Gene/Proteome Database (LMPD)

LMPD ID
LMP004629
Gene ID
18472
Species
Mus musculus (Mouse)
Gene Name
platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Gene Symbol
Pafah1b1
Synonyms
LIS-1; Lis1; MMS10-U; Mdsh; Ms10u; Pafaha
Alternate Names
platelet-activating factor acetylhydrolase IB subunit alpha; LIS-1; PAFAH alpha; PAF-AH alpha; PAF-AH 45 kDa subunit; lissencephaly-1 protein; PAF acetylhydrolase 45 kDa subunit; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1; platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit
Chromosome
11
Map Location
11 B3|11 45.76 cM

Proteins

platelet-activating factor acetylhydrolase IB subunit alpha
Refseq ID NP_038653
Protein GI 7305363
UniProt ID P63005
mRNA ID NM_013625
Length 410
RefSeq Status VALIDATED
MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR

Gene Information

Entrez Gene ID
18472
Gene Name
platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Gene Symbol
Pafah1b1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000235 IEA:Ensembl C astral microtubule
GO:0005938 IEA:Ensembl C cell cortex
GO:0005813 IDA:MGI C centrosome
GO:0005737 IDA:MGI C cytoplasm
GO:0005829 IEA:Ensembl C cytosol
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0030426 IEA:Ensembl C growth cone
GO:0005871 IEA:Ensembl C kinesin complex
GO:0000776 IEA:Ensembl C kinetochore
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0005875 IDA:MGI C microtubule associated complex
GO:0015630 IDA:MGI C microtubule cytoskeleton
GO:0031512 IDA:MGI C motile primary cilium
GO:0043025 IEA:Ensembl C neuronal cell body
GO:0005635 ISS:MGI C nuclear envelope
GO:0048471 IDA:MGI C perinuclear region of cytoplasm
GO:0008017 IDA:MGI F microtubule binding
GO:0051219 IPI:MGI F phosphoprotein binding
GO:0042803 IPI:MGI F protein homodimerization activity
GO:0001675 IMP:MGI P acrosome assembly
GO:0030036 IMP:MGI P actin cytoskeleton organization
GO:0008344 IMP:MGI P adult locomotory behavior
GO:0001667 IMP:MGI P ameboidal cell migration
GO:0048854 IEA:Ensembl P brain morphogenesis
GO:0016477 IMP:MGI P cell migration
GO:0021987 IMP:MGI P cerebral cortex development
GO:0021895 IEA:Ensembl P cerebral cortex neuron differentiation
GO:0021540 IEA:Ensembl P corpus callosum morphogenesis
GO:0051660 IEA:Ensembl P establishment of centrosome localization
GO:0000132 IEA:UniProtKB-HAMAP P establishment of mitotic spindle orientation
GO:0021766 IMP:MGI P hippocampus development
GO:0021819 IGI:MGI P layer formation in cerebral cortex
GO:0007611 IMP:MGI P learning or memory
GO:0016042 IEA:UniProtKB-KW P lipid catabolic process
GO:0000226 IMP:MGI P microtubule cytoskeleton organization
GO:0031023 IEA:Ensembl P microtubule organizing center organization
GO:0007067 IEA:UniProtKB-KW P mitotic nuclear division
GO:0046329 IGI:MGI P negative regulation of JNK cascade
GO:0010977 IEA:Ensembl P negative regulation of neuron projection development
GO:0007405 IMP:MGI P neuroblast proliferation
GO:0050885 IMP:MGI P neuromuscular process controlling balance
GO:0001764 IMP:MGI P neuron migration
GO:0051081 IMP:MGI P nuclear envelope disassembly
GO:0007097 IEA:Ensembl P nuclear migration
GO:0036035 IMP:MGI P osteoclast development
GO:0045773 IEA:Ensembl P positive regulation of axon extension
GO:0001961 IMP:MGI P positive regulation of cytokine-mediated signaling pathway
GO:0045931 IEA:Ensembl P positive regulation of mitotic cell cycle
GO:0009306 IMP:MGI P protein secretion
GO:0032319 IMP:MGI P regulation of Rho GTPase activity
GO:0008090 IDA:MGI P retrograde axon cargo transport
GO:0017145 IEA:Ensembl P stem cell division
GO:0007268 IMP:MGI P synaptic transmission
GO:0019226 IMP:MGI P transmission of nerve impulse
GO:0047496 IGI:MGI P vesicle transport along microtubule

KEGG Pathway Links

KEGG Pathway ID Description
mmu00565 Ether lipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
5893362 Separation of Sister Chromatids

Domain Information

InterPro Annotations

Accession Description
IPR017252 Dynein_regulator_LIS1
IPR020472 G-protein beta WD-40 repeat
IPR006594 LisH dimerisation motif
IPR013720 LisH dimerisation motif, subgroup
IPR019775 WD40 repeat, conserved site
IPR017986 WD40-repeat-containing domain
IPR015943 WD40/YVTN repeat-like-containing domain
IPR001680 WD40_repeat

