Gene/Proteome Database (LMPD)
LMPD ID
LMP003635
Gene ID
Species
Homo sapiens (Human)
Gene Name
STT3A, subunit of the oligosaccharyltransferase complex (catalytic)
Gene Symbol
Synonyms
ITM1; STT3-A; TMC
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A; B5; transmembrane conserved; transmembrane protein TMC; integral membrane protein 1; integral transmembrane protein 1; oligosaccharyl transferase subunit STT3A; dolichyl-diphosphooligosaccharide protein glycotransferase; STT3A, cataylic subunit of the oligosaccharyltransferase complex; STT3, subunit of the oligosaccharyltransferase complex, homolog A
Chromosome
11
Map Location
11q23.3
EC Number
2.4.99.18
Proteins
| dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform a | |
|---|---|
| Refseq ID | NP_689926 |
| Protein GI | 22749415 |
| UniProt ID | P46977 |
| mRNA ID | NM_152713 |
| Length | 705 |
| RefSeq Status | VALIDATED |
| MTKFGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSICWAAKCALAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT | |
| dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform a | |
|---|---|
| Refseq ID | NP_001265432 |
| Protein GI | 511630715 |
| UniProt ID | P46977 |
| mRNA ID | NM_001278503 |
| Length | 705 |
| RefSeq Status | VALIDATED |
| Protein sequence is identical to GI:22749415 (mRNA isoform) | |
| dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform b | |
|---|---|
| Refseq ID | NP_001265433 |
| Protein GI | 511630717 |
| UniProt ID | P46977 |
| mRNA ID | NM_001278504 |
| Length | 613 |
| RefSeq Status | VALIDATED |
| MITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSICWAAKCALAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT | |
Gene Information
Entrez Gene ID
Gene Name
STT3A, subunit of the oligosaccharyltransferase complex (catalytic)
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005789 | TAS:Reactome | C | endoplasmic reticulum membrane |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0016020 | IDA:UniProtKB | C | membrane |
| GO:0008250 | ISS:UniProtKB | C | oligosaccharyltransferase complex |
| GO:0004579 | IMP:UniProtKB | F | dolichyl-diphosphooligosaccharide-protein glycotransferase activity |
| GO:0044267 | TAS:Reactome | P | cellular protein metabolic process |
| GO:0043686 | IMP:UniProtKB | P | co-translational protein modification |
| GO:0043687 | TAS:Reactome | P | post-translational protein modification |
| GO:0018279 | IMP:UniProtKB | P | protein N-linked glycosylation via asparagine |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR003674 | Oligosaccharyl transferase, STT3 subunit |
UniProt Annotations
Entry Information
Gene Name
STT3A, subunit of the oligosaccharyltransferase complex (catalytic)
Protein Entry
STT3A_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P46977-1; Sequence=Displayed; Name=2; IsoId=P46977-2; Sequence=VSP_055106; |
| Catalytic Activity | Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine. |
| Disease | Congenital disorder of glycosylation 1W (CDG1W) [MIM |
| Function | Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. |
| Interaction | O43187:IRAK2; NbExp=2; IntAct=EBI-719212, EBI-447733; Q6PDS3:Sarm1 (xeno); NbExp=2; IntAct=EBI-719212, EBI-6117196; Q86WV6:TMEM173; NbExp=2; IntAct=EBI-719212, EBI-2800345; |
| Pathway | Protein modification; protein glycosylation. |
| Similarity | Belongs to the STT3 family. |
| Subcellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Subunit | Component of the oligosaccharyltransferase (OST) complex (By similarity). OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex. {ECO |
| Tissue Specificity | Expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. Expressed in skin fibroblasts (at protein level). |
Identical and Related Proteins
Unique RefSeq proteins for LMP003635 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 22749415 | RefSeq | NP_689926 | 705 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform a |
| 511630717 | RefSeq | NP_001265433 | 613 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform b |
Identical Sequences to LMP003635 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:511630717 | RefSeq | XP_003253400.1 | 613 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform 2 [Nomascus leucogenys] |
| GI:511630717 | RefSeq | XP_003910960.1 | 613 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X2 [Papio anubis] |
| GI:511630717 | RefSeq | XP_004052429.1 | 613 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Gorilla gorilla gorilla] |
| GI:22749415 | RefSeq | XP_008971588.1 | 705 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Pan paniscus] |
| GI:22749415 | RefSeq | XP_008971589.1 | 705 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Pan paniscus] |
| GI:22749415 | RefSeq | XP_008971590.1 | 705 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Pan paniscus] |
| GI:22749415 | RefSeq | XP_009422649.1 | 705 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1 [Pan troglodytes] |
| GI:22749415 | RefSeq | XP_009422650.1 | 705 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1 [Pan troglodytes] |
| GI:22749415 | RefSeq | XP_010351285.1 | 705 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Rhinopithecus roxellana] |
Related Sequences to LMP003635 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:22749415 | GenBank | AAL77539.1 | 705 | transmembrane protein [Homo sapiens] |
| GI:22749415 | GenBank | AAP36824.1 | 706 | Homo sapiens integral membrane protein 1, partial [synthetic construct] |
| GI:22749415 | GenBank | AAX43580.1 | 706 | integral membrane protein 1, partial [synthetic construct] |
| GI:22749415 | GenBank | AAX43581.1 | 706 | integral membrane protein 1, partial [synthetic construct] |
| GI:22749415 | GenBank | ACM84938.1 | 721 | Sequence 10436 from patent US 6812339 |
| GI:511630717 | GenBank | AFH28586.1 | 705 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Macaca mulatta] |
| GI:511630717 | GenBank | AFI35386.1 | 705 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Macaca mulatta] |
| GI:511630717 | GenBank | AFL26126.1 | 705 | Sequence 475 from patent US 8168586 |
| GI:511630717 | GenBank | AGV82153.1 | 705 | Sequence 475 from patent US 8524238 |
| GI:22749415 | RefSeq | XP_002754607.1 | 705 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Callithrix jacchus] |
| GI:511630717 | RefSeq | NP_001244350.1 | 705 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Macaca mulatta] |
| GI:511630717 | RefSeq | XP_005580133.1 | 705 | PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Macaca fascicularis] |