Gene/Proteome Database (LMPD)

LMPD ID
LMP003636
Gene ID
16430
Species
Mus musculus (Mouse)
Gene Name
STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Gene Symbol
Stt3a
Synonyms
AA408947; BB081708; Itm1
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A; B5; STT3-A; integral membrane protein 1; intergral membrane protein 1; oligosaccharyl transferase subunit STT3A
Chromosome
9
Map Location
9 A4|9 20.67 cM
EC Number
2.4.99.18

Proteins

dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
Refseq ID NP_032434
Protein GI 148747128
UniProt ID P46978
mRNA ID NM_008408
Length 705
RefSeq Status PROVISIONAL
MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWGGYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRGLSRT

Gene Information

Entrez Gene ID
16430
Gene Name
STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Gene Symbol
Stt3a
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:MGI C membrane
GO:0008250 ISS:UniProtKB C oligosaccharyltransferase complex
GO:0004579 ISS:UniProtKB F dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0043686 ISS:UniProtKB P co-translational protein modification
GO:0018279 ISS:UniProtKB P protein N-linked glycosylation via asparagine

KEGG Pathway Links

KEGG Pathway ID Description
mmu00510 N-Glycan biosynthesis
ko04141 Protein processing in endoplasmic reticulum
mmu04141 Protein processing in endoplasmic reticulum

Domain Information

InterPro Annotations

Accession Description
IPR003674 Oligosaccharyl transferase, STT3 subunit

UniProt Annotations

Entry Information

Gene Name
STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Protein Entry
STT3A_MOUSE
UniProt ID
P46978
Species
Mouse

Comments

Comment Type Description
Catalytic Activity Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Function Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A (By similarity). {ECO:0000250}.
Pathway Protein modification; protein glycosylation.
Similarity Belongs to the STT3 family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein.
Subunit Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity). {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP003636 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
148747128 RefSeq NP_032434 705 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A

Identical Sequences to LMP003636 proteins

Reference Database Accession Length Protein Name
GI:148747128 DBBJ BAE41290.1 705 unnamed protein product [Mus musculus]
GI:148747128 DBBJ BAE32207.1 705 unnamed protein product [Mus musculus]
GI:148747128 DBBJ BAE31065.1 705 unnamed protein product [Mus musculus]
GI:148747128 GenBank EDL25412.1 705 STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) [Mus musculus]
GI:148747128 GenBank AAI68223.1 705 Stt3a protein [Rattus norvegicus]
GI:148747128 RefSeq NP_001128221.1 705 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Rattus norvegicus]

Related Sequences to LMP003636 proteins

Reference Database Accession Length Protein Name
GI:148747128 DBBJ BAE35910.1 705 unnamed protein product [Mus musculus]
GI:148747128 GenBank EGW01808.1 705 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Cricetulus griseus]
GI:148747128 RefSeq XP_005347095.1 705 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [Microtus ochrogaster]
GI:148747128 RefSeq XP_006242870.1 753 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1 [Rattus norvegicus]
GI:148747128 RefSeq XP_006510101.1 753 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1 [Mus musculus]
GI:148747128 RefSeq XP_007624718.1 705 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X2 [Cricetulus griseus]