UniProt Annotations

Entry Information

Gene Name
platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Protein Entry
LIS1_MOUSE
UniProt ID
P63005
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P63005-1, P43035-1; Sequence=Displayed; Name=2; IsoId=P63005-2, P43035-2; Sequence=VSP_006778;
Developmental Stage Embryonic expression begins prior to the blastocyst stage, when maternally expressed protein is depleted. By E10.5, expression is abundant in the developing central and peripheral nervous systems. Major sites of expression include the neuroepithelium of the fore-, mid-, and hindbrain, the spinal cord, the dorsal root and the cranial ganglia. By E13.5, highly expressed in neuroblasts as well as postmitotic neurons of the cortical plate. After completion of neuronal migration expression remains high in the cortex. Also expressed in the testis from P8. {ECO:0000269|PubMed:11001923, ECO:0000269|PubMed:11056530, ECO:0000269|PubMed:11163259, ECO:0000269|PubMed:11231056, ECO:0000269|PubMed:11344260, ECO:0000269|PubMed:12950100, ECO:0000269|PubMed:15147871, ECO:0000269|PubMed:15473966}.
Domain Dimerization mediated by the LisH domain may be required to activate dynein. {ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:15274919, ECO:0000269|PubMed:16258276}.
Function Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein- mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position. {ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:11056530, ECO:0000269|PubMed:11344260, ECO:0000269|PubMed:12796778, ECO:0000269|PubMed:12911752, ECO:0000269|PubMed:14507966, ECO:0000269|PubMed:14578885, ECO:0000269|PubMed:14691133, ECO:0000269|PubMed:15173193, ECO:0000269|PubMed:15473966, ECO:0000269|PubMed:16107726, ECO:0000269|PubMed:16203747, ECO:0000269|PubMed:16369480, ECO:0000269|PubMed:16481446}.
Interaction Q9ERR1:Ndel1; NbExp=4; IntAct=EBI-917499, EBI-646668; Q61205:Pafah1b3; NbExp=2; IntAct=EBI-917499, EBI-1007637;
Similarity Belongs to the WD repeat LIS1/nudF family. {ECO:0000255|HAMAP-Rule:MF_03141}.
Similarity Contains 1 LisH domain. {ECO:0000255|HAMAP- Rule:MF_03141}.
Similarity Contains 7 WD repeats. {ECO:0000255|HAMAP- Rule:MF_03141}.
Subcellular Location Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Nucleus membrane {ECO:0000255|HAMAP-Rule:MF_03141}. Note=May localize to the nuclear membrane (By similarity). Localizes to the plus end of microtubules and to the centrosome. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes. {ECO:0000250}.
Subunit Interacts with DISC1, and this interaction is enhanced by NDEL1 (By similarity). Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Can self-associate. Interacts with DCX, dynein, dynactin, IQGAP1, KATNB1, NDE1, NDEL1, NUDC, and RSN. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling. Interacts with ASUN. {ECO:0000250, ECO:0000269|PubMed:11001923, ECO:0000269|PubMed:11056530, ECO:0000269|PubMed:11163258, ECO:0000269|PubMed:11163259, ECO:0000269|PubMed:11163260, ECO:0000269|PubMed:11231056, ECO:0000269|PubMed:11344260, ECO:0000269|PubMed:11940666, ECO:0000269|PubMed:12885786, ECO:0000269|PubMed:14578885, ECO:0000269|PubMed:15173193, ECO:0000269|PubMed:15473966, ECO:0000269|PubMed:15473967, ECO:0000269|PubMed:15572112, ECO:0000269|PubMed:16203747, ECO:0000269|PubMed:16291865, ECO:0000269|PubMed:16369480, ECO:0000269|PubMed:16481446, ECO:0000269|PubMed:23097494}.
Tissue Specificity Highly expressed in brain, particularly the hippocampus and the olfactory bulb. Also highly expressed in testis, including all seminiferous tubule cell types, all types of spermatogenic and Sertoli cells, and meiotically dividing and elongating spermatids. Expressed at lower levels in heart, kidney, large intestine, liver, lung, ovary, small intestine and spleen. {ECO:0000269|PubMed:11163259, ECO:0000269|PubMed:11163260, ECO:0000269|PubMed:12551946}.

Identical and Related Proteins

Unique RefSeq proteins for LMP004629 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
7305363 RefSeq NP_038653 410 platelet-activating factor acetylhydrolase IB subunit alpha

Identical Sequences to LMP004629 proteins

Reference Database Accession Length Protein Name
GI:7305363 RefSeq XP_008693491.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Ursus maritimus]
GI:7305363 RefSeq XP_008827035.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Nannospalax galili]
GI:7305363 RefSeq XP_008994595.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Callithrix jacchus]
GI:7305363 RefSeq XP_008959733.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Pan paniscus]
GI:7305363 RefSeq XP_009429813.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha isoform X1 [Pan troglodytes]
GI:7305363 RefSeq XP_010341907.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha isoform X1 [Saimiri boliviensis boliviensis]

Related Sequences to LMP004629 proteins

Reference Database Accession Length Protein Name
GI:7305363 GenBank AAG33867.1 410 platelet-activating factor acetylhydrolase Ib-alpha subunit [Sus scrofa]
GI:7305363 RefSeq NP_999415.1 410 platelet-activating factor acetylhydrolase IB subunit alpha [Sus scrofa]
GI:7305363 RefSeq XP_002718919.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Oryctolagus cuniculus]
GI:7305363 RefSeq XP_004475147.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha isoform 1 [Dasypus novemcinctus]
GI:7305363 RefSeq XP_004594029.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Ochotona princeps]
GI:7305363 SwissProt Q9GL51.3 410 RecName: Full=Platelet-activating factor acetylhydrolase IB subunit alpha; AltName: Full=Lissencephaly-1 protein; Short=LIS-1; AltName: Full=PAF acetylhydrolase 45 kDa subunit; Short=PAF-AH 45 kDa subunit; AltName: Full=PAF-AH alpha; Short=PAFAH alpha [Sus scrofa]