank AAI45762.1 429 Pag1 protein [Mus musculus]
GI:304307779 GenBank AAI45329.1 429 Pag1 protein [Mus musculus]
GI:304307779 RefSeq XP_006530170.1 429 PREDICTED: phosphoprotein associated with glycosphingolipid-enriched microdomains 1 isoform X1 [Mus musculus]
GI:304307779 RefSeq XP_006530171.1 429 PREDICTED: phosphoprotein associated with glycosphingolipid-enriched microdomains 1 isoform X2 [Mus musculus]
GI:304307779 RefSeq XP_006530172.1 429 PREDICTED: phosphoprotein associated with glycosphingolipid-enriched microdomains 1 isoform X3 [Mus musculus]

Related Sequences to LMP003575 proteins

Reference Database Accession Length Protein Name
GI:304307779 DBBJ BAA95413.1 424 Csk binding protein Cbp [Rattus norvegicus]
GI:304307779 DBBJ BAE33539.1 429 unnamed protein product [Mus musculus]
GI:304307779 GenBank AAG44565.1 429 phosphoprotein-associated with GEMs [Mus musculus]
GI:304307779 GenBank EDL05180.1 439 phosphoprotein associated with glycosphingolipid microdomains 1, isoform CRA_b, partial [Mus musculus]
GI:304307779 RefSeq NP_071589.1 424 phosphoprotein associated with glycosphingolipid-enriched microdomains 1 [Rattus norvegicus]
GI:304307779 SwissProt Q9JM80.1 424 RecName: Full=Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; AltName: Full=Csk-binding protein; AltName: Full=Transmembrane phosphoprotein Cbp [Rattus norvegicus]