Gene/Proteome Database (LMPD)

LMPD ID
LMP003623
Gene ID
231999
Species
Mus musculus (Mouse)
Gene Name
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8
Gene Symbol
Plekha8
Synonyms
6330400G01; AA517676; BC052360; Fapp-2; Fapp2
Alternate Names
pleckstrin homology domain-containing family A member 8; PH domain-containing family A member 8; phosphoinositol 4-phosphate adapter protein 2; phosphatidylinositol-four-phosphate adapter protein 2
Chromosome
6
Map Location
6 B3|6

Proteins

pleckstrin homology domain-containing family A member 8 isoform 1
Refseq ID NP_001157833
Protein GI 256355025
UniProt ID Q80W71
mRNA ID NM_001164361
Length 519
RefSeq Status VALIDATED
MEGVLYKWTNYLSGWQPRWFLLCGGILSYYDSPEDAWKGCKGSIQMAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVATAGVTDSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYHTPPGSPQLAVLKSSKMKHPIIPIHNSLERSMELNSCENGSLSIEVNGDEEILMKTKSSLYLKSTEVDCSISSEENTDDNVTVQGEIMKEDGEENLESHDKDPAQPGSDSVCSPESPWEDNEEVIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFALALRAAPSYEDFVAALTIKEGDHQKEAFSAGMQRDLSLYLPAMEKQLAILDTLYEIHGLESDEVV
pleckstrin homology domain-containing family A member 8 isoform 2
Refseq ID NP_001001335
Protein GI 47716514
UniProt ID Q80W71
mRNA ID NM_001001335
Length 474
RefSeq Status VALIDATED
MAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVATAGVTDSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYHTPPGSPQLAVLKSSKMKHPIIPIHNSLERSMELNSCENGSLSIEVNGDEEILMKTKSSLYLKSTEVDCSISSEENTDDNVTVQGEIMKEDGEENLESHDKDPAQPGSDSVCSPESPWEDNEEVIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFALALRAAPSYEDFVAALTIKEGDHQKEAFSAGMQRDLSLYLPAMEKQLAILDTLYEIHGLESDEVV

Gene Information

Entrez Gene ID
231999
Gene Name
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8
Gene Symbol
Plekha8
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0005802 ISS:UniProtKB C trans-Golgi network
GO:0097001 ISS:UniProtKB F ceramide binding
GO:0051861 ISS:UniProtKB F glycolipid binding
GO:0017089 ISS:UniProtKB F glycolipid transporter activity
GO:0070273 ISS:UniProtKB F phosphatidylinositol-4-phosphate binding
GO:0035621 ISS:UniProtKB P ER to Golgi ceramide transport
GO:0006869 ISS:UniProtKB P lipid transport

Domain Information

InterPro Annotations

Accession Description
IPR014830 Glycolipid transfer protein domain
IPR001849 Pleckstrin homology domain
IPR011993 Pleckstrin homology-like domain

UniProt Annotations

Entry Information

Gene Name
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8
Protein Entry
PKHA8_MOUSE
UniProt ID
Q80W71
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q80W71-1; Sequence=Displayed; Name=2; IsoId=Q80W71-2; Sequence=VSP_028547; Note=No experimental confirmation available.;
Domain The PH domain of FAPPS binds the small GTPase ARF1 and phosphatidylinositol-4-phosphate (PtdIns4P) with high selectivity, and is required for recruitment of FAPPs to the trans-Golgi network (TGN). {ECO:0000250}.
Function Cargo transport protein that is required for apical transport from the trans-Golgi network (TGN). Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4- phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation (By similarity). {ECO:0000250}.
Similarity Contains 1 PH domain. {ECO:0000255|PROSITE- ProRule:PRU00145}.
Subcellular Location Cytoplasm {ECO:0000250}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250}. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Mainly localized to the trans-Golgi network (TGN) but also found at the rims of Golgi cisternae. Associates with ARF1 at Golgi apparatus membranes (By similarity). {ECO:0000250}.
Subunit Homodimer. Interacts with ARF1; the interaction together with phosphatidylinositol 4-phosphate binding is required for FAPP2 GlcCer transfer ability. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP003623 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
256355025 RefSeq NP_001157833 519 pleckstrin homology domain-containing family A member 8 isoform 1
47716514 RefSeq NP_001001335 474 pleckstrin homology domain-containing family A member 8 isoform 2

Identical Sequences to LMP003623 proteins

Reference Database Accession Length Protein Name
GI:47716514 GenBank AAH52360.1 474 Pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 [Mus musculus]
GI:256355025 GenBank EDK98709.1 519 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8, isoform CRA_b [Mus musculus]
GI:256355025 SwissProt Q80W71.2 519 RecName: Full=Pleckstrin homology domain-containing family A member 8; Short=PH domain-containing family A member 8; AltName: Full=Phosphatidylinositol-four-phosphate adapter protein 2; Short=FAPP-2; Short=Phosphoinositol 4-phosphate adapter protein 2 [Mus musculus]

Related Sequences to LMP003623 proteins

Reference Database Accession Length Protein Name
GI:256355025 GenBank EDK98708.1 522 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8, isoform CRA_a, partial [Mus musculus]
GI:47716514 GenBank EDK98708.1 522 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8, isoform CRA_a, partial [Mus musculus]
GI:47716514 GenBank EDK98709.1 519 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8, isoform CRA_b [Mus musculus]
GI:256355025 GenBank EDL88113.1 520 similar to phosphoinositol 4-phosphate adaptor protein-2 (predicted) [Rattus norvegicus]
GI:256355025 RefSeq NP_001102705.1 520 pleckstrin homology domain-containing family A member 8 [Rattus norvegicus]
GI:47716514 RefSeq NP_001102705.1 520 pleckstrin homology domain-containing family A member 8 [Rattus norvegicus]
GI:47716514 RefSeq NP_001157833.1 519 pleckstrin homology domain-containing family A member 8 isoform 1 [Mus musculus]
GI:256355025 RefSeq XP_003935224.1 520 PREDICTED: pleckstrin homology domain-containing family A member 8 isoform X1 [Saimiri boliviensis boliviensis]
GI:256355025 RefSeq XP_005360893.1 519 PREDICTED: pleckstrin homology domain-containing family A member 8 [Microtus ochrogaster]
GI:47716514 SwissProt Q80W71.2 519 RecName: Full=Pleckstrin homology domain-containing family A member 8; Short=PH domain-containing family A member 8; AltName: Full=Phosphatidylinositol-four-phosphate adapter protein 2; Short=FAPP-2; Short=Phosphoinositol 4-phosphate adapter protein 2 [Mus musculus]
GI:47716514 SwissProt D3ZY60.1 520 RecName: Full=Pleckstrin homology domain-containing family A member 8; Short=PH domain-containing family A member 8; AltName: Full=Phosphatidylinositol-four-phosphate adapter protein 2; Short=FAPP-2; Short=Phosphoinositol 4-phosphate adapter protein 2 [Rattus norvegicus]
GI:256355025 SwissProt D3ZY60.1 520 RecName: Full=Pleckstrin homology domain-containing family A member 8; Short=PH domain-containing family A member 8; AltName: Full=Phosphatidylinositol-four-phosphate adapter protein 2; Short=FAPP-2; Short=Phosphoinositol 4-phosphate adapter protein 2 [Rattus norvegicus]