Gene/Proteome Database (LMPD)

LMPD ID
LMP004846
Gene ID
68292
Species
Mus musculus (Mouse)
Gene Name
STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Gene Symbol
Stt3b
Synonyms
1300006C19Rik; Simp
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B; STT3-B; B6dom1 antigen; oligosaccharyl transferase subunit STT3B; source of immunodominant MHC-associated peptides
Chromosome
9
Map Location
9 F3|9
EC Number
2.4.99.18

Proteins

dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Refseq ID NP_077184
Protein GI 61651673
UniProt ID Q3TDQ1
mRNA ID NM_024222
Length 823
RefSeq Status PROVISIONAL
MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGTQSASRAAAPKPGPPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNPGNLYDKAGKVRKHVTEQEKPEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRSLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIREGDYFTQQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLGHKPRVTNIVPKQKYLSKKTTKRKRGYVKNKLVFKKGKKTSKKTV

Gene Information

Entrez Gene ID
68292
Gene Name
STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Gene Symbol
Stt3b
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0008250 ISS:UniProtKB C oligosaccharyltransferase complex
GO:0004579 ISS:UniProtKB F dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0030433 ISS:UniProtKB P ER-associated ubiquitin-dependent protein catabolic process
GO:0043686 ISS:UniProtKB P co-translational protein modification
GO:0006516 ISS:UniProtKB P glycoprotein catabolic process
GO:0043687 ISS:UniProtKB P post-translational protein modification
GO:0018279 ISS:UniProtKB P protein N-linked glycosylation via asparagine
GO:0006986 ISS:UniProtKB P response to unfolded protein

KEGG Pathway Links

KEGG Pathway ID Description
mmu00510 N-Glycan biosynthesis
mmu04141 Protein processing in endoplasmic reticulum

Domain Information

InterPro Annotations

Accession Description
IPR003674 Oligosaccharyl transferase, STT3 subunit

UniProt Annotations

Entry Information

Gene Name
STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Protein Entry
STT3B_MOUSE
UniProt ID
Q3TDQ1
Species
Mouse

Comments

Comment Type Description
Catalytic Activity Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Function Catalytic component of oligosaccharyltransferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient cotranslational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation (By similarity). {ECO:0000250}.
Polymorphism In strains 129, C57BL/10, C57BL/6 and LP Glu-776 correlates with a B6dom1-positive phenotype, Asp-776 is found in resistant strains. The B6dom1 minor histocompatibilty antigen (MiHA) is used as a model antigen in studying immunodominance.
Sequence Caution Sequence=AAH13054.2; Type=Erroneous initiation; Evidence={ECO:0000305};
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein.
Subunit Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity). {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP004846 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
61651673 RefSeq NP_077184 823 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

Identical Sequences to LMP004846 proteins

Reference Database Accession Length Protein Name
GI:61651673 DBBJ BAE32968.1 823 unnamed protein product [Mus musculus]
GI:61651673 DBBJ BAE26582.1 823 unnamed protein product [Mus musculus]
GI:61651673 DBBJ BAE31580.1 823 unnamed protein product [Mus musculus]
GI:61651673 GenBank AAH52433.1 823 STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) [Mus musculus]

Related Sequences to LMP004846 proteins

Reference Database Accession Length Protein Name
GI:61651673 DBBJ BAB31390.1 823 unnamed protein product [Mus musculus]
GI:61651673 DBBJ BAE41550.1 823 unnamed protein product [Mus musculus]
GI:61651673 GenBank EDL09283.1 823 STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae), isoform CRA_b [Mus musculus]
GI:61651673 RefSeq XP_005075094.1 824 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [Mesocricetus auratus]
GI:61651673 RefSeq XP_005348110.1 824 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [Microtus ochrogaster]
GI:61651673 SwissProt Q3TDQ1.2 823 RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B; Short=Oligosaccharyl transferase subunit STT3B; Short=STT3-B; AltName: Full=B6dom1 antigen; AltName: Full=Source of immunodominant MHC-associated peptides [Mus musculus]